Lactate Profile and Fat Oxidation During Exercise
LacFat
Relationship Between Lactate Profile and Fat Oxidation During Exercise
1 other identifier
interventional
19
1 country
1
Brief Summary
The goal of this clinical trial is to test whether changes in lactate kinetics during exercise (due to glycogen depletion or hyperthermia) alter the pattern of fat oxidation during a maximal incremental cycle ergometer test in healthy young active people. The main questions it aims to answer are:
- Will a rightward shift in lactate kinetics, induced by a previous glycogen depletion, produce a rightward shift in fat oxidation?
- Will a leftward shift in lactate kinetics due to an increase in ambient temperature produce a leftward shift in fat oxidation? Participants will perform three maximal incremental tests in three different conditions:
- one in the control condition;
- one with glycogen depletion;
- and one with ambient heat (the latter two in randomized, counterbalanced order).
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at below P25 for not_applicable
Started Jan 2023
Shorter than P25 for not_applicable
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
First Submitted
Initial submission to the registry
January 11, 2023
CompletedFirst Posted
Study publicly available on registry
January 27, 2023
CompletedStudy Start
First participant enrolled
January 28, 2023
CompletedPrimary Completion
Last participant's last visit for primary outcome
May 11, 2023
CompletedStudy Completion
Last participant's last visit for all outcomes
May 11, 2023
CompletedSeptember 13, 2023
September 1, 2023
3 months
January 11, 2023
September 12, 2023
Conditions
Keywords
Outcome Measures
Primary Outcomes (2)
Blood lactate concentration
Lactate concentration, expressed in mMol/L (Millimoles Per Liter), measured in capillary blood using a lactate analyser. Lactate concentration is measured in the capillary blood extracted from the fingertip, during the tests performed in the different situations (control, glycogen depletion and hyperthermia)
Through study completion, an average of 5 months
Fat oxidation
Fat oxidation, expressed in g/min, is indirectly measured through the analysis of the gas composition of breathing (O2 and CO2), using a gas analyser. Fat oxidation is measured during the tests performed in the different situations (control, glycogen depletion and hyperthermia)
Through study completion, an average of 5 months
Secondary Outcomes (2)
Heart Rate
Through study completion, an average of 5 months
Power output
Through study completion, an average of 5 months
Study Arms (3)
Control
ACTIVE COMPARATORMaximal test on a cycle ergometer
Hyperthermia
EXPERIMENTALMaximal test on a cycle ergometer at 35º-38º C room temperature
Glycogen depletion
EXPERIMENTALMaximal test on a cycle ergometer after a glycogen depletion protocol
Interventions
Maximal incremental exercise test on a cycle ergometer (5 min warm-up at 30W, and subsequent steps of 3 min with power increases of 30W)
Maximal incremental exercise test on a cycle ergometer (5 min warm-up at 30W, and subsequent steps of 3 min with power increases of 30W) performed at a room temperature of 35-38ºC (degrees Celsius).
Maximal incremental exercise test on a cycle ergometer (5 min warm-up at 30W, and subsequent steps of 3 min with power increases of 30W) under glycogen depletion by performing an exercise protocol to deplete glycogen stores plus a low-carbohydrate diet 24 h before incremental testing
Eligibility Criteria
You may qualify if:
- Perform endurance training with a volume of between 3 and 12h/week
- In women, having regular menstrual cycles (25-35 days length)
- In women, being able to perform the test without menstrual discomfort or pain and without having taken anti-inflammatory or analgesic drugs
You may not qualify if:
- Suffering from any previous disease, disorder or ailment that could affect the results of the study variables
- Smoking
- In women, using any type of hormonal contraceptive
- In women, having heavy menstrual bleeding, detected with the menstrual bleeding questionnaire proposed by Wyatt et al. (2001)
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Study Sites (1)
Laboratorio de Fisiología del Esfuerzo. Facultad de Ciencias de la Actividad Física y del Deporte (INEF)
Madrid, 28040, Spain
Related Publications (2)
Wyatt KM, Dimmock PW, Walker TJ, O'Brien PM. Determination of total menstrual blood loss. Fertil Steril. 2001 Jul;76(1):125-31. doi: 10.1016/s0015-0282(01)01847-7.
PMID: 11438330BACKGROUNDBenitez-Munoz JA, Guisado-Cuadrado I, Rojo-Tirado M, Alcocer-Ayuga M, Romero-Parra N, Peinado AB, Cupeiro R. Females have better metabolic flexibility in different metabolically challenging stimuli. Appl Physiol Nutr Metab. 2025 Jan 1;50:1-12. doi: 10.1139/apnm-2024-0217. Epub 2024 Oct 22.
PMID: 39437435DERIVED
MeSH Terms
Conditions
Interventions
Condition Hierarchy (Ancestors)
Intervention Hierarchy (Ancestors)
Study Officials
- PRINCIPAL INVESTIGATOR
Rocío Cupeiro, PhD
Universidad Politecnica de Madrid
Study Design
- Study Type
- interventional
- Phase
- not applicable
- Allocation
- RANDOMIZED
- Masking
- NONE
- Masking Details
- No Masking is possible since participants and investigators know which type of test (control, hyperthermia and glycogen depletion) is being performed.
- Purpose
- BASIC SCIENCE
- Intervention Model
- CROSSOVER
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Physical Activity and Sports PhD (Associate Professor)
Study Record Dates
First Submitted
January 11, 2023
First Posted
January 27, 2023
Study Start
January 28, 2023
Primary Completion
May 11, 2023
Study Completion
May 11, 2023
Last Updated
September 13, 2023
Record last verified: 2023-09