Beetroot Juice Effects on Contractile or Neuromuscular Properties in Male Trained Sprinters

NCT06675682 | Completed

• 1 other identifier: UPC_Comillas
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

Beetroot juice is a rich source of nitrate (NO3-), which serves as a precursor to nitric oxide (NO) via the NO3- to nitrite (NO2-) to NO conversion pathway. This pathway is believed to enhance vasodilation and improve neuromuscular function, such as by increasing sarcoplasmic reticulum calcium release and re-uptake, leading to enhanced force production in type II muscle fibers and improved muscle contractile recruitment. Therefore, the aim of this study was to investigate the effects of beetroot juice supplementation on muscle contractile properties, as assessed by tensiomyography, and neuromuscular performance in sprint-trained athletes.

Conditions

Nitrates; Placebo

Keywords

Sport nutrition; Track and field; Tensiomyography; Athletes; Nitrates

Outcome Measures

Primary Outcomes (2)

• Tensiomyography changes (seconds): Delay time (Td) ; Contraction time (Tc) ; Sustain time (Ts) ; Relaxation time (Tr)

  The measurement of the rectus femoris, biceps femoris, gastrocnemius lateralis, and gastrocnemius medialis muscles was conducted using tensiomyography. We analyzed different parameters such as selay time (Td) as a time between the electrical impulse and 10% of the contraction; Contraction time (Tc) as a time between 10% and 90% of the contraction; Sustain time (Ts) as a time between 50% of the contraction and 50% of the relaxation; Relaxation time (Tr) as a time between 90% and 50% of the relaxation.

  1-week

• Changes in 60-m and 100-m sprint test (seconds)

  Time to complete 60-m and 100-m sprint test using photocell timing gates

  1-week

Secondary Outcomes (4)

• Changes in maximal countermovement and squat jump height (cm)

  1-week.

• Changes in nitrites and nitrates levels in saliva (μmol/L)

  1-week

• Changes in side effects questionnarie

  1-week

• Changes in rate of perception effort

  1-week

Study Arms (2)

Beetroot juice supplementation

EXPERIMENTAL

One serving 70 mL of beetroot juice (6.4 mmol of NO3-; Beet-It-Pro Elite Shot, James White Drinks Ltd., Ipswich, UK) 2.5 h before initiating the testing session.

Dietary Supplement: Beetroot Juice (Beet-It Stamina Shot) and Exercise Training

Placebo supplementation

PLACEBO COMPARATOR

One serving 70 mL of beetroot juice placebo (0.04 mmol of NO3-; Beet-It-Pro Elite Shot, James White Drinks Ltd., Ipswich, UK) 2.5 h before initiating the testing session.

Dietary Supplement: Beetroot Juice (Beet-It Stamina Shot) and Exercise Training

Interventions

Beetroot Juice (Beet-It Stamina Shot) and Exercise Training DIETARY_SUPPLEMENT

Player randomization was performed using an online tool (https://www.randomizer.org/). An independent researcher assigned alphanumeric codes to each sequence to ensure blinding of both sprint athletes and researchers during the trials. Each player participated in two identical experimental trials separated by one week to allow physical recovery between testing occasions and washout from the beetroot juice treatment.

Beetroot juice supplementation; Placebo supplementation

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Between 18 to 40 years old.
• With a regular training practise in track and field disciplines \> 5 years.

You may not qualify if:

• Intolerance to beetroot juice or NO3- derivatives
• Presence of any chronic pathology or injury within three months prior to the study-
• Use of medications or supplements (e.g., caffeine) during the study.
• Failure to attend all experimental sessions.
• Failure to adhere to the dietary guidelines established in the study

Sponsors & Collaborators

• Universidad Pontificia Comillas: lead
• Universidad Francisco de Vitoria: collaborator
• Universidad de León: collaborator
• Anglia Ruskin University: collaborator
• Universidad Rey Juan Carlos: collaborator

Study Sites (1)

Universidad Pontificia Comillas

Madrid, Spain

Location

MeSH Terms

Interventions

Exercise

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Alvaro López Samanes, PhD

  Universidad Pontificia Comillas

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: QUADRUPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: TREATMENT
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: August 27, 2024
• First Posted: November 5, 2024
• Study Start: March 17, 2021
• Primary Completion: March 24, 2021
• Study Completion: March 31, 2021
• Last Updated: November 5, 2024

Record last verified: 2024-08

Locations

ES (1)