Phenotypic and Genotypic Characteristics of Pseudomonas Aeruginosa Isolates in Sohag University Hospitals

NCT06417593 | Recruiting

• 1 other identifier: Soh-Med-24-04-016MS
• Study Type: observational
• Target: 100
• Locations: 1 country
• Sites: 1

Brief Summary

Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions from Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections that will be obtained under complete aseptic precautions.

Conditions

Pseudomonas Aeruginosa Infection

Outcome Measures

Primary Outcomes (4)

• Isolation and identification of pseudomonas aeruginosa causing different nosocomial infections.

  Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions

  6 months

• Identification of antibiotic susceptibility pattern of isolated strains of pseudomonas

  Antibiotic susceptibility testing of the isolates will be performed using disc diffusion method according to clinical laboratory standards institute (CLSI) guidelines

  6 months

• Detection of biofilm forming isolates

  Detection of biofilm forming isolates by tissue culture plate method.

  6 months

• Detection of virulence and antibiotic resistance genes of pseudomonas aeruginosa.

  Detection of virulence and antibiotic resistance genes by conventional Polymerase Chain Reaction

  6 months

Study Arms (1)

group1

Pseudomonas Aeruginosa Isolates

Eligibility Criteria

• Age: 1 Year - 80 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections will be obtained under complete aseptic precautions.

You may qualify if:

• Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections will be obtained under complete aseptic precautions.

You may not qualify if:

• Any infection before 48 hours of patient admission at Hospital

Sponsors & Collaborators

• Sohag University: lead

Study Sites (1)

Faculty of medicine Sohag university hospital

Sohag, Egypt

RECRUITING

MeSH Terms

Conditions

Pseudomonas Infections

Condition Hierarchy (Ancestors)

Gram-Negative Bacterial Infections; Bacterial Infections; Bacterial Infections and Mycoses; Infections

Central Study Contacts

Martina Gamil Fayez, Demonstrator at Microbiology

CONTACT

01272602453; martina.gameel@med.sohag.edu.eg

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: principle investigator

Study Record Dates

• First Submitted: May 11, 2024
• First Posted: May 16, 2024
• Study Start: April 1, 2024
• Primary Completion: November 1, 2024
• Study Completion: March 1, 2025
• Last Updated: May 20, 2024

Record last verified: 2024-05

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL

Locations

EG (1)