Resistance Training, Detraining, and Retraining Study 2024 (TraDeRe2024)

NCT06573086 | Recruiting

• 1 other identifier: 1394/13.00.04.00/2023
• Study Type: interventional
• Target: 90
• Locations: 1 country
• Sites: 1

Brief Summary

The goals of our research project are to identify factors explaining inter-individual variation in responses to resistance training (RT) and the baseline determinants underlying an individual's sensitivity to respond to RT. Moreover, investigators aim to assess whether a responsiveness to RT predicts responsiveness to endurance training (ET). Thus, investigators aim to gain a deeper understanding of exercise adaptation processes. The main questions investigators aim to answer are:

• Can the physiological responses of one RT intervention be extrapolated to a subsequent RT intervention?
• If so, what are the mechanisms underlying differing skeletal muscle growth responses in low, and high responders of skeletal muscle hypertrophy?
• If so, do the low responders of skeletal muscle growth respond more favourably when the amount of RT is increased?
• Are the high, moderate, and low responders of RT also the highest, moderate, and lowest responders to ET? To examine these main research questions, high (n=30), low (n=30), and moderate (n=30) responders of skeletal muscle growth in response to RT (intervention I, NCT05874986) are reallocated into a subsequent 12-week RT intervention (intervention II) after a detraining period. A subgroup of these participants (n=10) will engage in a 6-week control period before starting the second RT period. Additionally, after intervention II, participants will participate in an ET intervention, lasting 6 weeks. In this intervention II, reallocated participants will be:
• Resistance training with supervision for 12 weeks
• Consuming deuterium oxide for the assessment of muscle protein synthesis
• Consuming D3-3-methylhistidine for the assessment of acute muscle protein breakdown
• Consuming D3-creatine for the examination of whole-body skeletal muscle mass
• Providing a spot urine sample six (6) times, and urine collection for 24 hours performed twice
• Providing saliva samples (30-32 in total) for the assessment of body water enrichment of deuterium
• Providing a muscle biopsy four or five (4-5) times during the study
• Providing a blood sample fourteen (14) times during the study
• Assessed for body composition and body volume four or five times (4-5) during the study
• Participating in muscle size, maximal dynamic strength and TMS measurements four or five (4-5) times during the study
• Asked to answer questionnaires related to e.g. stress, physical activity, sleep, perceived exertion, and diet
• Participating in recovery measurements before and after the second-to-last and the last RT bout, and once in the days between these RT bouts, consisting of six (6) body volume measurements and six (6) maximal voluntary isometric contraction (kg) tests using horizontal leg press for the assessment of neuromuscular recovery
• Participating in an acute resistance exercise (RE) after the 12-week RT intervention. Furthermore, in the ET intervention, participants will be:
• Participating in a familiarization session and resting electrocardiograph measurements before the intervention
• Participating in endurance testing consisting of body composition, movement economy, and incremental RAMP testing before and after ET intervention
• Endurance training with supervision for 6 weeks, three times a week.

Conditions

Hypertrophy; Exercise Training; Skeletal Muscle Atrophy; Healthy

Outcome Measures

Primary Outcomes (1)

• Change in m. vastus lateralis (VL) cross-sectional area (CSA) with ultrasound (US)

  VL CSA (cm\^2) is determined at mid-thigh using a B-mode axial plane US (Venue Fit R4, GE Medical Systems, USA) with a L4-20t-RS linear-array probe (48,43 mm width) in extended-field-of-view mode (6/12 MHz, 39 frames per second). Additionally, VL CSA (cm\^2) will also be determined at baseline with different US, at mid-thigh using a B-mode axial plane US (model SSD-α10, Aloka, Tokyo, Japan) with a 13 MHz linear-array probe (60 mm width) in extended-field-of-view mode (23 Hz sampling frequency). Aloka-US was used in the intervention I, but GE-US in intervention II due to the progressive malfunctioning of Aloka. Therefore, both machines are used in baseline measurements to calculate the plausible inter-US variability. The CSA will be measured using the polygon function in ImageJ software. Per each measurement, three images will be analysed from which the average from the two closest ones will be calculated.

  Baseline (test and retest), week 7, week 13

Secondary Outcomes (47)

• Muscle protein synthesis (MPS) with the Combined Oral Stable Isotope Assessment of Muscle (COSIAM)

  From baseline to week 7 (6 weeks of RT in duration)

• Muscle protein breakdown (MPB) with the Combined Oral Stable Isotope Assessment of Muscle (COSIAM)

  Baseline, Week 7

• Change in whole-body skeletal muscle mass (SMM) with the Combined Oral Stable Isotope Assessment of Muscle (COSIAM)

  Baseline, Week 7

• Change in muscle thickness (MT) of the elbow flexors after 6 and 12-week resistance training

  Baseline (test and retest), week 7, week 13

• Change in lower and upper limb maximal strength after 6 and 12-week resistance training

  Baseline (test and retest), week 7, week 13,

Study Arms (3)

High responders (n=30)

EXPERIMENTAL

This group consists of participants with the highest increase in skeletal muscle hypertrophy of m. vastus lateralis in response to the initial 12 weeks of RT (Intervention I).

Other: Resistance Training

Moderate responders (n=30)

EXPERIMENTAL

This group consists of participants with the moderate increase in skeletal muscle hypertrophy of m. vastus lateralis in response to the initial 12 weeks of RT (Intervention I).

Other: Resistance Training

Low Responders (n=30)

EXPERIMENTAL

This group consists of participants with the lowest increase in skeletal muscle hypertrophy of m. vastus lateralis in response to the initial 12 weeks of RT (Intervention I).

Other: Resistance Training

Interventions

Resistance Training OTHER

12-week resistance training

Also known as: Exercise Training

High responders (n=30); Low Responders (n=30); Moderate responders (n=30)

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• age 18-50
• healthy (e.g., no diagnosed type 2 diabetes, cardiovascular disease, musculoskeletal disorders, etc.)
• prior participation in intervention I (NCT05874986)

You may not qualify if:

• medication affecting the cardiovascular system or metabolism
• metabolic, musculoskeletal, cardiovascular, or other diseases or disorders which may preclude the ability to perform exercise training and testing

Sponsors & Collaborators

• University of Jyvaskyla: lead
• Academy of Finland: collaborator

Study Sites (1)

University of Jyväskylä

Jyväskylä, Central Finland, 40014, Finland

RECRUITING

MeSH Terms

Conditions

Hypertrophy; Muscular Atrophy

Interventions

Resistance Training; Exercise

Condition Hierarchy (Ancestors)

Pathological Conditions, Anatomical; Pathological Conditions, Signs and Symptoms; Neuromuscular Manifestations; Neurologic Manifestations; Nervous System Diseases; Atrophy; Signs and Symptoms

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Juha P Ahtiainen, Assoc.Prof.

  University of Jyväskylä, Faculty of Sport and Health Sciences

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Juha P Ahtiainen, Assoc.Prof.

CONTACT

+358408053740; juha.ahtiainen@jyu.fi

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: July 29, 2024
• First Posted: August 27, 2024
• Study Start: August 19, 2024
• Primary Completion: June 15, 2025
• Study Completion: June 15, 2025
• Last Updated: April 6, 2025

Record last verified: 2025-04

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, SAP, ICF, CSR, ANALYTIC CODE
• Time Frame: Immediately following publication. No end date.
• Access Criteria: Researchers who provide a methodologically sound proposal. Proposals should be directed to juha.ahtiainen@jyu.fi

Locations

FI (1)