NCT06677749

Brief Summary

The purpose of this study is to evaluate the acute local skeletal muscle inflammatory response to thigh muscle (quadriceps) eccentric muscle actions. Eccentric muscle actions occur when the muscle is lengthening as it contracts. This is a pilot project to assess the degree of inflammatory biomarkers, called myokines, that are released locally in skeletal muscle tissue after an acute bout of resistance-exercise using a resistance-exercise machine called an isokinetic dynamometer.

Trial Health

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Monitor

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
12

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Nov 2024

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

July 5, 2024

Completed
4 months until next milestone

First Posted

Study publicly available on registry

November 7, 2024

Completed
8 days until next milestone

Study Start

First participant enrolled

November 15, 2024

Completed
9 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 31, 2025

Completed
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

November 1, 2025

Completed
Last Updated

November 7, 2024

Status Verified

July 1, 2024

Enrollment Period

9 months

First QC Date

July 5, 2024

Last Update Submit

November 4, 2024

Conditions

Outcome Measures

Primary Outcomes (5)

  • Apelin

    Concentration of apelin in skeletal muscle tissue

    Baseline and 3-hours post-exercise

  • Fibroblast growth factor-21

    Concentration of fibroblast growth factor-21 in skeletal muscle tissue

    Baseline and 3-hours post-exercise

  • Irisin

    Concentration of irisin in skeletal muscle tissue

    Baseline and 3-hours post-exercise

  • Interleukin-6

    Concentration of interleukin-6 in skeletal muscle tissue

    Baseline and 3-hours post-exercise

  • Interleukin-15

    Concentration of interleukin-15 in skeletal muscle tissue

    Baseline and 3-hours post-exercise

Secondary Outcomes (5)

  • Body mass

    Baseline

  • Body height

    Baseline

  • Body mass index

    Baseline

  • Fat mass

    Baseline

  • Fat free mass

    Baseline

Study Arms (1)

Eccentric Exercise

EXPERIMENTAL

300 repetitions of isokinetic dynamometer eccentric knee extensions.

Procedure: Eccentric resistance-exercise

Interventions

Each participant will complete 300 repetitions of eccentric knee extensions using an isokinetic dynamometer after a baseline skeletal muscle biopsy is taken. Three hours following this, a second muscle biopsy will be taken to evaluate the changes in the dependent variables being measured (myokines).

Eccentric Exercise

Eligibility Criteria

Age18 Years - 35 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • years old
  • healthy
  • recreationally active (\>/= 150 minutes of moderate to vigorous physical activity per week for at least the past three months)

You may not qualify if:

  • "yes" answer to the Get Active Questionnaire which screens for health conditions that may affect exercise ability

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Applied Research Centre, Faculty of Kinesiology, University of Manitoba

Winnipeg, Manitoba, R3T2N2, Canada

RECRUITING

Study Officials

  • Stephen M Cornish, PhD

    University of Manitoba

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Stephen M Cornish, PhD

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NA
Masking
NONE
Purpose
BASIC SCIENCE
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

July 5, 2024

First Posted

November 7, 2024

Study Start

November 15, 2024

Primary Completion

July 31, 2025

Study Completion

November 1, 2025

Last Updated

November 7, 2024

Record last verified: 2024-07

Data Sharing

IPD Sharing
Will not share

Locations