Osteopontin Gene Polymorphism in Stroke Patients in Egypt

NCT06462599 | Not Yet Recruiting

• 1 other identifier: Gene polymorphisms in stroke
• Study Type: observational
• Target: 100
• Locations: 0 countries
• Sites: N/A

Brief Summary

This study aims to investigate the correlation of serum osteopontin level as a predictior and a prognostic factor in upper egyptian patients and correlation between Osteopontin Gene Polymorphisms and serum level of osteopontin in ischaemic stroke patients

Conditions

Ischemic Stroke

Outcome Measures

Primary Outcomes (1)

• Measure osteopontin level in ischaemic stroke patients and different gene polymorphisms related to the disease

  investigate the correlation of serum osteopontin level as a predictior and a prognostic factor in upper egyptian patients. Correlation between Osteopontin Gene Polymorphisms and serum level of osteopontin in ischaemic stroke patients

  Baseline

Study Arms (2)

Cases

Patients with ischaemic stroke

Genetic: Gene polymorphism from blood samples

Control

Healthy individuals

Genetic: Gene polymorphism from blood samples

Interventions

Gene polymorphism from blood samples GENETIC

Blood samples will be collected from stroke patients within 24 hrs of stroke and normal volunteers. Two samples will be collected serum and plasma; five ml whole blood from patients and normal volunteers and centrifuge serum samples at 1500 rpm 10 min then will be stored at - 80 °C until the day of the analysis. Plasma samples will be stored at - 80 °C until the day of the analysis without centrifugation

Cases; Control

Eligibility Criteria

• Age: 18 Years - 70 Years
• Sex: all
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

we will enrol 72 participants; 36 stroke patients and 36 control cases (age matched) recruited among the attendants of Neurology department, Assiut University Hospital, adult age.

You may qualify if:

• For acute ischemic stroke cases:
• ( Acute ischemic stroke will be defined as an episode of focal neurological deficits lasting for more than 24 hour with relevant lesion in brain computerized tomography (CT) or magnetic resonance (MR) image\>)
• both sex 2- Age between 18 to 70 years old. 3-symptoms suggestive of acute ischemic stroke: presenting within 24 hours of onset of these symptoms
• For old ischemic stroke:
• both sex
• Age between 18 to 70 years old.
• Duration of3to 6 month of development of ischemic symptoms
• For control cases:
• both sex
• Healthy people
• Age: above 18 years old

You may not qualify if:

• Patients with a previous history of stroke; Patients with hemorrhagic stroke. Patients with coronary heart disease, heart failure, chronic inflammation, intracranial infection/brain tumor and malignant tumor.
• Patients with liver, kidney and other important organ dysfunction. Patients with severe abnormal coagulation function

Sponsors & Collaborators

• Assiut University: lead

Related Publications (3)

• Jing M, Li B, Hou X, Shoba J, Li C, Liang H, Zhang X, Liu E, Yang B, Meng X. OPN gene polymorphism and the serum OPN levels confer the susceptibility and prognosis of ischemic stroke in Chinese patients. Cell Physiol Biochem. 2013;32(6):1798-807. doi: 10.1159/000356613. Epub 2013 Dec 13.

  PMID: 24355932 BACKGROUND

• Meseguer E, Diallo D, Labreuche J, Charles H, Delbosc S, Mangin G, Monteiro Tavares L, Caligiuri G, Nicoletti A, Amarenco P. Osteopontin Predicts Three-Month Outcome in Stroke Patients Treated by Reperfusion Therapies. J Clin Med. 2020 Dec 13;9(12):4028. doi: 10.3390/jcm9124028.

  PMID: 33322093 BACKGROUND

• Zhang Y, Wang JR, Zhang EN, Zhao ZJ. Analysis of the effect of changes in serum osteopontin levels on patients with acute cerebral infarction. Pak J Med Sci. 2024 Mar-Apr;40(4):718-722. doi: 10.12669/pjms.40.4.7045.

  PMID: 38544995 BACKGROUND

Biospecimen

Retention: SAMPLES WITH DNA

Blood samples will be collected from stroke patients within 24 hrs of stroke and normal volunteers. Two samples will be collected serum and plasma; five ml whole blood from patients and normal volunteers and centrifuge serum samples at 1500 rpm 10 min then will be stored at - 80 °C until the day of the analysis. Plasma samples will be stored at - 80 °C until the day of the analysis without centrifugation

MeSH Terms

Conditions

Ischemic Stroke

Condition Hierarchy (Ancestors)

Stroke; Cerebrovascular Disorders; Brain Diseases; Central Nervous System Diseases; Nervous System Diseases; Vascular Diseases; Cardiovascular Diseases

Study Officials

• Michel Effat, Lecturer

  Researcher

  STUDY DIRECTOR

Central Study Contacts

Madonna Nabil, Demonstrator

CONTACT

01285958827; nabilmagy69@gmail.com

Thoraya Eldeeb, Professor

CONTACT

01060566244; thyriaeldeeb49@gmail.com

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant lecturer

Study Record Dates

• First Submitted: June 12, 2024
• First Posted: June 17, 2024
• Study Start: September 1, 2024
• Primary Completion: September 1, 2024
• Study Completion: October 1, 2025
• Last Updated: June 17, 2024

Record last verified: 2024-06