NCT04857489

Brief Summary

The purpose of this research is to find out how the T regulatory (Treg) cells control autoimmune response in multiple sclerosis. The investigators will identify Treg molecular markers and changes in function in patients with relapse remitting multiple sclerosis (RRMS). The investigators plan to study T regulatory immune cells in the blood of RRMS patients and control subjects to examine how Treg immune cells' deficient function may be involved in the development of mulitple sclerosis.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
80

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Jun 2020

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 1, 2020

Completed
11 months until next milestone

First Submitted

Initial submission to the registry

April 20, 2021

Completed
3 days until next milestone

First Posted

Study publicly available on registry

April 23, 2021

Completed
2.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2023

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2023

Completed
Last Updated

June 15, 2022

Status Verified

June 1, 2022

Enrollment Period

3 years

First QC Date

April 20, 2021

Last Update Submit

June 13, 2022

Conditions

Outcome Measures

Primary Outcomes (2)

  • Characterize natural (n) Tregs' functional deficit in RRMS

    Functional suppression assays will compare Treg suppressive function in a third cohort of 10 HC and 10 RRMS patients.

    Baseline

  • Identify the role of inducible (i) Treg cells in maintaining the immunological tolerance in RRMS

    Baseline

Secondary Outcomes (6)

  • Identify the phenotype changes in CD4+CD25+CD127 nTregs in RRMS patients

    Baseline

  • Characterize functional deficits of nTregs in RRMS

    Baseline

  • Determine the effects of hemostatic cytokines on the nTregs' suppressive function in RRMS

    Baseline

  • Determine the phenotype of iTreg cells in RRMS

    Baseline

  • Identify the mechanisms of deficient nTreg induction of iTregs in RRMS

    Baseline

  • +1 more secondary outcomes

Study Arms (2)

Patients with Relapse Remitting Multiple Sclerosis

Blood sample

Healthy Controls

Blood Sample

Eligibility Criteria

Age18 Years - 55 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)
Sampling MethodNon-Probability Sample
Study Population

40 Patients with relapse remitting multiple sclerosis (RRMS), 40 Healthy Controls

You may qualify if:

  • Confirmed diagnosis of RRMS according to McDonald's diagnostic criteria
  • Age 18-55
  • Extended disability status score (EDSS) 1.5-5.5
  • No immunomodulatory therapy at the time of the study. (Treatment-free period will be at least 4 weeks for IV Methylprednisolone, Interferon-beta, Glatiramer acetate, Fingolimod, Tecfidera and Natalizumab. Patients previously treated with immunosuppressive therapies, including Azathioprine, Methotrexate, Mitoxantrone and Cyclophosphamide will not be enrolled in the study.)

You may not qualify if:

  • Concomitant infection
  • Significant medical and psychiatric condition at the discretion of principal investigator
  • Pregnant women
  • Patients participating in other research trials

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Thomas Jefferson University

Philadelphia, Pennsylvania, 19107, United States

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

Blood sample

Central Study Contacts

Silva Markovic-Plese, MD, PhD

CONTACT

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

April 20, 2021

First Posted

April 23, 2021

Study Start

June 1, 2020

Primary Completion

June 1, 2023

Study Completion

June 1, 2023

Last Updated

June 15, 2022

Record last verified: 2022-06

Locations