The Anabolic and Catabolic Effect of Loaded and Unloaded Exercise on Articular Cartilage Health

NCT05925244 | Completed

• 1 other identifier: HS22322
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

The serum concentration of cartilage oligomeric matrix protein (sCOMP) has been considered a mechano-sensitive biomarker of articular cartilage turnover, and N-propeptide of type II collagen (PIIANP) is a proposed biomarker of type II collagen synthesis. Few studies have investigated both the anabolic and turnover response of articular cartilage as a result of acute changes in body weight mass during exercise. Using a repeated measures cross-over design, fifteen healthy adults (age 18-30 years) performed three, 30-minute bouts of treadmill walking exercise under 3 loading conditions: (1) control (no alteration to body mass); (2) loaded (12% increase in body mass using a weighted vest); and (3) unloaded (12% decrease in body mass using lower body positive pressure). Venous blood was collected before, immediately after, and 15 and 30 minutes after exercise to investigate cartilage turnover (sCOMP) and anabolism (PIIANP).

Conditions

Healthy

Keywords

articular cartilage; walking exercise; sCOMP; PIIANP

Outcome Measures

Primary Outcomes (2)

• Serum cartilage oligomeric matrix protein (sCOMP)

  The systemic blood serum concentration of sCOMP was evaluated in all participants under the 3 conditions in a crossover trial.

  sCOMP was measured at baseline, immediately post, and 15- and 30-minutes post exercise.

• N-propeptide of type II collagen (PIIANP)

  The systemic blood serum concentration of PIIANP was evaluated in all participants under the 3 conditions in a crossover trial.

  PIIANP was measured at baseline, immediately post, and 15- and 30-minutes post exercise.

Study Arms (3)

Control

EXPERIMENTAL

The control condition did 30 minutes of walking exercise with their own body mass.

Other: Control Condition

+12% Body Mass

EXPERIMENTAL

The +12% body mass condition did 30 minutes of walking exercise wearing a weighted vest with +12% of their body mass applied.

Other: +12% Body Mass

-12% Body Mass

EXPERIMENTAL

The -12% body mass condition did 30 minutes of walking exercise using a lower body positive pressure treadmill with -12% of their body mass applied.

Other: -12% Body Mass

Interventions

Control Condition OTHER

Control (no alteration to body mass).

Control

-12% Body Mass OTHER

Unloaded walking exercise (12% decrease in body mass using lower body positive pressure).

-12% Body Mass

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy
• years old

You may not qualify if:

• Traumatic injury to the lower extremity in the past year
• Hip, knee, or ankle surgery
• Radiographic evidence of joint degeneration
• Cardiovascular disease
• Ankylosing spondylitis
• Psoriatic arthritis
• Chronic reactive arthritis
• Renal problems requiring peritoneal dialysis or hemodialysis
• Not currently using any prescription or non-prescription medications and/or nutritional supplements

Sponsors & Collaborators

• University of Manitoba: lead

Study Sites (1)

University of Manitoba

Winnipeg, Manitoba, R3T 2N2, Canada

Location

Related Publications (1)

• de Vasconcelos BM, Peeler JD, Scribbans T, Cornish SM. A preliminary study on the effect of loaded and unloaded exercise on N-propeptide of type II collagen and serum cartilage oligomeric matrix protein activity of articular cartilage in healthy young adults. Appl Physiol Nutr Metab. 2023 Dec 1;48(12):954-961. doi: 10.1139/apnm-2023-0124. Epub 2023 Aug 9.

  PMID: 37556859 DERIVED

Study Officials

• Stephen M Cornish, PhD

  University of Manitoba

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: June 21, 2023
• First Posted: June 29, 2023
• Study Start: September 27, 2019
• Primary Completion: March 1, 2020
• Study Completion: March 1, 2020
• Last Updated: July 3, 2023

Record last verified: 2023-06

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)