NCT04923555

Brief Summary

By 2050, the expanding world population will consume two-thirds more animal protein than it consumes today. The increase in chronic diseases associated with the generalization of these consumption patterns tend to understand the place of meat in our diets. All these elements participate to the reduction of animal proteins in favor of vegetable proteins in our food. The elderly are particularly affected by malnutrition, the prevalence of protein-energy malnutrition increasing with age and promoting the onset of morbidities. Without care, it leads to the worsening of physiological phenomena linked to aging such as loss of muscle functionality (sarcopenia) or reduction in bone density (osteoporosis) and increases the risk of falls - the main cause of dependence. However, in France, protein consumption declines significantly with age, even though requirements appear to be greater for the elderly. It is therefore a major challenge for our societies to ensure that the aging of the population and the increase in life expectancy are not synonymous with a reduction in the physical and mental capacities of individuals. Thus, it is essential to ensure that the recommendations for reducing the intake of animal proteins in favor of vegetable proteins can be applied without risk to aging populations, in particular on the human body cardiovascular risk of these populations.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
33

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Nov 2021

Shorter than P25 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

June 1, 2021

Completed
10 days until next milestone

First Posted

Study publicly available on registry

June 11, 2021

Completed
5 months until next milestone

Study Start

First participant enrolled

November 9, 2021

Completed
7 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 7, 2022

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

June 7, 2022

Completed
Last Updated

June 13, 2022

Status Verified

June 1, 2022

Enrollment Period

7 months

First QC Date

June 1, 2021

Last Update Submit

June 10, 2022

Conditions

Keywords

Metabolic syndromeEndothelial functionVegetable proteinsArginineCysteineLeucineNutrition assessmentNutrition physiological phenomena

Outcome Measures

Primary Outcomes (9)

  • Brachial artery Flow Mediated Dilation (FMD)

    The endothelial function will be assessed using the non-invasive ultrasound technique of flow mediated dilatation of the brachial artery. FMD measure is the percentage of dilation of brachial artery in response to a reactive hyperaemia induced by the release of a transient occlusion of the brachial artery realized.

    Day 0 (V1) at T-30min

  • Brachial artery Flow Mediated Dilation (FMD)

    The endothelial function will be assessed using the non-invasive ultrasound technique of flow mediated dilatation of the brachial artery. FMD measure is the percentage of dilation of brachial artery in response to a reactive hyperaemia induced by the release of a transient occlusion of the brachial artery realized between T-30min to T300min.

    Day 0 (V1) at T180min

  • Brachial artery Flow Mediated Dilation (FMD)

    The endothelial function will be assessed using the non-invasive ultrasound technique of flow mediated dilatation of the brachial artery. FMD measure is the percentage of dilation of brachial artery in response to a reactive hyperaemia induced by the release of a transient occlusion of the brachial artery realized between T-30min to T300min.

    Day 0 (V1) at T300min

  • Brachial artery Flow Mediated Dilation (FMD)

    The endothelial function will be assessed using the non-invasive ultrasound technique of flow mediated dilatation of the brachial artery. FMD measure is the percentage of dilation of brachial artery in response to a reactive hyperaemia induced by the release of a transient occlusion of the brachial artery realized between T-30min to T300min.

    Day 28 (V2) at T-30min

  • Brachial artery Flow Mediated Dilation (FMD)

    The endothelial function will be assessed using the non-invasive ultrasound technique of flow mediated dilatation of the brachial artery. FMD measure is the percentage of dilation of brachial artery in response to a reactive hyperaemia induced by the release of a transient occlusion of the brachial artery realized between T-30min to T300min.

    Day 28 (V2) at T180min

  • Brachial artery Flow Mediated Dilation (FMD)

    The endothelial function will be assessed using the non-invasive ultrasound technique of flow mediated dilatation of the brachial artery. FMD measure is the percentage of dilation of brachial artery in response to a reactive hyperaemia induced by the release of a transient occlusion of the brachial artery realized between T-30min to T300min.

    Day 28 (V2) at T300min

  • Brachial artery Flow Mediated Dilation (FMD)

    The endothelial function will be assessed using the non-invasive ultrasound technique of flow mediated dilatation of the brachial artery. FMD measure is the percentage of dilation of brachial artery in response to a reactive hyperaemia induced by the release of a transient occlusion of the brachial artery realized between T-30min to T300min.

    Day 56 (V3) at T-30min

  • Brachial artery Flow Mediated Dilation (FMD)

    The endothelial function will be assessed using the non-invasive ultrasound technique of flow mediated dilatation of the brachial artery. FMD measure is the percentage of dilation of brachial artery in response to a reactive hyperaemia induced by the release of a transient occlusion of the brachial artery realized between T-30min to T300min.

    Day 56 (V3) at T180min

  • Brachial artery Flow Mediated Dilation (FMD)

    The endothelial function will be assessed using the non-invasive ultrasound technique of flow mediated dilatation of the brachial artery. FMD measure is the percentage of dilation of brachial artery in response to a reactive hyperaemia induced by the release of a transient occlusion of the brachial artery realized between T-30min to T300min.

    Day 56 (V3) at T300min

Secondary Outcomes (79)

  • Rest flow by Flowmetry Laser Doppler (FLD)

    Day 0 (V1), Day 28 (V2), Day 56 (V3)

  • Occlusion area by FLD

    Day 0 (V1), Day 28 (V2), Day 56 (V3)

  • Hyperaemia area by FLD

    Day 0 (V1), Day 28 (V2), Day 56 (V3)

  • Hyperaemia area / occlusion area ratio by FLD

    Day 0 (V1), Day 28 (V2), Day 56 (V3)

  • Maximal flow by FLD

    Day 0 (V1), Day 28 (V2), Day 56 (V3)

  • +74 more secondary outcomes

Study Arms (3)

Group/Cohort1

EXPERIMENTAL

33 subjects aged 65 years old with predisposition to cardiometabolic syndrome will consume 400ml of yogurt rich in cysteine, leucine and arginine (VP, vegetable proteins) only once during the visit

Behavioral: Vegetable proteins (VP) rich in leucine, cystein, arginineBehavioral: Animal proteins (AP)Behavioral: No protein (T)

Group/Cohort2

EXPERIMENTAL

33 subjects aged 65 years old with predisposition to cardiometabolic syndrome will consume 400ml of yogurt rich in animal proteins (AP) only once during the visit

Behavioral: Vegetable proteins (VP) rich in leucine, cystein, arginineBehavioral: Animal proteins (AP)Behavioral: No protein (T)

Group/Cohort3

PLACEBO COMPARATOR

33 subjects aged 65 years old with predisposition to cardiometabolic syndrome will consume 400ml of yogurt without any proteins (T) only once during the visit

Behavioral: Vegetable proteins (VP) rich in leucine, cystein, arginineBehavioral: Animal proteins (AP)Behavioral: No protein (T)

Interventions

33 volunteers will consume 400 ml of yogurt with vegetable proteins (VP) rich in leucine, cystein and arginine only once during the visit. At the beginning and the end of the intervention, exploration will be conducted at fasted state and at post-prandial state after the administration of vegetable proteins.

Group/Cohort1Group/Cohort2Group/Cohort3

33 volunteers will consume 400 ml of yogurt with animal proteins (AP) rich in leucine, cystein and arginine only once during the visit. At the beginning and the end of the intervention, exploration will be conducted at fasted state and at post-prandial state after the administration of animal proteins.

Group/Cohort1Group/Cohort2Group/Cohort3
No protein (T)BEHAVIORAL

33 volunteers will consume 400 ml of yogurt without any protein (T) rich in leucine, cystein and arginine only once during the visit. At the beginning and the end of the intervention, exploration will be conducted at fasted state and at post-prandial state after the administration of animal proteins.

Group/Cohort1Group/Cohort2Group/Cohort3

Eligibility Criteria

Age65 Years+
Sexall
Healthy VolunteersYes
Age GroupsOlder Adult (65+)

You may qualify if:

  • Man or woman
  • years old and older (inclusive)
  • At least 2 of the following 4 cardiometabolic factors:
  • Waist circumference ≥88 cm for women, and ≥94 cm for men
  • Fasting triglyceridemia \>1,5 g/L OR HDL level \< 40 mg/dl for men, and \< 50 mg/dl for women
  • Fasting blood glucose ≥ 100 mg/dl
  • Systolic blood pressure \>130mmHg ou diastolic \> 85 mm Hg
  • Accept not to change his lifestyle throughout the study
  • Accept to consume the same meal the day before exploration days, making sure to exclude non-recommended foods and agreeing to detail its content in a food diary
  • Ability to give informed consent to participate in research
  • Affiliation to Social Security

You may not qualify if:

  • Acute pathology (unstable or terminal pathology)
  • Renal failure (clearance \<40 mL / min)
  • Asthma or chronic respiratory disease
  • Systolic or diastolic blood pressure in the judgement of the investigator
  • Diabetic (treated or not)
  • Treated with chemotherapy
  • Gastrointestinal, thyroid, cardiac or vascular illness in the judgement of the investigator
  • Biological examination no compatible with the study in the judgement of the investigator
  • Medical and/or surgical history no compatible with the study in the judgement of the investigator (previous cardiovascular events)
  • AgHbS, AcHbc, HCV and HIV positive serology
  • Concomitant treatment no compatible with the study in the judgement of the investigator
  • Diet or change in body mass \> 2 kg in the 30 days before the study
  • Allergies to any of the components of the test meals
  • Alcohol consumption\> 2 glasses / day
  • Current smokers (\> 6 cigarettes per week)
  • +2 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

CHU clermont-ferrand

Clermont-Ferrand, France

Location

Related Publications (1)

  • Dimina LJ, Leray V, Voute M, David J, Blavignac C, Farges MC, Rossary A, Tsikas D, Remond D, Pickering G, Mariotti F. Dietary Protein in a Challenge Meal Does Not Alleviate Postprandial Impairments in Vascular Endothelial Function in Healthy Older Adults with Cardiometabolic Risk: A Randomized Crossover-Controlled Trial. J Nutr. 2024 Dec;154(12):3664-3680. doi: 10.1016/j.tjnut.2024.10.018. Epub 2024 Oct 16.

MeSH Terms

Conditions

Metabolic Syndrome

Interventions

Plant Proteins, DietaryLeucineArginineAnimal Proteins, Dietary

Condition Hierarchy (Ancestors)

Insulin ResistanceHyperinsulinismGlucose Metabolism DisordersMetabolic DiseasesNutritional and Metabolic Diseases

Intervention Hierarchy (Ancestors)

Dietary ProteinsProteinsAmino Acids, Peptides, and ProteinsPlant ProteinsFoodDiet, Food, and NutritionPhysiological PhenomenaFood and BeveragesAmino Acids, Branched-ChainAmino AcidsAmino Acids, EssentialAmino Acids, BasicAmino Acids, Diamino

Study Officials

  • Gisèle PICKERING

    University Hospital, Clermont-Ferrand

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Masking Details
Product kit (yogurt) will be labelled and packaged according to the pre-established randomization plane by the pharmacy department of University Hospital of Clermont-Ferrand, France. Kits will be distributed in a blind fashion for each cross over period on the basis of the randomization schedule.
Purpose
BASIC SCIENCE
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 1, 2021

First Posted

June 11, 2021

Study Start

November 9, 2021

Primary Completion

June 7, 2022

Study Completion

June 7, 2022

Last Updated

June 13, 2022

Record last verified: 2022-06

Locations