NCT05471076

Brief Summary

The clinical study is designed to evaluate the ability of two priming vaccine regimens to activate and induce the maturation of cross-reactive CD4 binding site (CD4-bs) antibodies, including VRC01-class antibodies. VRC01- class antibodies are highly desirable to elicit via vaccination because they have broad cover all clades of HIV and passive administration of VRC01 monoclonal antibodies has been demonstrated to prevent acquisition of susceptible HIV strains in clinical trials. The study will assess whether B cells expressing VRC01-like B cell receptors proliferate following immunization with a 'germline-targeting' recombinant Env immunogen. The study will also test whether an immunization strategy based upon fractionated dose delivery of the immunogen may improve the maturation of VRC01-class B cells when compared to traditional bolus dosing. In addition, the study will test whether alterations in the dose of the subsequent boost immunizations affects VRC01-class B cell activation and the rate of antibody affinity maturation. The primary hypothesis of the optional boost regimen is that BG505 SOSIP.GT1.1 gp140 adjuvated with 3M-052-AF + Alum is safe and well-tolerated and will further mature B-cell lineages elicited by 426c.Mod.Core-C4b priming regimens.

Trial Health

75
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
52

participants targeted

Target at P50-P75 for phase_1

Timeline
1mo left

Started Aug 2022

Typical duration for phase_1

Geographic Reach
1 country

6 active sites

Status
active not recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress99%
Aug 2022May 2026

First Submitted

Initial submission to the registry

July 15, 2022

Completed
7 days until next milestone

First Posted

Study publicly available on registry

July 22, 2022

Completed
1 month until next milestone

Study Start

First participant enrolled

August 22, 2022

Completed
3.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 23, 2025

Completed
5 months until next milestone

Study Completion

Last participant's last visit for all outcomes

May 21, 2026

Expected
Last Updated

April 13, 2026

Status Verified

April 1, 2026

Enrollment Period

3.3 years

First QC Date

July 15, 2022

Last Update Submit

April 9, 2026

Conditions

Outcome Measures

Primary Outcomes (8)

  • Number of participants showing local vaccination reactogenicity signs and symptoms

    Assessed by clinic staff. For a given sign or symptom, each subject's reactogenicity will be counted once under the maximum severity for each injection/ vaccination.

    14 days following each vaccination

  • Number of participants showing systemic vaccination reactogenicity signs and symptoms

    Assessed by clinic staff. For a given sign or symptom, each subject's reactogenicity will be counted once under the maximum severity for each injection/ vaccination.

    14 days following each vaccination

  • Number of serious adverse events (SAEs)

    Adverse events (AEs) will be graded according to the Division of AIDS (DAIDS) Table for Grading the Severity of Adult and Pediatric Adverse Events, Corrected Version 2.1 (exceptions apply).

    Through week 64

  • Number of medically attended adverse events (MAAEs)

    Adverse events (AEs) will be graded according to the Division of AIDS (DAIDS) Table for Grading the Severity of Adult and Pediatric Adverse Events, Corrected Version 2.1 (exceptions apply).

    Through week 64

  • Number of adverse events of special interest (AESIs)

    Adverse events (AEs) will be graded according to the Division of AIDS (DAIDS) Table for Grading the Severity of Adult and Pediatric Adverse Events, Corrected Version 2.1 (exceptions apply).

    Through week 64

  • Number of AEs leading to early participant withdrawal or permanent discontinuation

    Adverse events (AEs) will be graded according to the Division of AIDS (DAIDS) Table for Grading the Severity of Adult and Pediatric Adverse Events, Corrected Version 2.1 (exceptions apply).

    Through week 64

  • Frequency of CD4-bs-specific B cells

    Measured by flow cytometry analysis

    Through week 27

  • Frequency of VRC01-like BCR sequences

    Determined by variable heavy chain domain (VH)/variable light chain domain (VL) sequencing of sorted B cells

    Through week 27

Secondary Outcomes (22)

  • Response rate of Env-specific serum IgG binding antibodies.

    Through week 27

  • Magnitude of Env-specific serum IgG binding antibodies.

    Through week 27

  • Epitope-specificity of Env-specific serum IgG binding antibodies.

    Through week 27

  • Avidity of Env-specific serum IgG binding antibodies.

    Through week 27

  • Frequency of CD4-bs B cells and BCR sequences of isolated CD4-bs and VRC01-class memory B cells induced post-immunization with the traditional bolus dosing versus the fractionated dose delivery approach.

    Through week 27

  • +17 more secondary outcomes

Study Arms (7)

Bolus Delivery, Group 1: First injection - Medium dose, Final injection - Lower dose

EXPERIMENTAL

The Bolus Delivery Arm Group 1 will receive injections at 2 visits scheduled 3 months apart. At each injection visit the patient will get one dose divided into 2 injections - one in the deltoid muscle of each arm.

Biological: 426c.Mod.Core-C4b 30 mcgBiological: 426c.Mod.Core-C4b 100 mcg

Bolus Delivery, Group 2: First injection - Medium dose, Final injection - Higher dose

EXPERIMENTAL

The Bolus Delivery Arm Group 2 will receive injections at 2 visits scheduled 3 months apart. At each injection visit the patient will get one dose divided into 2 injections - one in the deltoid muscle of each arm.

Biological: 426c.Mod.Core-C4b 100 mcgBiological: 426c.Mod.Core-C4b 300 mcg

Bolus Delivery, Group 3: First injection - Placebo, Final injection - Placebo

PLACEBO COMPARATOR

The Bolus Delivery Arm Group 2 will receive injections at 2 visits scheduled 3 months apart. At each injection visit the patient will get one dose divided into 2 injections - one in the deltoid muscle of each arm.

Other: Placebo

Fractionated Delivery, Group 1: First injection - Medium dose, Final injection - Lower dose

EXPERIMENTAL

The first dose for the Fractionated Delivery Arm Group 1 will be divided into 6 smaller amounts (2 visits per week for 3 weeks). The second dose will be given about 3 months later and will be given all at once. At each injection visit for the first dose, the patient will get one amount divided into 2 injections - one in the deltoid muscle of each arm.

Biological: 426c.Mod.Core-C4b 30 mcgBiological: 426c.Mod.Core-C4b 100 mcg

Fractionated Delivery, Group 2: First injection - Medium dose, Final injection - Higher dose

EXPERIMENTAL

The first dose for the Fractionated Delivery Arm Group 2 will be divided into 6 smaller amounts (2 visits per week for 3 weeks). The second dose will be given about 3 months later and will be given all at once. At each injection visit for the first dose, the patient will get one amount divided into 2 injections - one in the deltoid muscle of each arm.

Biological: 426c.Mod.Core-C4b 100 mcgBiological: 426c.Mod.Core-C4b 300 mcg

Fractionated Delivery, Group 3: First injection - Placebo, Final injection - Placebo

PLACEBO COMPARATOR

The first dose for the Fractionated Delivery Arm Group 2 will be divided into 6 smaller amounts (2 visits per week for 3 weeks). The second dose will be given about 3 months later and will be given all at once. At each injection visit for the first dose, the patient will get one amount divided into 2 injections - one in the deltoid muscle of each arm.

Other: Placebo

Optional Boost Regimen with BG505

EXPERIMENTAL

SOSIP.GT1.1 gp140

Biological: BG505

Interventions

Administered via injection as a split dose into the deltoid muscle (both left and right).

Also known as: Medium dose
Bolus Delivery, Group 1: First injection - Medium dose, Final injection - Lower doseBolus Delivery, Group 2: First injection - Medium dose, Final injection - Higher doseFractionated Delivery, Group 1: First injection - Medium dose, Final injection - Lower doseFractionated Delivery, Group 2: First injection - Medium dose, Final injection - Higher dose

Administered via injection as a split dose into the deltoid muscle (both left and right).

Also known as: Higher dose
Bolus Delivery, Group 2: First injection - Medium dose, Final injection - Higher doseFractionated Delivery, Group 2: First injection - Medium dose, Final injection - Higher dose
PlaceboOTHER

Administered via injection as a split dose into the deltoid muscle (both left and right).

Bolus Delivery, Group 3: First injection - Placebo, Final injection - PlaceboFractionated Delivery, Group 3: First injection - Placebo, Final injection - Placebo
BG505BIOLOGICAL

A soluble, cleavage-competent, trimeric HIV-1 envelope glycoprotein gp140 formulated at 2 mg/mL, 0.55 mL per vial, in 20 mM Tris, 100 mM NaCl, pH 7.5 will be admixed with 3M-052-AF (5 mcg) + Alum (500 mcg

Optional Boost Regimen with BG505

Administered via injection as a split dose into the deltoid muscle (both left and right).

Also known as: Lower dose
Bolus Delivery, Group 1: First injection - Medium dose, Final injection - Lower doseFractionated Delivery, Group 1: First injection - Medium dose, Final injection - Lower dose

Eligibility Criteria

Age18 Years - 55 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Able and willing to complete the informed consent process, including an Assessment of Understanding: volunteer demonstrates understanding of this study; completes a questionnaire prior to first vaccination with verbal demonstration of understanding of any questionnaire items answered incorrectly.
  • years old, inclusive, on day of enrollment.
  • Available for clinic follow-up through the last clinic visit, willing to under go lymph node fine needle aspiration, and willing to be contacted 12 months after the last vaccine administration.
  • Agrees not to enroll in another study of an investigational agent during participation in the trial.
  • In good general health according to the clinical judgement of the site investigator.
  • Physical examination and laboratory results without clinically significant findings that would interfere with assessment of safety or reactogenicity in the clinical judgement of the site investigator.
  • Assessed as low risk for HIV acquisition per low risk guidelines, agrees to discuss HIV infection risks, agrees to risk reduction counseling, and agrees to avoid behavior associated with high risk of HIV exposure through the final study visit. Low risk may include persons stably taking pre-exposure prophylaxis (PrEP) as prescribed for 6 months or longer.
  • Hemoglobin:
  • ≥ 11.0 g/dL for volunteers who were assigned female sex at birth
  • ≥ 13.0 g/dL for volunteers who were assigned male sex at birth and transgender males who have been on hormone therapy for more than 6 consecutive months
  • ≥ 12.0 g/dL for transgender females who have been on hormone therapy for more than 6 consecutive months
  • For transgender participants who have been on hormone therapy for less than 6 consecutive months, determine hemoglobin eligibility based on the sex assigned at birth.
  • White blood cell (WBC) count \> 3,500/mm3.
  • Platelets ≥125,000/mm3.
  • Alanine aminotransferase (ALT) \< 2.5 x upper limit of normal (ULN) based on the institutional normal range.
  • +8 more criteria

You may not qualify if:

  • Volunteer who is breast-feeding or pregnant.
  • Morbid Obesity. Enrollment of individuals with body mass index (BMI) ≥40, who the site investigator assesses are in good health, may be considered by PSRT on a case-by-case basis.
  • International normalized ratio (INR) \>1.2.
  • Previous or current recipient of an investigational HIV vaccine (previous placebo recipients are not excluded).
  • Receipt of non-HIV experimental vaccine(s) received within the last 1 year. Exceptions may be made by the PSRT for vaccines that have subsequently undergone licensure or Emergency Use Authorization by the FDA or, if outside the United States, by the national regulatory authority or World Health Organization. For volunteers who have received control/placebo in an experimental vaccine trial, the PSRT will determine eligibility on a case-by-case basis.
  • Systemic glucocorticoid use equal to or greater than prednisone 10 mg/day within 3 months prior to enrollment, other systemic medication use likely to impair immune response to vaccine in the opinion of the site investigator, or congenital or acquired immunodeficiency.
  • Blood products or immunoglobulin within 16 weeks prior to enrollment; receipt of immunoglobulin within 16 weeks prior to enrollment requires PSRT approval.
  • Previous receipt of VRC01 monoclonal antibody.
  • Receipt of any live replicating vaccine within 4 weeks prior to enrollment. For receipt of ACAM2000 vaccine \>28 days prior with a vaccination scab still present.
  • Receipt of investigational research agents with a half-life of 7 or fewer days within 4 weeks prior to enrollment. If a potential participant has received investigational agents with a half-life greater than 7 days (or unknown half-life) within the past year, PSRT approval is required for enrollment.
  • History of serious reaction (eg, hypersensitivity, anaphylaxis) to any vaccine or any component of the study vaccine, including imidazoquinolone (eg, imiquimod).
  • Hereditary angioedema, acquired angioedema, or idiopathic forms of angioedema.
  • Idiopathic urticaria within the past year.
  • Bleeding disorder diagnosed by a doctor (eg, factor deficiency, coagulopathy, or platelet disorder requiring special precautions).
  • Asplenia or functional asplenia.
  • +8 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (6)

Alabama CRS

Birmingham, Alabama, 35222, United States

Location

Bridge HIV CRS

San Francisco, California, 94102, United States

Location

The Ponce de Leon Center CRS

Atlanta, Georgia, 30308-2012, United States

Location

Brigham and Women's Hospital Vaccine CRS (BWH VCRS)

Boston, Massachusetts, 02115-6110, United States

Location

Columbia P&S CRS

New York, New York, 10032, United States

Location

Seattle Vaccine and Prevention CRS

Seattle, Washington, 98104, United States

Location

Related Publications (1)

  • Libera M, Caputo V, Laterza G, Moudoud L, Soggiu A, Bonizzi L, Diotti RA. The Question of HIV Vaccine: Why Is a Solution Not Yet Available? J Immunol Res. 2024 Apr 8;2024:2147912. doi: 10.1155/2024/2147912. eCollection 2024.

Study Officials

  • Hyman Scott, MD

    University of California, San Francisco

    STUDY CHAIR
  • Kristen Cohen, MD

    Fred Hutch Cancer Center, Seattle, WA

    STUDY CHAIR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Masking Details
Whether a participant receives a bolus dose or fractionated dose will be known, but randomization will occur within a given arm and both participants and clinicians will be blinded within a given arm.
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
NIH
Responsible Party
SPONSOR

Study Record Dates

First Submitted

July 15, 2022

First Posted

July 22, 2022

Study Start

August 22, 2022

Primary Completion

December 23, 2025

Study Completion (Estimated)

May 21, 2026

Last Updated

April 13, 2026

Record last verified: 2026-04

Data Sharing

IPD Sharing
Will not share

Locations