The Influence of Exercise on Tissue Beta-alanine Uptake and Carnosine Synthesis Rates

NCT04936737 | Unknown

• 1 other identifier: B-alanine and HIIT
• Study Type: interventional
• Target: 14
• Locations: 1 country
• Sites: 1

Brief Summary

B-alanine supplementation is highly efficient in increasing intramuscular carnosine content, leading to improved physical performance, especially in high-intensity exercises (HIIE). It seems that exercise per se can modulate carnosine content; however, it remains uncertain whether physical training or training status can influence B-alanine supplementation responses. Thus, this work aims to assess whether HIIE can increase B-alanine uptake by peripheral tissues and, more specifically, skeletal muscle and increase intramuscular carnosine synthesis. The volunteers will be evaluated in two conditions: intake of B-alanine + exercise (B-EX) and B-alanine intake only (B-Ala). This process will be divided into two blocks of six days (Thursday to Thursday - without the weekend) with a 4-6 weeks washout. In the B-EX block, a 20-minute HIIE session will be held. In the B-Ala block, the same procedures will be adopted for the B-EX block, with the replacement of the HIIE for 20 minutes of rest sitting on the cycle ergometer. We will evaluate the determination of muscle B-alanine, plasma, and urine, the gene expression of carnosine-related enzymes and transporters, the enzymes Carnosine Synthase 1 (CARNS1), carnosine dipeptidase 2 (CN2), taurine transporter (TauT), PAT1, and phosphorylated Na + / K + / ATPase. The hypotheses are: 1) acute physical exercise increases the uptake of B-alanine by the skeletal muscle; 2) this effect is mediated by the increased activity of the Na + / K + / ATPase pump; 3) this effect, when repeated over five training sessions, results in observable increases in β-alanine → carnosine conversion in skeletal muscle.

Conditions

Exercise Training; Carnosine; Dietary Supplements

Outcome Measures

Primary Outcomes (2)

• Change from Baseline Muscle Carnosine after B-alanina supplementation

  HPLC-ESI+-MS/MS

  This evaluation will be carried out with the muscle samples collected on the first and the sixth (last) day of the protocol.

• Change from Baseline Plasma Beta-alanine after B-alanina supplementation

  HPLC-ESI+-MS/MS

  This evaluation will be carried out with the blood samples collected on the first and the sixth (last) day of the protocol

Study Arms (2)

Beta-alanine supplementation and high intensity exercise

EXPERIMENTAL

Individuals will participate in a high-intensity exercise and will be supplemented with beta-alanine. This protocol will be conducted for six non-consecutive days.

Dietary Supplement: Beta-alanine supplementation; Other: High intensity interval exercise

Beta-alanine supplementation only

EXPERIMENTAL

In this protocol, the individuals will be supplemented with beta-alanine without the exercise protocol for six non-consecutive days.

Dietary Supplement: Beta-alanine supplementation

Interventions

Beta-alanine supplementation DIETARY_SUPPLEMENT

The supplementation will be administrated in the dosage of 20mg.kg-1. On the first and the sixth (last one), one dose of beta-alanine will be taken. On the other four days of supplementation, the volunteers will take 2 doses (20mg.kg-1) of Beta-alanine.

Beta-alanine supplementation and high intensity exercise; Beta-alanine supplementation only

High intensity interval exercise OTHER

The HIIE sessions will consist of 10 series of 1 min of effort, divided into two blocks of 5 series each. There will be an interval of 5 min of passive recovery; within each block, the interval between sets will be 1 min of active recovery. The exercise will be conducted in a mechanical brake cycle ergometer, with load applied by placing washers on a load plate (Biotec 2100, Cefise, Brazil).

Beta-alanine supplementation and high intensity exercise

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• omnivorous diet for\> 6 months
• practice physical activities for\> 2 h · week-1
• stable body mass (variation \<2 kg in the last 30 days)

You may not qualify if:

• use of food supplements in the last six months containing β-alanine
• diagnosis of chronic diseases or any other condition that prevents them from performing the proposed procedures
• smoking
• current or past use of anabolic steroids or other ergogenic drugs
• Use of medications that may affect the assessments in this study, including insulin, GH, and thyroid hormone

Sponsors & Collaborators

• University of Sao Paulo: lead

Study Sites (1)

University of Sao Paulo

São Paulo, 05508-030, Brazil

RECRUITING

MeSH Terms

Interventions

High-Intensity Interval Training

Intervention Hierarchy (Ancestors)

Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Gabriella B Möller, Msc

  University of Sao Paulo

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Guilherme G Artioli, Phd

CONTACT

+551126481337; artioli@usp.br

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Model Details: The order of treatments will be random

Study Record Dates

• First Submitted: April 16, 2021
• First Posted: June 23, 2021
• Study Start: May 6, 2021
• Primary Completion: December 23, 2022
• Study Completion: December 31, 2023
• Last Updated: June 23, 2021

Record last verified: 2021-06

Locations

BR (1)