Distinct Sources of Supplementary Protein in the Resistance Exercise Training-induced Adaptations

NCT05710614 | Completed

• 1 other identifier: 66029522.3.0000.0068
• Study Type: interventional
• Target: 40
• Locations: 1 country
• Sites: 1

Brief Summary

This is a randomized controlled trial aimed to investigate the effects of different supplementary protein sources on muscle adaptations to resistance exercise. Young, healthy, recreationally active participants consuming an omnivorous diet with protein intake within recommended dietary allowance (RDA) (i.e.; 0.8-1.0 g.kg-1. d-1) will be recruited to undergo a 12-wk supervised resistance training program in combination with the intake of three 15-g daily doses (45g. d-1 total) of either a mixed plant- (i.e.; soy and pea protein) or animal-based (i.e.; whey protein) protein in drink form as a supplementary source of protein to their main meals of the day (i.e.; breakfast, lunch, and dinner). Before (PRE) and after (POST) the 12-wk intervention, participants will be assessed for body composition by dual-energy X-ray absorptiometry (DXA), muscle cross-sectional area (ultrasound) and fiber cross-sectional area (muscle biopsy), and maximal isotonic strength (1RM). Training consists of a resistance training (RT) program individually supervised by a researcher blinded to treatment in a laboratorial setting. Assessments will also be conducted in a blinded fashion.

Conditions

Dietary Supplements; Muscle Strength; Muscle, Skeletal; Resistance Training; Diet Habit

Outcome Measures

Primary Outcomes (1)

• vastus lateralis muscle cross sectional area

  the investigators used ultrasound measures of the vastus lateralis cross sectional area as the primary outcome

  Ultrasound images will be obtained both at baseline (before the comencement of the study) and immediately after the 12-week intervention period

Secondary Outcomes (3)

• lean mass

  DXA scans will be taken both at baseline (before the comencement of the study) and immediately after the 12-week intervention period

• fiber cross sectional area

  Fiber cross sectional areas will be measured both at baseline (before the comencement of the study) and immediately after the 12-week intervention period

• maximal isotonic strength

  1-RM tests will be taken both at baseline (before the comencement of the study) and immediately after the 12-week intervention period

Study Arms (2)

animal protein

ACTIVE COMPARATOR

Whey protein will be used as the active comparator for animal protein arm

Dietary Supplement: Animal protein

plant protein

EXPERIMENTAL

A combination of pea and soy protein will be used as the experimental arm

Dietary Supplement: Plant Protein

Interventions

Animal protein DIETARY_SUPPLEMENT

Patients allocated to this arm will be submitted a supervised resistance training program in combination with the intake of three 15-g daily doses (45g. d- 1 total) animal-based protein (i.e.; whey protein) in drink form as a supplementary source of protein in their main meals of the day.

animal protein

Plant Protein DIETARY_SUPPLEMENT

Patients allocated to this arm will be submitted a supervised resistance training program in combination with the intake of three 15-g daily doses (45g. d- 1 total) mixed plant protein (i.e.; soy and pea) in drink form as a supplementary source of protein in their main meals of the day.

plant protein

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Physically active (according to the International Physical Activity Questionnaire (IPAQ)
• Absence of any chronic condition that could preclude participation in a RT program or physical testing
• Habitual protein consumption within RDA ≥ 0.8 ≤1.0 g.kg-1. d-1

You may not qualify if:

• Prior history of anabolic steroids use, current or previous (≤ 3 m)
• Use of ergogenic or protein-based supplements, current or previous (\< 1 y)
• Engagement in energy-restricted diets

Sponsors & Collaborators

• University of Sao Paulo General Hospital: lead
• NotCo Brasil Distribuicao e Comercio de Produtos Alimenticios LTDA: collaborator

Study Sites (1)

University of Sao Paulo

São Paulo, 05508-030, Brazil

Location

Related Publications (1)

• Santini MH, Erwig Leitao A, Mazzolani BC, Smaira FI, de Souza MSC, Santamaria A, Gualano B, Roschel H. Similar effects between animal-based and plant-based protein blend as complementary dietary protein on muscle adaptations to resistance training: findings from a randomized clinical trial. J Int Soc Sports Nutr. 2025 Dec;22(1):2568047. doi: 10.1080/15502783.2025.2568047. Epub 2025 Oct 8.

  PMID: 41059835 DERIVED

MeSH Terms

Conditions

Feeding Behavior

Interventions

Plant Proteins

Condition Hierarchy (Ancestors)

Behavior, Animal; Behavior

Intervention Hierarchy (Ancestors)

Proteins; Amino Acids, Peptides, and Proteins

Study Officials

• Hamilton Roschel, PhD

  University of Sao Paulo

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Doctor of Philosophy

Study Record Dates

• First Submitted: January 20, 2023
• First Posted: February 2, 2023
• Study Start: August 1, 2023
• Primary Completion: November 27, 2024
• Study Completion: November 27, 2024
• Last Updated: December 6, 2024

Record last verified: 2024-12

Data Sharing

• IPD Sharing: Will not share

Locations

BR (1)