The Role of Probiotics and Dietary Interventions in the Treatment of Periodontitis

NCT06944938 | Completed

• 1 other identifier: Dicle University Nutrition
• Study Type: interventional
• Target: 120
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of this study is to evaluate the effects of probiotic supplementation and diet-supported probiotic use on periodontal clinical parameters in individuals with periodontitis. A total of 120 female participants aged between 20-60 years, diagnosed with periodontitis and without systemic diseases, were included in the study. Participants are randomly assigned into three groups: a control group (conventional treatment), a probiotic group (conventional treatment + probiotics), and a diet + probiotic group (conventional treatment + probiotics + personalized diet). Clinical evaluations are performed using measurements of probing depth (PD) and clinical attachment loss (CAL), while dietary intake is assessed using three-day food records. The collected data will be analyzed using SPSS 21.0 software, and a p-value \<0.05 will be considered statistically significant.

Conditions

Periodontal Diseases

Keywords

Periodontitis; probiotics; diet

Outcome Measures

Primary Outcomes (2)

• Change in Probing Depth (PD) from Baseline to 6 Weeks, measured in millimeters (mm)

  Probing depth will be measured at six sites per tooth using a UNC-15 periodontal probe. The mean PD per participant will be calculated, and group-level data will be reported as mean ± standard deviation (SD). PD represents the distance from the gingival margin to the base of the periodontal pocket.

  Baseline and 6 weeks after intervention

• Change in Clinical Attachment Loss (CAL) from Baseline to 6 Weeks, measured in millimeters (mm)

  Clinical attachment loss (CAL) will be assessed at six sites per tooth using a UNC-15 periodontal probe. CAL is defined as the distance from the cemento-enamel junction (CEJ) to the bottom of the pocket. The average CAL per participant will be calculated and presented as mean ± SD at the group level.

  Baseline and 6 weeks after intervention

Secondary Outcomes (2)

• Change in Nutrient Intake (Protein, Fiber, Sugar, and Carbohydrate) from Baseline to 6 Weeks

  Baseline and week 6

• Correlation Between Nutrient Intake and Periodontal Parameters (PD and CAL) at Week 6

  Week 6

Study Arms (3)

control group

EXPERIMENTAL

traditional periodontitis treatment

Procedure: Non-Surgical Periodontal Treatment

Probiotic group

EXPERIMENTAL

traditional periodontitis treatment + probiotic support

Procedure: Non-Surgical Periodontal Treatment; Dietary Supplement: Probiotic Supplementation

Probiotic+diet group

EXPERIMENTAL

traditional periodontitis treatment + probiotic support + diet

Procedure: Non-Surgical Periodontal Treatment; Dietary Supplement: Probiotic Supplementation; Other: Probiotic Supplementation + Personalized Diet

Interventions

Non-Surgical Periodontal Treatment PROCEDURE

Participants in this arm received standard non-surgical periodontal treatment, including scaling and root planing, without any additional supplementation or dietary modification.

Probiotic group; Probiotic+diet group; control group

Probiotic Supplementation DIETARY_SUPPLEMENT

Participants in this arm received standard periodontal treatment along with a daily probiotic supplement (1 capsule/day, containing 10⁹ CFU of Lactobacillus rhamnosus and Bifidobacterium animalis subsp. lactis) for six consecutive weeks.

Probiotic group; Probiotic+diet group

Probiotic Supplementation + Personalized Diet OTHER

Participants in this arm received standard periodontal treatment, the same probiotic protocol as the probiotic group, and a personalized anti-inflammatory diet. The diet was developed by a clinical dietitian and emphasized high-fiber, antioxidant-rich, and prebiotic foods. Consumption of refined carbohydrates, added sugars, and trans fats was restricted.

Probiotic+diet group

Eligibility Criteria

• Age: 20 Years - 60 Years
• Sex: female
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• Female participants aged between 20 and 60 years Clinical diagnosis of periodontitis Presence of at least 20 natural teeth Probing depth between 3 mm and 7 mm (mild to moderate periodontitis) No history of systemic diseases Willingness to participate and provide written informed consent

You may not qualify if:

• Use of antibiotics or probiotics within the past 6 months Current pregnancy or lactation Smoking Presence of systemic conditions such as diabetes or cardiovascular disease Fewer than 20 natural teeth Inability or unwillingness to comply with study procedures

Sponsors & Collaborators

• Dicle University: lead

Study Sites (1)

Private Dental Clinic, Elazığ, Turkey

Elazığ, Diyarbakır, 21280, Turkey (Türkiye)

Location

Related Publications (1)

• Yilmaz FC, Gorgin NC. The role of probiotics and dietary interventions in the treatment of periodontitis: a pilot randomized controlled clinical trial. BMC Oral Health. 2025 Jul 31;25(1):1287. doi: 10.1186/s12903-025-06510-4.

  PMID: 40745652 DERIVED

Related Links

• Related Info

MeSH Terms

Conditions

Periodontal Diseases; Periodontitis

Condition Hierarchy (Ancestors)

Mouth Diseases; Stomatognathic Diseases

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: associate professor

Study Record Dates

• First Submitted: April 10, 2025
• First Posted: April 25, 2025
• Study Start: October 1, 2019
• Primary Completion: October 1, 2020
• Study Completion: October 1, 2021
• Last Updated: April 25, 2025

Record last verified: 2019-10

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)