Metabolic Profiling of Hematopoietic Stem Cells in Clonal Hematopoiesis (CHIP)

NCT05246813 | Recruiting

• 1 other identifier: 20211214
• Study Type: observational
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

Bone marrow samples will be collected from patients undergoing hip arthroplasty surgery. Blood and bone marrow samples will be used for metabolic profiling and analysis of relevant CHIP mutations. Combined single-cell transcriptomics and mutation-specific single-cell genotyping (biotin-PCR using mutation-targeted primers followed by sequencing) will subsequently be performed. The gene expression profile of wildtype and mutant hematopoietic stem cells will be compared, performing both broad gene set enrichment analysis and targeted analysis of metabolic pathways.

Conditions

Osteoporosis; Osteoarthritis, Hip; Hip Fractures; Aging; Clonal Hematopoiesis of Indeterminate Potential

Outcome Measures

Primary Outcomes (1)

• Gene Set Enrichment Analysis (GSEA) Normalized Enrichment Score (NES)

  One day

Study Arms (2)

Osteoporotic hip fracture patients

Other: Blood and bone marrow cell collection

Hip osteoarthritis patients

Other: Blood and bone marrow cell collection

Interventions

Blood and bone marrow cell collection OTHER

Collection of blood and bone marrow

Hip osteoarthritis patients; Osteoporotic hip fracture patients

Eligibility Criteria

• Age: 55 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients undergoing hip arthroplasty for hip osteoarthritis or osteoporotic hip fractures.

You may qualify if:

• Age 65 years and older

You may not qualify if:

• known haematological condition (myelodysplasia, leukemia, cancer)
• inability to provide written informed consent

Sponsors & Collaborators

• Université Catholique de Louvain: collaborator
• Imelda Hospital, Bonheiden: lead

Study Sites (1)

Imelda Hospital

Bonheiden, België, 2820, Belgium

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

EDTA blood and bone marrow samples

MeSH Terms

Conditions

Osteoporosis; Osteoarthritis, Hip; Hip Fractures

Interventions

Blood Specimen Collection

Condition Hierarchy (Ancestors)

Bone Diseases, Metabolic; Bone Diseases; Musculoskeletal Diseases; Metabolic Diseases; Nutritional and Metabolic Diseases; Osteoarthritis; Arthritis; Joint Diseases; Rheumatic Diseases; Femoral Fractures; Fractures, Bone; Wounds and Injuries; Hip Injuries; Leg Injuries

Intervention Hierarchy (Ancestors)

Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Surgical Procedures, Operative; Investigative Techniques

Study Officials

• Nick Van Gastel, PhD

  de Duve Institute

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Michaël R. Laurent, MD PhD

CONTACT

+32485143267; michael.laurent@imelda.be

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: February 10, 2022
• First Posted: February 18, 2022
• Study Start: February 11, 2022
• Primary Completion (Estimated): December 31, 2028
• Study Completion (Estimated): December 31, 2030
• Last Updated: March 25, 2026

Record last verified: 2026-03

Locations

BE (1)