Hydrogen-Rich Water and Resting Metabolism in Young Adults

NCT07098221 | Not Yet Recruiting DMC

• 1 other identifier: 50-10-18/2024-3
• Study Type: interventional
• Target: 24
• Locations: 0 countries
• Sites: N/A

Brief Summary

The H2REST trial is a randomized, double-blind, placebo-controlled crossover study investigating the acute effects of a single dose of hydrogen-rich water (HRW) on resting metabolism in healthy young adults. Participants complete two testing sessions-receiving either HRW or placebo in a randomized order-followed by standardized metabolic testing. Primary outcomes include resting energy expenditure, respiratory exchange ratio, and substrate utilization measured via indirect calorimetry. The study aims to assess whether acute HRW intake can modulate metabolic rate and fuel preference at rest, and to explore inter-individual variability in response.

Conditions

Healthy Subjects or Volunteers

Keywords

Hydrogen-rich water; Resting metabolism; Energy expenditure; Substrate utilization; Metabolic rate

Outcome Measures

Primary Outcomes (1)

• Resting Energy Expenditure (REE)

  The measurements will be obtained during each study visit. Since this is an interventional study assessing the acute effects of molecular hydrogen, measurements will be collected three times: At baseline, without any intervention (pre-familiarization), After a single dose of molecular hydrogen, and After placebo administration. The period between each intervention will be seven days, serving as a washout period to prevent carry-over effects. All participants will be instructed to maintain their regular diet and to avoid alcohol, caffeine-containing beverages, and strenuous physical activity for at least 24 hours prior to each visit. Measure Type: Continuous (kcal/day) Method: Indirect calorimetry Description: To assess the effect of acute ingestion of hydrogen-rich water versus placebo on resting energy expenditure in healthy young adults.

  Participants will attend three study visits, with each intervention separated by a 7-day washout period to minimize carry-over effects. During each visit, measurements will be conducted approximately 30-60 minutes post-intervention.

Study Arms (2)

ARM1: 250 ml of Hydrogen Rich Water

EXPERIMENTAL

Hydrogen-producing tablets: Tablets designed to dissolve in water and release molecular hydrogen gas, creating hydrogen-rich water that can be consumed to potentially provide antioxidant and anti-inflammatory benefits.

Dietary Supplement: Molecular Hydrogen

ARM 2: 250 ml of Placebo Water (Non-hydrogen-producing tablets)

PLACEBO COMPARATOR

Non-hydrogen-producing tablets, identical in appearance and taste to the active supplement

Dietary Supplement: Placebo Comparator

Interventions

Molecular Hydrogen DIETARY_SUPPLEMENT

Participants in this arm will consume a single 250 mL dose of hydrogen-rich water containing approximately 1.6-2.0 ppm of dissolved molecular hydrogen (H₂), The HRW will be ingested following an overnight fast, and resting metabolism will be measured approximately 30-60 minutes post-consumption using indirect calorimetry.

ARM1: 250 ml of Hydrogen Rich Water

Placebo Comparator DIETARY_SUPPLEMENT

Non-hydrogen-producing tablets, identical in appearance and taste to the active supplement

ARM 2: 250 ml of Placebo Water (Non-hydrogen-producing tablets)

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Aged 18-35 years
• BMI between 18.5 and 25.0 kg/m²
• Apparently healthy, with no history of chronic disease
• Non-smoker, not currently using nicotine-containing products
• Able to provide informed consent
• Willing to refrain from alcohol, caffeine, and strenuous exercise for 24 hours before each study visit
• Able to comply with study procedures, including fasting and attending all study visits

You may not qualify if:

• Diagnosed metabolic, cardiovascular, respiratory, gastrointestinal, or endocrine disorders
• Use of dietary supplements or medications affecting metabolism (within the past 2 weeks)
• Allergy or intolerance to components of the hydrogen or placebo beverages
• Pregnant or lactating
• Shift work or irregular sleep schedule
• Participation in another clinical study within the past 30 days

Sponsors & Collaborators

• University of Novi Sad: lead

MeSH Terms

Interventions

Hydrogen

Intervention Hierarchy (Ancestors)

Elements; Inorganic Chemicals; Gases

Central Study Contacts

Nikola Todorovic, PhD

CONTACT

+381658417576; nikolatodorovic1708@gmail.com

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, INVESTIGATOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Teaching assistant, PhD

Study Record Dates

• First Submitted: July 17, 2025
• First Posted: August 1, 2025
• Study Start: September 1, 2025
• Primary Completion: December 1, 2025
• Study Completion: December 1, 2025
• Last Updated: August 1, 2025

Record last verified: 2025-07

Data Sharing

• IPD Sharing: Will share