The Effect of Exercise on Myokine Production in Aging Persons

NCT05571709 | Unknown

• 1 other identifier: B1152022000006
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

The incidence of age-related diseases, such as cancer and cardiovascular diseases, rapidly rises. These days, age-related diseases cause the majority of all healthcare expenses. During ageing senescent cells secrete a range of harmful inflammatory signals, which leads to chronic inflammation (inflamm-aging) and accelerates aging throughout the rest of the body. Interestingly, people who regularly exercise show less signs of inflamm-aging compared to people with a sedentary lifestyle. Molecules secreted by skeletal muscles, called myokines, may play an important role. However, the underlying molecular mechanisms remain unknown. This pilot study aims to investigate if an acute bout of resistance training is an appropriate tool to induce an increase in the release of muscle derived myokines in aged persons.

Conditions

Aging; Exercise

Outcome Measures

Primary Outcomes (5)

• Cytokine concentration

  Measurement of multiple cytokines in blood (for example: IL-6, IL-15, IL-4, irisin...) with ELISA's or multiplex assays

  Day 2

• Myokine production

  Immunohistochemistry staining (fluorescent) of multiple myokines (for example: IL-6, IL-15, IL-4, irisin..) and aging markers (p16,p21...) on muscle tissue

  Day 2

• Immune parameters

  Flow cytometry panels of immune parameters in muscle tissue

  Day 2

• Myokine protein levels

  Western blotting of multiple myokines in muscle tissue

  Day 2

• Myokine gene expression

  Quantitative PCR (polymerase chain reaction) of markers in muscle tissue

  Day 2

Secondary Outcomes (3)

• Blood pressure

  Day 1

• Resting Heart Rate

  Day 1

• International Physical Activity Questionnaire (Short form)

  Day 1

Study Arms (2)

Aged individuals who perform exercise training

ACTIVE COMPARATOR

Aged individuals will perform an acute bout of resistance trainig (30 minutes). This training is focused on the lower limbs.

Other: Exercise training; Behavioral: Questionnaire; Procedure: Percutaneous muscle biopsy; Procedure: Blood sample

Aged individuals who stay sedentary

SHAM COMPARATOR

Aged individuals will remain seated for 30 minutes.

Behavioral: Questionnaire; Procedure: Percutaneous muscle biopsy; Procedure: Blood sample

Interventions

Exercise training OTHER

Resistance training of the lower limbs

Aged individuals who perform exercise training

Questionnaire BEHAVIORAL

Physical activity questionnaire

Aged individuals who perform exercise training; Aged individuals who stay sedentary

Percutaneous muscle biopsy PROCEDURE

Muscle biopsy of the vastus lateralis (m. quadriceps)

Aged individuals who perform exercise training; Aged individuals who stay sedentary

Blood sample PROCEDURE

Venous blood sample

Aged individuals who perform exercise training; Aged individuals who stay sedentary

Eligibility Criteria

• Age: 65 Years - 85 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Older Adult (65+)

You may qualify if:

• Healthy persons

You may not qualify if:

• Smoking
• Infection \< 2 weeks ago
• Received a vaccine \< 2 weeks ago
• Allergy to Lidocain
• Immune deficiency
• Presence of a tumour or in treatment
• Usage of anti-coagulant medication
• Presence of following conditions:
• Haemophilia
• Auto-immune disease
• Cardiovascular disease
• Musculoskeletal diseases which refrains the patient from exercising

Sponsors & Collaborators

• Hasselt University: lead

Study Sites (1)

Hasselt University

Diepenbeek, Limburg, 3590, Belgium

RECRUITING

MeSH Terms

Conditions

Motor Activity

Interventions

Exercise; Surveys and Questionnaires; Blood Specimen Collection

Condition Hierarchy (Ancestors)

Behavior

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena; Data Collection; Epidemiologic Methods; Investigative Techniques; Health Care Evaluation Mechanisms; Quality of Health Care; Health Care Quality, Access, and Evaluation; Public Health; Environment and Public Health; Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Surgical Procedures, Operative

Study Officials

• Chiel Hex, MD.

  University of Hasselt

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Bert Op'T Eijnde, Prof. dr.

CONTACT

+3211292121; bert.opteijnde@uhasselt.be

Chiel Hex, MD.

CONTACT

chiel.hex@uhasselt.be

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor Doctor

Model Details: Aged individuals who perform exercise training are compared to aged individuals who remain sedentary.

Study Record Dates

• First Submitted: October 5, 2022
• First Posted: October 7, 2022
• Study Start: September 15, 2022
• Primary Completion: October 15, 2023
• Study Completion: December 31, 2023
• Last Updated: March 31, 2023

Record last verified: 2023-03

Data Sharing

• IPD Sharing: Will not share

Locations

BE (1)