Erythrocyte Transport of Lactate During Exercise (TELE Project)

NCT06104150 | Recruiting

• 1 other identifier: PINV-18-ENW9ML-92-B2287R
• Study Type: interventional
• Target: 84
• Locations: 1 country
• Sites: 2

Brief Summary

The goals of this clinical trial are:

• In phase I, trained participants will perform one maximal incremental test and one intervallic submaximal test with a final active recovery.
• Phase II, levels of the MCT1 protein in the erythrocyte membrane will be quantified from trained and sedentary participants.

Conditions

Energy Metabolism

Keywords

lactate kinetics; MCT1; erythrocyte lactate; T1470A polymorphism; exercise

Outcome Measures

Primary Outcomes (2)

• Plasma-Erythrocyte lactate difference

  Difference between lactate concentration inside the erythrocytes and lactate concentration in plasma, expressed in mMol/L (Millimoles Per Liter), from venous blood extracted during the intervallic test from the antecubital vein.

  Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years

• Level of Monocarboxylate Transporter 1 in erythrocytes membrane

  Protein abundance of the monocarboxylate transporter 1 in erythrocyte membranes. It is measured using flow cytometry, from venous blood extracted from the forearm in rest state.

  Through study completion, average of 5 years, an average of 2 months per participant

Secondary Outcomes (4)

• Erythrocyte lactate concentration

  Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years

• Plasma lactate concentration

  Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years

• Blood lactate concentration

  Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years

• Blood pH

  Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years

Other Outcomes (2)

• Genotype for the rs1049434 polymorphism of the SLC16A1 (MCT1) gene

  Through sample recruitment, average of 5 years, an average of 1 month per participant

• Physical Activity level

  Through sample recruitment, average of 5 years, an average of 1 month per participant

Study Arms (1)

Intervallic submaximal test

EXPERIMENTAL

Intervallic exercise test

Other: Intervallic submaximal exercise test

Interventions

Intervallic submaximal exercise test OTHER

Intervallic submáximal exercise test on a cycle ergometer: 5 min warm-up at 80% of First Lactate Threshold (LT1), and subsequent sets of 1.5 min at 20% above the Second Lactate Threshold (LT2) alternated with recoveries of 5 min at 80% of LT1, ending with a 20 min recovery at 80% of LT1.

Also known as: Interval exercise training

Intervallic submaximal test

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• BMI between 18 and 25 kg/m2
• Not smoker
• In the case of trained participants: training at least 5 hours/week, of which at least 3 must be aerobic/anaerobic endurance training. Presentation of a medical certificate attesting to their medical fitness (capability) to perform intense physical exercise.
• In the case of sedentary participants, less than 1 day of training per week or less than 2 hours of structured exercise per week.

You may not qualify if:

• Presenting any pathology that could impede the correct performance of the tests, or could influence the main study variables.
• In the case of the female sample, having irregular menstrual cycles, oligomenorrhea or amenorrhea.

Sponsors & Collaborators

• Technical University of Madrid: lead

Study Sites (2)

Laboratorio de Pediatría. School of Medicine. Univesidad de Cantabria

Santander, Cantabria, 39011, Spain

RECRUITING

Laboratorio de Fisiología del Esfuerzo. Facultad de Ciencias de la Actividad Física y del Deporte (INEF). Universidad Politécnica de Madrid

Madrid, Madrid, 28040, Spain

RECRUITING

Related Publications (1)

• Benitez-Munoz JA, Rojo-Tirado MA, Benito Peinado PJ, Murias JM, Gonzalez-Lamuno D, Cupeiro R. Greater Relative First and Second Lactate Thresholds in Females Compared With Males: Consideration for Exercise Prescription. Int J Sports Physiol Perform. 2024 Oct 28;20(1):30-36. doi: 10.1123/ijspp.2024-0079. Print 2025 Jan 1.

  PMID: 39467538 DERIVED

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Rocío Cupeiro, PhD

  Universidad Politecnica de Madrid

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Rocío Cupeiro, PhD

CONTACT

+34910677990; rocio.cupeiro@upm.es

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Physical Activity and Sports PhD (Associate Professor)

Study Record Dates

• First Submitted: October 19, 2023
• First Posted: October 27, 2023
• Study Start: February 3, 2020
• Primary Completion (Estimated): June 30, 2027
• Study Completion (Estimated): July 31, 2027
• Last Updated: September 12, 2025

Record last verified: 2025-09

Locations

ES (2)