Effect of a 4-week Post-exercise Sauna Bathing on Targeted Gut Microbiota

NCT05277597 | Completed

• 1 other identifier: PoznanUPhyEd 25.02.2020
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

Body temperature fluctuations induced by acute exercise bouts may influence the intestinal barrier with related effects on epithelial permeability, immune responses, and release of metabolites produced by the gut microbiota.

Conditions

Healthy Males

Keywords

gut microbiota, exercise, sauna bathing, intestinal barrier

Outcome Measures

Primary Outcomes (3)

• Change from abundance of Faecalibacterium prausnitzi of the genus Faecalibacterium, Akkermansia muciniphila of the genus Akkermansia, Bifidobacterium spp. of the genus Actinobacteria and Bacteroides spp. of the genus Bacteroidetes at 4 weeks.

  Bacterial DNA was isolated from stool samples using the QIAamp Fast DNA Stool Mini Kit (QIAGEN, Danish). The anaerobic bacteria were determined by Real-Time PCR with appropriate primers (ThermoFisher Scientific, USA).The results of quantitative bacterial analysis were converted to the decimal logarithm (Log10). The entire Real-Time PCR methodology was developed and validated by the Institute of Microecology in Herborn, Germany

  baseline and immediately after the intervention

• Change from the concentrations of sIgA (marker of mucosal immunity), and zonulin (marker of intestinal permeability) in stool at 4 weeks.

  Secretory immunoglobulin A concentrations in stool samples were determined with the Secretory IgA test (ImmuChrom GmbH, Heppenheim, Germany). Zonulin concentrations were assessed using the IKD Zonulin ELISA Kit (Immunodiagnostik AG, Bensheim, Germany).

  baseline and immediately after the intervention

• Change from the concentration of high-sensitivity C-reactive protein (hsCRP) at 4 weeks.

  The concentration of hsCRP was measured by immunoenzymatic assay using a commercially available kit (DRG International Inc., Springfield Township, NJ, USA).

  baseline and immediately after the intervention

Secondary Outcomes (2)

• Change from the white blood cell counts (WBC) and subsets at 4 weeks.

  baseline and immediately after the intervention

• Change feom cardio-respiratory measures at 4 weeks.

  baseline and immediately after the intervention

Study Arms (2)

The group (ET+S )

EXPERIMENTAL

Participants exercised 60 minutes, 3 times per week, on a bicycle ergometer followed by a 30-minute dry Finish sauna treatment.

Diagnostic Test: The exercise 60 minutes, 3 times per week, on a bicycle ergometer followed by a 30-minute dry Finish sauna treatment.

The group (ET)

ACTIVE COMPARATOR

Participants exercised 60 minutes, 3 times per week

Diagnostic Test: The same exercise training program without the sauna treatments

Interventions

The exercise 60 minutes, 3 times per week, on a bicycle ergometer followed by a 30-minute dry Finish sauna treatment. DIAGNOSTIC_TEST

Participants in the group ET+S (n=8) exercised 60 minutes, 3 times per week, on a bicycle ergometer followed by a 30-minute dry Finish sauna treatment.Blood and stool samples were collected before and after the 4-week training program.

Also known as: ET+S

The group (ET+S )

The same exercise training program without the sauna treatments DIAGNOSTIC_TEST

The control group (ET, n=7) engaged in the same exercise training program without the sauna treatments. Blood and stool samples were collected before and after the 4-week training program.

Also known as: ET

The group (ET)

Eligibility Criteria

• Age: 18 Years - 25 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• absence of medical contraindications such as epilepsy,addiction to medicines, alcohol and drugs, cancer, blood clotting disorders,
• no infections in the last 4 weeks prior to the study,
• no injuries in the last 4 weeks prior to the study.

You may not qualify if:

• the intake of antibiotics, steroids, oral antifungal agents (except for topical antifungals), antiparasitic agents, pre- and/or probiotics,
• history of travel to tropical countries during the last 4 weeks before the study,
• history of adverse responses to sauna bathing.

Sponsors & Collaborators

• Poznan University of Physical Education: lead
• David C. Nieman: collaborator
• Tomasz Cisoń: collaborator
• Joanna Szurkowska: collaborator
• Mirosława Gałęcka: collaborator
• Dariusz Sitkowski: collaborator
• Zbigniew Szyguła: collaborator

Study Sites (1)

Tomasz Cisoń

Nowy Sącz, 33-300, Poland

Location

Related Publications (1)

• Karolkiewicz J, Nieman DC, Cison T, Szurkowska J, Galecka M, Sitkowski D, Szygula Z. No effects of a 4-week post-exercise sauna bathing on targeted gut microbiota and intestinal barrier function, and hsCRP in healthy men: a pilot randomized controlled trial. BMC Sports Sci Med Rehabil. 2022 Jun 16;14(1):107. doi: 10.1186/s13102-022-00497-z.

  PMID: 35710395 DERIVED

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Joanna Karolkiewicz, Prof.

  Poznan University of Physical Education

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: DIAGNOSTIC
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: prof. AWF dr hab.

Model Details: This study used a randomized, parallel group design. The participants were randomly assigned to exercise training (ET) without or with post-exercise sauna treatments (ET+S) by using a permuted blocks of block size 4. design with a computer.

Study Record Dates

• First Submitted: February 28, 2022
• First Posted: March 14, 2022
• Study Start: February 10, 2020
• Primary Completion: March 20, 2020
• Study Completion: June 25, 2021
• Last Updated: March 14, 2022

Record last verified: 2022-03

Data Sharing

• IPD Sharing: Will not share

Locations

PL (1)