NCT04943380

Brief Summary

Prospective observational study to validate the performance characteristics and clinical utility of Cxbladder tests in a Veterans Affairs cohort.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
682

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Nov 2019

Longer than P75 for all trials

Geographic Reach
1 country

10 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 8, 2019

Completed
4 months until next milestone

First Submitted

Initial submission to the registry

March 2, 2020

Completed
1.3 years until next milestone

First Posted

Study publicly available on registry

June 29, 2021

Completed
2.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 18, 2024

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

January 18, 2024

Completed
Last Updated

April 24, 2026

Status Verified

April 1, 2026

Enrollment Period

4.2 years

First QC Date

March 2, 2020

Last Update Submit

April 21, 2026

Conditions

Outcome Measures

Primary Outcomes (2)

  • Proportion of participants with bladder cancer who are correctly identified as having cancer (true positives) and no cancer (true negatives) by the Cxbladder test.

    The Cxbladder test results will be compared to that of cystoscopy, which is the gold standard method for diagnosing urothelial cancer; the true positive and true negative rates will be measured, along with the false positive and false negative rates of the test. The results will be reported as sensitivity and specificity of the Cxbladder test for detecting urothelial cancer in patients with hematuria

    From enrolment to when a UC diagnosis is made or patient is considered UC negative (maximum period 6-months)

  • Probability that patients identified as having cancer and no cancer by the Cxbladder test truly have cancer (positive predictive value; PPV), and truly have no cancer (negative predictive value; NPV) respectively.

    The Cxbladder test results will be compared to that of cystoscopy, which is the gold standard method for diagnosing urothelial cancer; the true positive and true negative rates will be measured, along with the false positive and false negative rates of the test. The results will be reported as sensitivity and specificity of the Cxbladder test for detecting urothelial cancer in patients with recurrent disease.

    From enrolment to when a UC diagnosis is made or patient is considered UC negative (maximum period 6-months).

Secondary Outcomes (5)

  • To identify whether patients received a UC diagnosis 6- and 24-months post urine sample collection

    The outcome measure will be assessed by 6- and up to 24-months after trial completion.

  • Validation of performance characteristics of Cxbladder tests through sub-group analyses

    From enrolment to when a UC diagnosis is made or patient is considered UC negative (maximum period 6-months).

  • Comparison of the Cxbladder test's sensitivity, specificity, PPV and NPV with that of Cytology.

    From enrolment to when a UC diagnosis is made or patient is considered UC negative (maximum period 6-months).

  • To compare the theoretical clinical outcome of patients tested using Cxbladder signatures before investigative cystoscopy with evaluation by cystoscopy alone to establish the true proportion requiring cystoscopy.

    From enrolment to when a UC diagnosis is made or patient is considered UC negative (maximum period 6-months).

  • Performance characteristics of Cxbladder Triage and Cxbladder Detect compared with results generated at other clinical sites

    The outcome measure will be assessed from enrolment to when a UC diagnosis is made or patient is considered UC negative for this trial (maximum period 24-months) and completed following completion of other similar trials.

Study Arms (1)

patients with hematuria undergoing investigation for UC

Patients will be recruited from those presenting with hematuria and undergoing investigative cystoscopy for the determination of possible urothelial carcinoma. This includes patients referred via imaging or from other departments for assessment of hematuria. Microscopic hematuria is defined as ≥ 3 red blood cells per high-powered microscopy field for a properly collected urine sample.

Diagnostic Test: Cxbladder

Interventions

CxbladderDIAGNOSTIC_TEST

The Cxbladder Detect+ test is a molecular diagnostic test. The test consists of six DNA biomarkers and five RNA biomarkers measured in a small volume of urine. is an enhanced version of the Cxbladder Detect test.

patients with hematuria undergoing investigation for UC

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

This study will be conducted with all hematuria patients who present to an outpatient clinic to undergo clinical assessment including cystoscopy and who fulfil study requirements. Consented eligible patients will attend their scheduled clinic visit and undergo all standard tests as clinically indicated.

You may qualify if:

  • Patient is undergoing investigation of recent confirmed hematuria (by either flexible or rigid cystoscopy/TURBT), including hematuria patients referred due to suspicious/positive imaging, in order to determine the presence of urothelial carcinoma.
  • Able to provide a voided urine sample of the required minimum volume
  • Able to give written consent
  • Able and willing to comply with study requirements
  • Aged 18 years or older

You may not qualify if:

  • Prior history of bladder malignancy, prostate or renal cell carcinoma
  • Prior genitourinary manipulation (flexible or rigid cystoscopy / catheterisation, urethral dilation) in the 14 days before urine collection
  • Previous alkylating based chemotherapy
  • Known Pregnancy.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (10)

Veterans Medical Research Foundation

San Diego, California, 92161, United States

Location

Bay Pines Veterans Affairs

Bay Pines, Florida, 33744-8200, United States

Location

South Florida Veterans Affairs

Miami, Florida, 33125, United States

Location

James A Haley Veterans' Hospital

Tampa, Florida, 33612-4745, United States

Location

Minneapolis VA Health Care System

Minneapolis, Minnesota, 55417, United States

Location

NWIHCS-Omaha VA Medical Center

Omaha, Nebraska, 68105, United States

Location

James J. Peters VAMC

New York, New York, 10468-3904, United States

Location

Oklahoma City VA Hospital

Oklahoma City, Oklahoma, 73104, United States

Location

Medical University of South Carolina

Charleston, South Carolina, 29401, United States

Location

White River Junction VAMC

White River Junction, Vermont, 05009, United States

Location

Biospecimen

Retention: SAMPLES WITH DNA

A minimum of 30 mL of urine will be required for the sampling system to function. Two 5 mL aliquots of the collected urine will be transferred to a vacutainer containing a preservative reagent, and couriered to Pacific Edge Diagnostic NZ Laboratories.

MeSH Terms

Conditions

HematuriaCarcinoma, Transitional Cell

Condition Hierarchy (Ancestors)

Urination DisordersUrologic DiseasesFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesMale Urogenital DiseasesHemorrhagePathologic ProcessesPathological Conditions, Signs and SymptomsCarcinomaNeoplasms, Glandular and EpithelialNeoplasms by Histologic TypeNeoplasms

Study Officials

  • Tony Lough, PhD

    Pacific Edge Pty Ltd

    STUDY CHAIR

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

March 2, 2020

First Posted

June 29, 2021

Study Start

November 8, 2019

Primary Completion

January 18, 2024

Study Completion

January 18, 2024

Last Updated

April 24, 2026

Record last verified: 2026-04

Data Sharing

IPD Sharing
Will not share

Locations