NCT05306067

Brief Summary

Mozambique is among the ten countries with the highest burden of malaria worldwide, with an estimated 9.3 million cases in 2018, and constitutes a core target for the World Health Organization (WHO) and the Roll Back Malaria Partnership to End Malaria's country-led 'high burden to high impact' initiative. At the same time, the National Malaria Control Program (NMCP) of Mozambique seeks to accelerate elimination in the south, where transmission is lowest. NMCP is currently working with partners (Malaria Consortium, PMI, Global Fund) to set up a high-resolution surveillance system that can drive decision-making across all transmission strata through strengthening of routine data quality, data use and data to action packages. However, decisions become more complex as control reveals heterogeneity and better tools are required for a strategic use of information to drive impact. The overall objective of the study is to operationalize a functional malaria molecular surveillance (MMS) system that generates reliable and reproducible genomic data over time for programmatic decisions. The integration of genomic data into routine surveillance activities has the potential to increase the actionable intelligence for making programmatic decisions on the optimal mix of control and elimination measures in Mozambique by:

  1. 1.Informing drug and diagnostic choices through the monitoring of antimalarial drug resistance and diagnostic resistance (hrp2/3 deletions);
  2. 2.Targeting the reservoirs sustaining transmission through the use of transmission network models to quantify parasite importation, identify sources and characterize local transmission in near-elimination settings;
  3. 3.Improving stratification, monitoring and impact evaluations in different epidemiological and health system contexts through the use of measures of P. falciparum genetic diversity (routinely from positive cases) to supplement traditional surveillance, especially where it is sparse;
  4. 4.Using alternative, cost-effective, approaches targeting easy-access populations (e.g. pregnant women at antenatal care clinics) to monitor transmission and antimalarial/diagnostic resistance.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
21,848

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Oct 2021

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

October 12, 2021

Completed
5 months until next milestone

First Submitted

Initial submission to the registry

March 15, 2022

Completed
16 days until next milestone

First Posted

Study publicly available on registry

March 31, 2022

Completed
1.8 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2023

Completed
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

March 31, 2024

Completed
Last Updated

August 27, 2024

Status Verified

August 1, 2024

Enrollment Period

2.2 years

First QC Date

March 15, 2022

Last Update Submit

August 26, 2024

Conditions

Keywords

drug resistancediagnostic resistancesurveillancegenomicsimportationgenetic diversityplasmodium falciparumnext generation sequencingstratification

Outcome Measures

Primary Outcomes (3)

  • Prevalence of molecular markers of diagnostic resistance and antimalarial drug resistance by period, study area and population

    Year 3

  • Genetic diversity of the parasite population by period, study area and population

    Year 3

  • Genetic relatedness between pairs of samples and populations by period, study area and population

    Year 3

Study Arms (4)

Children at low transmission health facilities

Children at medium-to-high transmission health facilities

Diagnostic Test: LDH-based malaria rapid diagnostic test

Pregnant women at low transmission ANC

Diagnostic Test: Malaria rapid diagnostic test

Pregnant women at medium-to-high transmission transmission ANC

Diagnostic Test: Malaria rapid diagnostic test

Interventions

Malaria testing using an LDH-based malaria rapid diagnostic test will be added to standard routine testing of suspected cases at health facilities

Children at medium-to-high transmission health facilities

All pregnant women attending their first routine antenatal visit will be tested for malaria using routine malaria rapid diagnostic tests

Pregnant women at low transmission ANCPregnant women at medium-to-high transmission transmission ANC

Eligibility Criteria

Age6 Months+
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

In low transmission areas, people of any age (\> 6 months of age), with clinical symptoms of malaria and a parasitologically confirmed malaria diagnosis via RDT or microscopy will be invited to participate in the study, along with any household contacts of these malaria index cases, if they test P. falciparum positive with an RDT. Malaria-positive index cases of all ages from Magude and Matutuine will be also recruited, as well as individuals crossing the Matutuine Ponta d'Ouro border post who have a malaria positive RDT. In medium-to-high transmission areas, children under 2-10 years old, with clinical symptoms of malaria and a P. falciparum positive RDT will be invited to participate. Pregnant women attending their first antenatal care visit (any trimester) will be invited to participate in both trasnmission areas.

You may qualify if:

  • Any age
  • Fever (axillary temperature ≥37.5ºC) or history of fever in the preceding 24 hours
  • Positive parasitological test for malaria diagnosis via RDT or microscopy OR
  • Household contact of someone with fever/history of fever and Pf positive RDT
  • Positive parasitological test for malaria diagnosis via RDT OR
  • Pregnant women attending first antenatal care visit AND
  • Informed, written consent to participate from participant and/or guardian

You may not qualify if:

  • Any symptoms of severe malaria
  • Negative parasitological test for malaria via RDT or microscopy (except any women at their first ANC visit, who will be recruited before testing for malaria with an RDT)
  • Unwilling to provide informed, written consent
  • History of antimalarial treatment in the last 14 days
  • B) MEDIUM-TO-HIGH TRANSMISSION AREAS
  • Children 2-10 years of age
  • Fever (axillary temperature ≥37.5ºC) or history of fever in the preceding 24 hours
  • Positive parasitological test for malaria diagnosis via RDT or microscopy OR
  • Pregnant women attending first antenatal care visit AND
  • Informed, written consent to participate from participant and/or guardian
  • Any symptoms of severe malaria
  • Negative parasitological test for malaria via RDT or microscopy (except any women at their first ANC visit, who will be recruited before testing for malaria with an RDT)
  • Unwilling to provide informed, written consent
  • History of antimalarial treatment in the last 14 days

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Centro de Investigaçao em Saúde de Manhiça

Manhiça, Maputo Province, 1929, Mozambique

Location

Related Publications (1)

  • Mayor A, da Silva C, Rovira-Vallbona E, Roca-Feltrer A, Bonnington C, Wharton-Smith A, Greenhouse B, Bever C, Chidimatembue A, Guinovart C, Proctor JL, Rodrigues M, Canana N, Arnaldo P, Boene S, Aide P, Enosse S, Saute F, Candrinho B. Prospective surveillance study to detect antimalarial drug resistance, gene deletions of diagnostic relevance and genetic diversity of Plasmodium falciparum in Mozambique: protocol. BMJ Open. 2022 Jul 12;12(7):e063456. doi: 10.1136/bmjopen-2022-063456.

Related Links

Biospecimen

Retention: SAMPLES WITH DNA

Dried blood spot onto filter paper and rapid diagnostic tests collected by finger-prick.

MeSH Terms

Conditions

Malaria, FalciparumMalaria

Condition Hierarchy (Ancestors)

Protozoan InfectionsParasitic DiseasesInfectionsMosquito-Borne DiseasesVector Borne Diseases

Study Design

Study Type
observational
Observational Model
CASE ONLY
Time Perspective
CROSS SECTIONAL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

March 15, 2022

First Posted

March 31, 2022

Study Start

October 12, 2021

Primary Completion

December 31, 2023

Study Completion

March 31, 2024

Last Updated

August 27, 2024

Record last verified: 2024-08

Locations