Acute Resistance Exercise and Hydrolyzed Collagen Supplementation

NCT06236659 | Completed

• 1 other identifier: 19/SPS/049
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in middle-aged males and females.

Conditions

Diet; Food; Nutrition

Keywords

Hydrolyzed collagen; Resistance Exercise; Collagen Synthesis; Proline; Glycine

Outcome Measures

Primary Outcomes (2)

• Change in a marker of collagen synthesis

  Serum procollagen type Ⅰ N-terminal propeptide (PⅠNP) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistance exercise (RE) during all interventions.

  At rest immediately prior to HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE

• Change in a marker of collagen breakdown

  Plasma β-isomerized C-terminal telopeptide of type I collagen (β-CTX) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistance exercise (RE) during all interventions.

  At rest, immediately prior to HC ingestion, 0.5-hour post RE, 2-hour post RE and 6-hour post RE

Secondary Outcomes (2)

• Estrogen concentrations in women in each trial

  At rest immediately prior to HC ingestion

• Changes in amino acids concentrations in blood

  At rest immediately prior to HC ingestion, 0.5-hour post HC ingestion, 1-hour post HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE

Study Arms (2)

Intervention for middle-aged men: Consumption of hydrolyzed collagen (HC) with resistance exercise

EXPERIMENTAL

Middle-aged male participants consumed one of three different HC doses (0 grams, 15 grams, or 30 grams) with 4 sets of 10 repetitions of leg press exercise at 10-repetition maximum load in a random order and a seven-day wash-out period interspersed between each trial.

Dietary Supplement: Consumption of three different doses of hydrolyzed collagen (HC) with resistance exercise

Intervention for middle-aged women: Consumption of hydrolyzed collagen (HC) with resistance exercise

EXPERIMENTAL

The intervention procedure is exactly same as Arm 1 except for the number of visits and doses of HC. Middle-aged female participants were asked to visit the laboratory on the day of highest oestrogen (i.e. ovulation) and provided with 0 g or 30 g HC. Dates for the trials were determined based on self-report of onset of menses and previous menstrual cycle length.

Dietary Supplement: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise

Interventions

Consumption of three different doses of hydrolyzed collagen (HC) with resistance exercise DIETARY_SUPPLEMENT

Each intervention lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula. All interventions were performed at the same time of day (08:00 - 15:00). Different doses of HC (0 grams, 15 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams and 15.4 grams maltodextrin was used in 0 grams HC and 15 grams HC respectively. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses. The details of dietary supplements used are as follows: Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)

Also known as: Resistance exercise and collagen dose-response for middle-aged male participants

Intervention for middle-aged men: Consumption of hydrolyzed collagen (HC) with resistance exercise

Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise DIETARY_SUPPLEMENT

Each intervention lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula. All interventions were performed at the same time of day (08:00 - 15:00). Different doses of HC (0 grams, and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams and 15.4 grams maltodextrin was used in 0 grams HC and 15 grams HC respectively. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses. The details of dietary supplements used are as follows: Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)

Also known as: Resistance exercise and collagen dose-response for middle-aged female participants

Intervention for middle-aged women: Consumption of hydrolyzed collagen (HC) with resistance exercise

Eligibility Criteria

• Age: 40 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• At least 12 months experienced in resistance exercise (including weekly lower body exercise)
• Free from musculoskeletal injury

You may not qualify if:

• Having a history of patellar tendon pathology
• Vegan
• Consumed nutritional supplements or medication purported to have beneficial effects on muscle-tendon properties (e.g. antioxidants, protein, etc.)
• Had a sustained a lower limb injury in the previous six months
• Smoker/vaper
• Having reached menopause (only for middle-aged female participants)

Sponsors & Collaborators

• Rob Erskine: lead
• University of East Anglia: collaborator

Study Sites (1)

Dr Rob Erskine

Liverpool, L3 3AF, United Kingdom

Location

Study Officials

• Robert M Erskine

  Liverpool John Moores University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Masking Details: Before commencing each experimental intervention, a laboratory technician (independent to the study) made up the three doses of hydrolyzed collagen (HC) and randomly assigned the order of HC dose (Excel 2016, Microsoft, Washington, USA) for each participant. Also, for each intervention, the technician recorded the date, randomly allocated trial number (1, 2 or 3) and corresponding HC dose to blind the investigator.
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Principal Investigator

Model Details: Double-blind, randomized cross-over design

Study Record Dates

• First Submitted: January 24, 2024
• First Posted: February 1, 2024
• Study Start: December 1, 2019
• Primary Completion: September 30, 2021
• Study Completion: September 30, 2023
• Last Updated: February 2, 2024

Record last verified: 2024-01

Data Sharing

• IPD Sharing: Will not share

Locations

GB (1)