Exploring the Relationship Between the Gut Microbiome, Physical Fitness Levels and Metabolic Responses to Exercise

NCT05220657 | Completed DMC

• 1 other identifier: 2021-A02496-35
• Study Type: observational
• Target: 50
• Locations: 1 country
• Sites: 1

Brief Summary

Gut microbiota are all microorganisms including bacteria and microscopic eukaryotes that live in the digestive tracts of humans or mammals. During the last decade, some authors highlighted that a link exists between gut microbiota and sport performance. In this project, we hypothesize that gut microbiota is able to adapt to the energy needs of the body, really higher in top-level athletes or considerably lower in inactive individuals. In this context, this clinical study aims to characterize the bacterial metagenome of gut microbiota from populations located in a continuum from sedentary people to top-level athletes with high (i.e. soccer players), even very high energy needs (i.e. cyclists). The finality of this project is thus to determine if it exists some bacterial profile allowing to characterize, even to predict, the energy metabolism of an athlete and so the probability to be performant in competition.

Conditions

Elite Cyclists; Elite Soccer Players; Sedentary Behavior

Keywords

Physical activity; Carbohydrates and lipid metabolisms; Gut microbiota; Maximal oxygen consumption

Outcome Measures

Primary Outcomes (1)

• Gut microbiota composition

  Whole metagenomic sequencing using shotgun approach

  week 1

Secondary Outcomes (6)

• Gut microbiota function

  Week 1

• Short chain fatty acids levels in stools

  week 1

• Amino acids levels in stools

  week 1

• Maximal oxygen consumption (VO2max)

  Week 2

• Lipid oxidation during physical exercise

  week 3

Study Arms (4)

Low active subjects

No intervention

Diagnostic Test: Maximal incremental exercise test; Diagnostic Test: Submaximal exercise test

Moderately active subjects

No intervention

Diagnostic Test: Maximal incremental exercise test; Diagnostic Test: Submaximal exercise test

Elite soccer players

No intervention

Diagnostic Test: Maximal incremental exercise test; Diagnostic Test: Submaximal exercise test

Elite cyclists

No intervention

Diagnostic Test: Maximal incremental exercise test; Diagnostic Test: Submaximal exercise test

Interventions

Maximal incremental exercise test DIAGNOSTIC_TEST

Gas exchanges are measured during all the test on ergocycle until oxygen consumption reach its maximum value

Elite cyclists; Elite soccer players; Low active subjects; Moderately active subjects

Submaximal exercise test DIAGNOSTIC_TEST

A 25-min submaximal exercise test on ergocycle under fasting condition. Gas exchanges are measured during all the test.

Elite cyclists; Elite soccer players; Low active subjects; Moderately active subjects

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Populations located in a continuum from inactive people to top-level athletes with high (i.e. soccer players) and very high energy needs (i.e. cyclists).

You may qualify if:

• BMI between 18 and 25 kg/m²
• Non-smoker
• Written informed consent

You may not qualify if:

• Cardiovascular risks
• Metabolic diseases (e.g. diabetes)
• Use of antibiotics, anti-fungi or anti-parasites in the last 3 months or during participation in the study
• Use of prebiotics and / or probiotics in the form of supplements in the 7 days preceding the start of the study (greater than or equal to 100000000 Colony Forming Units or organisms per day)
• Taking drug treatment for chronic pain management (paracetamol, vasodilator, homeopathy, aspirin greater than 500 mg per day)

Sponsors & Collaborators

• University of Rennes 2: lead
• Nahibu: collaborator

Study Sites (1)

University Rennes 2 - Laboratory "Movement, Sport and health Sciences"

Bruz, Brittany Region, 35170, France

Location

Related Publications (5)

• Nay K, Jollet M, Goustard B, Baati N, Vernus B, Pontones M, Lefeuvre-Orfila L, Bendavid C, Rue O, Mariadassou M, Bonnieu A, Ollendorff V, Lepage P, Derbre F, Koechlin-Ramonatxo C. Gut bacteria are critical for optimal muscle function: a potential link with glucose homeostasis. Am J Physiol Endocrinol Metab. 2019 Jul 1;317(1):E158-E171. doi: 10.1152/ajpendo.00521.2018. Epub 2019 Apr 30.

  PMID: 31039010 BACKGROUND

• Clarke SF, Murphy EF, O'Sullivan O, Lucey AJ, Humphreys M, Hogan A, Hayes P, O'Reilly M, Jeffery IB, Wood-Martin R, Kerins DM, Quigley E, Ross RP, O'Toole PW, Molloy MG, Falvey E, Shanahan F, Cotter PD. Exercise and associated dietary extremes impact on gut microbial diversity. Gut. 2014 Dec;63(12):1913-20. doi: 10.1136/gutjnl-2013-306541. Epub 2014 Jun 9.

  PMID: 25021423 BACKGROUND

• Barton W, Penney NC, Cronin O, Garcia-Perez I, Molloy MG, Holmes E, Shanahan F, Cotter PD, O'Sullivan O. The microbiome of professional athletes differs from that of more sedentary subjects in composition and particularly at the functional metabolic level. Gut. 2018 Apr;67(4):625-633. doi: 10.1136/gutjnl-2016-313627. Epub 2017 Mar 30.

  PMID: 28360096 BACKGROUND

• Voorhies AA, Mark Ott C, Mehta S, Pierson DL, Crucian BE, Feiveson A, Oubre CM, Torralba M, Moncera K, Zhang Y, Zurek E, Lorenzi HA. Study of the impact of long-duration space missions at the International Space Station on the astronaut microbiome. Sci Rep. 2019 Jul 9;9(1):9911. doi: 10.1038/s41598-019-46303-8.

  PMID: 31289321 BACKGROUND

• Allen JM, Mailing LJ, Niemiro GM, Moore R, Cook MD, White BA, Holscher HD, Woods JA. Exercise Alters Gut Microbiota Composition and Function in Lean and Obese Humans. Med Sci Sports Exerc. 2018 Apr;50(4):747-757. doi: 10.1249/MSS.0000000000001495.

  PMID: 29166320 BACKGROUND

Biospecimen

Retention: SAMPLES WITH DNA

Frozen stool suspension

MeSH Terms

Conditions

Sedentary Behavior; Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Frédéric DERBRÉ, PhD

  Laboratory of Movement, Sport and health Sciences (M2S)

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: January 17, 2022
• First Posted: February 2, 2022
• Study Start: January 20, 2022
• Primary Completion: October 26, 2022
• Study Completion: November 23, 2022
• Last Updated: January 10, 2023

Record last verified: 2023-01

Data Sharing

• IPD Sharing: Will not share

Locations

FR (1)