Genes Variability in Obesity and Normal Body Weight Patients.

NCT05789888 | Completed

• 1 other identifier: SUB/3/DN/22/001/3316
• Study Type: observational
• Target: 116
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this clinical trial is to learn about variability of gene SCD and MT-ATP6 in describe participant population and health conditions. The main questions it aims to answer are: • whether there is variation in genes between obese and normal weight individuals Participants will get body weight and high was assessed using the medical scale and stadiometer, then, basis on obtained results, the Body Mass Index (BMI) will be calculated. Next, participants get determine the body composition and body composition analysis by bioelectrical impedance method to determined: Visceral Adipose Tissue (VAT in cm2 and %), Subcutaneous Adipose Tissue (SAT in cm2 and %), and the VAT/SAT ratio was also determined. Then order to examine the sequence of the genes, swab will take from the oral cavity (cheeks and palate) using sterile swab. The research was carried out at the Department of Dietetics and Clinical Nutrition (Medical University of Bialystok).

Conditions

Obesity, Visceral; Obesity

Keywords

SCD; MT-ATP6; Obesity; VAT; SAT; TOFI; FOTI; Genetic; Genes; Phenotype

Outcome Measures

Primary Outcomes (4)

• SCD variability between study and control group.

  Variability between groups will be determined based on the SCD gene sequence.

  1 day

• MT-ATP6 gene variability.

  Variability between groups will be determined based on the MT-ATP6 gene sequence.

  1 day

• Numbers of TOFI and FOTI phenotypes.

  On the basis of the VAT/SAT ratio, the abdominal adipose tissue phenotype will assessed.

  1 day

• Genetic variability between TOFI and FOTI phenotypes.

  Genetic variability between TOFI and FOTY phenotypes will be determined based on the SCD and MT-ATP6 genes.

  1 day

Study Arms (2)

Study group

The study group consisted of 75 people (46 women and 29 men) with gender (BMI=30.0-39.9 kg/m2).

Genetic: MT-ATP6 mtDNA gene variability in obesity and normal body weight patients.; Genetic: SCD gene variability in obesity and normal body weight patients.; Other: Finding TOFI and FOTI phenotypes in participants with obesity and normal weight.

Control group

The control group included 41 people (31 women and 10 men) with normal weight (BMI=18.5-24.9 kg/m2).

Genetic: MT-ATP6 mtDNA gene variability in obesity and normal body weight patients.; Genetic: SCD gene variability in obesity and normal body weight patients.; Other: Finding TOFI and FOTI phenotypes in participants with obesity and normal weight.

Interventions

MT-ATP6 mtDNA gene variability in obesity and normal body weight patients. GENETIC

As part of the research, the MT-ATP6 gene was tested in order to influence the modulation of the amount of fat contained.

Also known as: The search for obesity markers

Control group; Study group

SCD gene variability in obesity and normal body weight patients. GENETIC

As part of the research, the SCD gene was tested in order to influence the modulation of the amount of fat contained.

Also known as: The search for obesity markers

Control group; Study group

Finding TOFI and FOTI phenotypes in participants with obesity and normal weight. OTHER

As part of the research, we measured the amount of VAT and SAT and calculate VAT/SAT ratio as a marker to determine the phenotype of fat tissue.

Also known as: Phenotypes in obesity

Control group; Study group

Eligibility Criteria

• Age: 20 Years - 55 Years
• Sex: all
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Inhabitants of the Podlaskie Voivodeship.

You may qualify if:

• primary obesity and normal body weight

You may not qualify if:

• absence of inflammation and periodontal disease in the oral cavity
• secondary obesity
• type 1 diabetes
• type 2 diabetes
• endocrine disorders
• appetite disorders
• pregnancy and lactation
• acute coronary artery disease
• use of hormonal contraception or hormone replacement therapy
• steroid therapy
• antiretroviral therapy, previous surgical or pharmacological treatment for obesity and pacemaker

Sponsors & Collaborators

• Medical University of Bialystok: lead

Study Sites (1)

Medical University of Bialystok

Bialystok, Podlaskie Voivodeship, 15-089, Poland

Location

Biospecimen

Retention: SAMPLES WITH DNA

Swabs from mouth.

MeSH Terms

Conditions

Obesity, Abdominal; Obesity

Interventions

Adiposity; Phenotype

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Body Fat Distribution; Body Weights and Measures; Body Constitution; Physical Examination; Diagnostic Techniques and Procedures; Diagnosis; Body Composition; Biochemical Phenomena; Chemical Phenomena; Metabolism; Physiological Phenomena; Genetic Phenomena

Study Officials

• Damian D Pogodziński, Master

  Medical University of Białystok

  STUDY CHAIR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: OTHER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: March 8, 2023
• First Posted: March 29, 2023
• Study Start: April 10, 2021
• Primary Completion: January 1, 2023
• Study Completion: January 1, 2023
• Last Updated: March 29, 2023

Record last verified: 2023-02

Data Sharing

• IPD Sharing: Will not share

Locations

PL (1)