NCT05705986

Brief Summary

Microparticles (MPs) as a mode of therapeutic delivery can selectively deliver immunomodulatory treatment to the phagocytic cells, particularly dendritic cells (DCs), inducing their tolerogenic phenotype and function and T regulatory (Treg) cell expansion. The study will characterize the in vitro response of cGAMP immunomodulator incapsulated microparticles on the capacity of DCs and Tregs to regulate the inflammatory response.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
40

participants targeted

Target at P25-P50 for all trials

Timeline
Completed

Started Feb 2021

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

February 26, 2021

Completed
2 months until next milestone

First Submitted

Initial submission to the registry

April 20, 2021

Completed
1.8 years until next milestone

First Posted

Study publicly available on registry

January 31, 2023

Completed
1.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 1, 2024

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

May 1, 2024

Completed
Last Updated

January 31, 2023

Status Verified

January 1, 2023

Enrollment Period

3.2 years

First QC Date

April 20, 2021

Last Update Submit

January 20, 2023

Conditions

Outcome Measures

Primary Outcomes (2)

  • Characterize cGAMP MP-induced tolerogenic DCs from RRMS patients.

    Flow cytometry study will measure the cGAMP MP phagocytosis by PBMCs and characterize the phenotype of cGAMP MP-induced DCs of 40 untreated RRMS patients after incubation with fluorescently-labelled cGAMP MPs.

    Baseline

  • Determine the capacity of cGAMP MP-treated DCs to induce iTreg expansion in human in-vitro cultures

    Flow cytometry studies will characterize the induced Treg phenotype generated after co-culture with cGAMP MP-treated DCs cells to determine the capacity of the cGAMP MP-treated DCs to induce differentiation and expansion of iTregs

    Baseline

Secondary Outcomes (3)

  • Determine the transcriptome of cGAMP MP IL-27- and IL-10-induced Tregs.

    Baseline

  • Determine the in-vitro reconstitution of iTreg suppressive function in RRMS patients after coculture with cGAMP MP-treated DCs.

    Baseline

  • Induction of antigen-specific immune tolerance after co-administer DR2-aAPMs with the cGAMP MPs to DCs.

    Baseline

Interventions

Single blood draw

Eligibility Criteria

Age18 Years - 55 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64)
Sampling MethodNon-Probability Sample
Study Population

40 patients diagnosed with relapse remitting multiple sclerosis

You may qualify if:

  • Subjects meeting diagnostic criteria for relapsing remitting multiple sclerosis
  • Between the ages of 18-55
  • Not treated with immunomodulatory therapy, no other neurological diagnosis, no other inflammatory disease

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Thomas Jefferson University

Philadelphia, Pennsylvania, 19107, United States

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

Blood samples

MeSH Terms

Interventions

Blood Specimen Collection

Intervention Hierarchy (Ancestors)

Specimen HandlingClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisPuncturesSurgical Procedures, OperativeInvestigative Techniques

Central Study Contacts

Silva Markovic-Plese, MD, PhD

CONTACT

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

April 20, 2021

First Posted

January 31, 2023

Study Start

February 26, 2021

Primary Completion

May 1, 2024

Study Completion

May 1, 2024

Last Updated

January 31, 2023

Record last verified: 2023-01

Locations