The Associations Between Gut Length, Gut Microbiota and Food Assimilation

NCT06377956 | Completed

• 1 other identifier: SIAT-IRB-240415-H0717
• Study Type: observational
• Target: 42
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this observational study is to explore the relationships between gut length, the microbiota and food energy assimilation rates in humans.

Conditions

Healthy Nutrition; Food Preferences; Metabolic Syndrome, Protection Against

Keywords

Metabolism; Microbiome/microbiota; Body composition; Food intake; Food preference

Outcome Measures

Primary Outcomes (5)

• Gut length

  Volunteers will be scanned Magnetic Resonance Imaging(Shanghai united imaging, uMR 790).

  Through study completion for one year, will be measured on arrival.

• Energy intake

  Response on standard feeding table, the food consumption will be recorded continuously by balances underneath each food dish. The total energy intake will be calculated in KJ (kilojoule).

  Through study completion for one year, will be measured on arrival.

• Fecal energy

  Measuring the heat produced by burning dried feces using an oxygen bomb calorimeter (Parr6400). The total energy per gram tissue will be calculated in kJ (kilojoule)/g.

  Through study completion for one year, will be measured on arrival

• Microbiome/Microbiota

  Abundance of gut microbiome will be from Metagenomic profiling of feces by Illumina.

  Through study completion for one year, will be measured on arrival.

• Metabolites

  Abundance of metabolites will be from metabolomic profiling of feces by LC-MS (liquid chromatography mass spectrometry).

  Through study completion for one year, will be measured on arrival.

Secondary Outcomes (20)

• Urine energy content

  Through study completion for one year, will be measured on arrival.

• Body weight

  Through study completion for one year, will be measured on arrival.

• Waist circumferences

  Through study completion for one year, will be measured on arrival.

• Height

  Through study completion for one year, will be measured on arrival.

• Bone mass

  Through study completion for one year, will be measured on arrival.

Study Arms (1)

Age between 18-40 years old, 100subjects（50 female and 50 man）

He Shuang(Polyethylene Glycol Electrolytes Powder):1/1.5 sachets of PEG(68.56-102.84g dissolved in water with1/1.5L ) were provided to volunteers based on body weight(BW): BW\<60 kg, 1 sachet , BW≥60 kg, 1.5 sachets, drinking an average of 200/250 mL every 15 minutes. It will cause frequent bowel motions or diarrhoea for a couple of hours afterwards. Raceanisodamine Hydrochloride Injection: intramuscular injection of Raceanisodamine Hydrochloride Injection with 10mg. It may cause dry mouth, red face and blurred vision. The half-life of the medicine is 40 minutes and most of the above symptoms disappear within 1 to 3 hours.

Other: Observation without intervention

Interventions

Observation without intervention OTHER

Observation of gut length, food intake, fecal and urine calories, body composition, microbiota, metabolic rate, metabolomics.

Age between 18-40 years old, 100subjects（50 female and 50 man）

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

100 individuals, 50 female and 50 male

You may qualify if:

• Age between 18-40 years old, healthy and disease free.
• Regular bowel movements.

You may not qualify if:

• Individuals who are constipated or suffer from intestinal disorders;
• Individuals with mental illnesses such as depression and claustrophobia;
• Individuals with glaucoma and prostate hypertrophy;
• Metal implants in the body;
• Pregnant and lactating women;
• Individuals with blood phobia, pathologic hypotension or hypertension;
• Use of antibiotics, probiotics, oral laxatives within 8 weeks;
• Individuals with metabolic diseases (e.g., chronic diseases such as diabetes mellitus); recent weight loss due to various medical reasons;
• Individuals with infectious diseases (e.g. HIV);
• Irregular diet, lifestyle, addiction to tobacco, alcohol or drugs.

Sponsors & Collaborators

• Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences: lead

Study Sites (1)

Shenzhen Institute of Advanced Technology

Shenzhen, Guangdong, 518055, China

Location

MeSH Terms

Conditions

Food Preferences; Metabolic Syndrome

Interventions

Observation; Methods

Condition Hierarchy (Ancestors)

Feeding Behavior; Behavior; Insulin Resistance; Hyperinsulinism; Glucose Metabolism Disorders; Metabolic Diseases; Nutritional and Metabolic Diseases

Intervention Hierarchy (Ancestors)

Investigative Techniques

Study Officials

• John R Speakman, PhD

  Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences

  STUDY CHAIR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor, chief researcher

Study Record Dates

• First Submitted: April 17, 2024
• First Posted: April 22, 2024
• Study Start: May 15, 2024
• Primary Completion: June 1, 2025
• Study Completion: June 30, 2025
• Last Updated: January 22, 2026

Record last verified: 2026-01

Data Sharing

• IPD Sharing: Will not share

Locations

CN (1)