Determining the Effect of Protein Quality on Post-exercise Anabolism in Active Youth

NCT05052827 | Unknown

• 1 other identifier: 36378
• Study Type: interventional
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

Leucine-enriched proteins (e.g., dairy) are more effective than plant-based sources for supporting post-exercise rates of muscle protein synthesis in adults. However, the impact of protein quality on protein synthesis and acute markers of growth in active children are unknown. Using a randomized crossover design, the investigators will compare the effects of ingesting 0.35g/kg fat free mass (FFM) of either milk protein concentrate (MPC), rice protein isolate (RPI), or soy protein isolate (SPI) following a bout of variable intensity exercise with whole-body protein kinetics (i.e., oxidation, synthesis, breakdown, and net balance) determined by oral \[13C\]phenylalanine tracer methodology.

Conditions

Dietary Proteins

Keywords

protein quality; growth; indicator amino acid oxidation; children; adolescent

Outcome Measures

Primary Outcomes (1)

• F13CO2

  Rate of 13CO2 excretion

  The rate of 13CO2 excretion based upon baseline (t=30 minutes) and isotopic steady state (t=360-420 minutes) 13CO2 enrichments in the breath and resting VCO2

Secondary Outcomes (1)

• Whole-Body Phenylalanine Kinetics

  Whole-Body Net Protein Balance will be calculated based upon baseline (t=30 minutes) and isotopic steady state (t=360-420 minutes) 13CO2 enrichments in the breath and 1-[13C]phenylalanine enrichments in the urine

Study Arms (3)

Children

ACTIVE COMPARATOR

Participants underwent 3 metabolic trials in a randomized crossover fashion, where they were provided with an isonitrogenous quantities of either milk protein concentrate, soy protein isolate, and rice protein isolate following a standardized bout of variable intensity exercise.

Dietary Supplement: Milk Protein Concentrate; Dietary Supplement: Rice Protein Isolate; Dietary Supplement: Soy Protein Isolate

Adolescent Females

ACTIVE COMPARATOR

Participants underwent 3 metabolic trials in a randomized crossover fashion, where they were provided with an isonitrogenous quantities of either milk protein concentrate, soy protein isolate, and rice protein isolate following a standardized bout of variable intensity exercise.

Dietary Supplement: Milk Protein Concentrate; Dietary Supplement: Rice Protein Isolate; Dietary Supplement: Soy Protein Isolate

Adolescent Males

ACTIVE COMPARATOR

Participants underwent 3 metabolic trials in a randomized crossover fashion, where they were provided with an isonitrogenous quantities of either milk protein concentrate, soy protein isolate, and rice protein isolate following a standardized bout of variable intensity exercise.

Dietary Supplement: Milk Protein Concentrate; Dietary Supplement: Rice Protein Isolate; Dietary Supplement: Soy Protein Isolate

Interventions

Milk Protein Concentrate DIETARY_SUPPLEMENT

Protein provided at 0.35 g/kg fat free-mass. Macronutrient and Amino Acid Content provided below. Per 100 g protein powder: Calories (Cal): 363 Carbs (g): 5.60 Protein (g): 82.60 Fat (g): 1.12 Total BCAA (g): 15.67 Total EAA (g): 34.66 Total AA (g): 83.27

Also known as: MPC, Idaho Milk Products (Grade A)

Adolescent Females; Adolescent Males; Children

Rice Protein Isolate DIETARY_SUPPLEMENT

Protein provided at 0.35 g/kg fat free-mass. Macronutrient and Amino Acid Content provided below. Per 100 g protein powder: Calories (Cal): 394 Carbs (g): 6.50 Protein (g): 86.70 Fat (g): 2.39 Total BCAA (g): 14.98 Total EAA (g): 30.62 Total AA (g): 86.11

Also known as: RPI, Axiom Foods - Organic Oryzatein Silk 90

Adolescent Females; Adolescent Males; Children

Soy Protein Isolate DIETARY_SUPPLEMENT

Protein provided at 0.35 g/kg fat free-mass. Macronutrient and Amino Acid Content provided below. Per 100 g protein powder: Calories (Cal): 377 Carbs (g): 2.12 Protein (g): 88.8 Fat (g): 0.60 Total BCAA (g): 13.88 Total EAA (g): 31.74 Total AA (g): 89.39

Also known as: MyProtein - Soy Protein Isolate

Adolescent Females; Adolescent Males; Children

Eligibility Criteria

• Age: 8 Years - 16 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17)

You may qualify if:

• Healthy will be defined as screened by the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone)
• Regularly active, as defined by being in the top 20% of age specific scores on the iPAQ (the International Physical Activity Questionnaire)
• Between the ages of 8 to 16 years
• An age and sex-specific minimum of 75th percentile Beep Test level

You may not qualify if:

• Inability to meet health and physical activity guidelines according to the PAR-Q+ and iPAQ
• Inability to adhere to any of the protocol guidelines
• Biological age outside of -0.5 to +1.5 years from PHV for adolescents, and \> -1 years from PHV for children

Sponsors & Collaborators

• University of Toronto: lead
• Dairy Management Inc.: collaborator

Study Sites (1)

Goldring Centre for High Performance Sport

Toronto, Ontario, M5S 2C9, Canada

Location

MeSH Terms

Interventions

Soybean Proteins

Intervention Hierarchy (Ancestors)

Plant Proteins; Proteins; Amino Acids, Peptides, and Proteins; Plant Proteins, Dietary; Dietary Proteins; Food; Diet, Food, and Nutrition; Physiological Phenomena; Soy Foods; Vegetable Products; Vegetables; Food and Beverages

Study Officials

• Daniel Moore, PhD

  University of Toronto

  PRINCIPAL INVESTIGATOR

• Hugo JW Fung, MSc

  University of Toronto

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Masking Details: As the study was a single-blind crossover design, the randomization of the protein type will be blinded to the participants only. The allocation concealment in relation to the liquid meal will be done by providing the same relative protein content (0.35 g/kg FFM) within the same volume of fluid, (8 ml/kg) to match the exercise-induced fluid loses, in opaque bottles. Additional carbohydrate (0.4g/kg) and artificial sweetener will be added to improve the organoleptic properties of the beverages.
• Purpose: OTHER
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Model Details: Using a single-blind randomized counterbalanced crossover design, participants will complete three metabolic trials providing 0.35 g/kg FFM of either milk protein concentrate (MPC), rice protein isolate (RPI), or soy protein isolate (SPI) following a bout of variable intensity exercise.

Study Record Dates

• First Submitted: September 13, 2021
• First Posted: September 22, 2021
• Study Start: September 1, 2021
• Primary Completion: December 1, 2023
• Study Completion: March 1, 2024
• Last Updated: November 13, 2023

Record last verified: 2023-11

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)