Rapid Detection of Pseudomonas Aeruginosa in Bronchoalveolar Lavage Fluid Using Label-free Single-particle Imaging Technology and Assessment of Post-treatment Efficacy in Patients With Pseudomonas Aeruginosa Infection

NCT06986512 | Not Yet Recruiting

• 1 other identifier: 2024-SR-806
• Study Type: observational
• Target: 100
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of this study is to observe specimens of bronchoalveolar lavage fluid from patients using a reflection enhanced dark-field scattering microscopy. By observing parameters such as the size, morphology, scattering intensity, and movement speed of pathogens, combined with the clinical characteristics of patients, a rapid diagnosis of Pseudomonas aeruginosa infection can be made. At the same time, the bronchoalveolar lavage fluid samples of patients infected with Pseudomonas aeruginosa before and after treatment were compared, and the quantity and vitality of Pseudomonas aeruginosa were observed to judge the efficacy of antibiotics.

Conditions

Rapid Detection; Pseudomonas Aeruginosa Infection

Outcome Measures

Primary Outcomes (3)

• The size of pathogens

  Measure the size of pathogens

  From enrollment to the end of treatment at 2 weeks

• scattering intensity of pathogen

  Determine the shape of pathogens through scattering intensity

  From enrollment to the end of treatment at 2 weeks

• movement speed of pathogens

  Measure the movement speed of pathogens, such as flagellates like Pseudomonas aeruginosa, which have a fast movement speed

  From enrollment to the end of treatment at 2 weeks

Interventions

bronchoscopy PROCEDURE

We will perform bronchoscopy and collect bronchoalveolar lavage fluid (BALF)

Eligibility Criteria

• Age: 18 Years - 90 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Suspected or diagnosed patients with pulmonary infection caused by Pseudomonas aeruginosa

You may qualify if:

• Aged 18 years and above
• After admission, Pseudomonas aeruginosa was cultured in bronchoalveolar lavage fluid or lower respiratory tract aspirates

You may not qualify if:

• Age under 18 years old or over 90 years old
• Pregnancy, lactation period -

Sponsors & Collaborators

• The First Affiliated Hospital with Nanjing Medical University: lead

Study Sites (1)

The First Affiliated Hospital of Nanjing Medical University

Nanjing, Jiangsu, 21000, China

Location

Biospecimen

Retention: SAMPLES WITH DNA

The investigators will dynamically and continuously collect bronchoalveolar lavage fluid samples from participants and diagnosed with Pseudomonas aeruginosa infection.

MeSH Terms

Conditions

Pseudomonas Infections

Interventions

Bronchoscopy

Condition Hierarchy (Ancestors)

Gram-Negative Bacterial Infections; Bacterial Infections; Bacterial Infections and Mycoses; Infections

Intervention Hierarchy (Ancestors)

Diagnostic Techniques, Respiratory System; Diagnostic Techniques and Procedures; Diagnosis; Endoscopy; Diagnostic Techniques, Surgical; Minimally Invasive Surgical Procedures; Surgical Procedures, Operative; Pulmonary Surgical Procedures; Thoracic Surgical Procedures

Central Study Contacts

Wenkui Sun, Dr

CONTACT

+86 13914742215; sunwenkui@njmu.edu.cn

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Target Duration: 28 Days
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: March 11, 2025
• First Posted: May 23, 2025
• Study Start: September 1, 2025
• Primary Completion: November 30, 2025
• Study Completion: November 30, 2025
• Last Updated: August 14, 2025

Record last verified: 2025-05

Locations

CN (1)