The Impact of Rapid Diagnostic Methods in the Diagnosis of Fungal Infections in Intensive Care Units of Assiut University Hospital

NCT05876962 | Unknown

• 1 other identifier: Diagnosis of fungal infection
• Study Type: observational
• Target: 100
• Locations: 0 countries
• Sites: N/A

Brief Summary

The diagnosis of invasive fungal disease remains challenging in the clinical laboratory. The use of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) for the identification of filamentous fungi as well as its application for antifungal resistance testing and strain typing Will be evaluated.

Conditions

Fungal Infection

Outcome Measures

Primary Outcomes (1)

• Phenotypically identify percentage of different fungal infections isolated from various clinical specimens in intensive care units. and Assess their antifungal susceptibility pattern.

  Assess the accuracy of Maldi-TOF in diagnosis of fungal infections

  baseline

Eligibility Criteria

• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients of all age group and both sex with clinically suspected fungal infection at intensive care units of Assiut university hospital will be included

You may qualify if:

• Patients of all age group and both sex with clinically suspected fungal infection at intensive care units of Assiut university hospital will be included.

You may not qualify if:

• Patients who are on antifungal treatment and refuse to take part will be excluded from the study.

Sponsors & Collaborators

• Assiut University: lead

Related Publications (5)

• Colombo AL, de Almeida Junior JN, Slavin MA, Chen SC, Sorrell TC. Candida and invasive mould diseases in non-neutropenic critically ill patients and patients with haematological cancer. Lancet Infect Dis. 2017 Nov;17(11):e344-e356. doi: 10.1016/S1473-3099(17)30304-3. Epub 2017 Jul 31.

  PMID: 28774702 BACKGROUND

• Pappas PG, Lionakis MS, Arendrup MC, Ostrosky-Zeichner L, Kullberg BJ. Invasive candidiasis. Nat Rev Dis Primers. 2018 May 11;4:18026. doi: 10.1038/nrdp.2018.26.

  PMID: 29749387 BACKGROUND

• Mayer FL, Wilson D, Hube B. Candida albicans pathogenicity mechanisms. Virulence. 2013 Feb 15;4(2):119-28. doi: 10.4161/viru.22913. Epub 2013 Jan 9.

  PMID: 23302789 BACKGROUND

• Neppelenbroek KH, Seo RS, Urban VM, Silva S, Dovigo LN, Jorge JH, Campanha NH. Identification of Candida species in the clinical laboratory: a review of conventional, commercial, and molecular techniques. Oral Dis. 2014 May;20(4):329-44. doi: 10.1111/odi.12123. Epub 2013 May 16.

  PMID: 23679136 BACKGROUND

• Vandeputte P, Ferrari S, Coste AT. Antifungal resistance and new strategies to control fungal infections. Int J Microbiol. 2012;2012:713687. doi: 10.1155/2012/713687. Epub 2011 Dec 1.

  PMID: 22187560 BACKGROUND

MeSH Terms

Conditions

Mycoses

Condition Hierarchy (Ancestors)

Bacterial Infections and Mycoses; Infections

Study Officials

• asmaa omar

  professor

  STUDY CHAIR

• mohamed zakaria

  professor

  STUDY CHAIR

Central Study Contacts

sara salah

CONTACT

01092127787; fawazsara91@gmail.com

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant lecturer

Study Record Dates

• First Submitted: May 17, 2023
• First Posted: May 26, 2023
• Study Start: January 1, 2024
• Primary Completion: May 1, 2024
• Study Completion: December 1, 2024
• Last Updated: January 30, 2024

Record last verified: 2023-07