Inflamation, Gut Leakage, Iron Status After 6000m Ergometer Test in Elite Rowers.

NCT06890741 | Completed

• 1 other identifier: Rowers6000m 2023
• Study Type: observational
• Target: 21
• Locations: 1 country
• Sites: 1

Brief Summary

The influence of a 6000m ergometer test on the markers of gut injury, endotoxemia, iron status, stress and inflammation in elite rowers.

Conditions

Endothenial Dysfunction; Iron Status; Oxidative Stress

Keywords

endotoxin; gut permeability; gastrointestinal function; iron status; oxidative stress

Outcome Measures

Primary Outcomes (11)

• I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury

  I-FABP concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

• LBP (lipopolysaccharide binding protein) to measure endotoxin

  LBP concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

• Claudin 3 to measure tight junction leakage

  claudin 3 concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

• LPS (lipopolysaccharide) to measure endotoxin

  LPS concentration measured in blood \[EU/Ll\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

• Lactic acid to measure fatigue after the race

  Lactate concentration measured in capillary blood from earlobe \[mmol/L\] using a commercially available kit (Diaglobal, Berlin, Germany)

  Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.

• Ferritin to measure iron status

  Ferritin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

• Transferrin to measure iron status

  Transferrin concentration measured in blood \[mg/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

• Hepcidin to measure iron status

  Hepcidin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

• IL-6 (Interleukin-6) to measure inflammation

  IL-6 concentration measured in blood \[ng/l\] using commercially available enzyme-linked immunosorbent assays.

  Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

• HO-1 (Heme Oxygenase 1) to measure oxidative stress

  HO-1 concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

• ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) to measure oxidative stress

  ACSL4 concentration measured in blood \[pg/ml\] using commercially available enzyme-linked immunosorbent assays.

  Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

Secondary Outcomes (11)

• energy

  day before test, in the morning before the test

• protein

  day before test, in the morning before the test

• carbohydrate

  day before test, in the morning before the test

• fat

  day before test, in the morning before the test

• fiber

  day before test, in the morning before the test

Study Arms (1)

elite rowers

Other: exercise test

Interventions

exercise test OTHER

Participant perforemed 6000m ergometer test.

elite rowers

Eligibility Criteria

• Age: 18 Years - 24 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Male rowers age 18 - 24 years.

You may qualify if:

• minimum five years of training,
• five training sessions per week minimum,
• total training time minimum of 240 minutes,
• membership in the Polish Rowing Team,
• finishing the 6000-meter ergometer test.

You may not qualify if:

• antibiotic therapy within the last three months,
• probiotics and prebiotics within the last three months,
• dietary regime,
• gastrointestinal diseases.

Sponsors & Collaborators

• Poznan University of Physical Education: lead

Study Sites (1)

Poznan University of Physical Education

Gorzów Wielkopolski, Lubusz Voivodeship, 66-400, Poland

Location

MeSH Terms

Interventions

Exercise Test

Intervention Hierarchy (Ancestors)

Heart Function Tests; Diagnostic Techniques, Cardiovascular; Diagnostic Techniques and Procedures; Diagnosis; Respiratory Function Tests; Diagnostic Techniques, Respiratory System; Ergometry; Investigative Techniques

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: March 17, 2025
• First Posted: March 24, 2025
• Study Start: November 1, 2023
• Primary Completion: November 10, 2023
• Study Completion: November 10, 2023
• Last Updated: March 27, 2025

Record last verified: 2023-11

Data Sharing

• IPD Sharing: Will not share

Locations

PL (1)