Gene Expression Changes of Brain Tissue in Human Temporal Lobe Epilepsy With Hippocampal Sclerosis

NCT04917939 | Active Not Recruiting

• 1 other identifier: 2021-0291
• Study Type: observational
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

Epilepsy is a common neurological disease, manifested in the sudden abnormal discharge of neurons leading to short-term brain dysfunction, has become the neurology after headache the second most common disease. In China, the prevalence of epilepsy is about 4.7-8.5 per 1,000, and more than 400,000 new cases of epilepsy are developed each year. Of these, 30% of patients were treated with ineffective medication, developing into a drug-incurable epilepsy that required surgery and other treatments. The most common type of epilepsy is temporal lobe epilepsy, while the common complication in temporal lobe epilepsy is hippocampal sclerosis, which often requires surgical removal. The incidence of inner temporal lobe epilepsy associated with hippocampal sclerosis is increasing, but its exact cause and specific pathogenesis are still unclear, so clarifying its pathogenesis will contribute to the understanding of temporal lobe epilepsy and the improvement of surgical procedures. This study is intended to get single-cell transcriptome as well as spatial transcriptome data of temporal lobe and hippocampus samples. By studying gene expression change associated with epilepsy and hippocampus sclerosis, we intended to find possible prognostic-related molecules and to deepen understanding of pathological changes in epilepsy at the molecular level.

Conditions

Epilepsy; Hippocampal Sclerosis

Keywords

Epilepsy; Hippocampal Sclerosis

Outcome Measures

Primary Outcomes (1)

• RNA sequencing data

  Single-cell sequencing and spatial transcription sequencing data of samples.

  up to 24 weeks

Study Arms (2)

epilepsy with HS

Other: RNA sequencing

epilepsy without HS

Other: RNA sequencing

Interventions

RNA sequencing OTHER

Samples in both groups will be sequenced by single-cell sequencing and spatial transcription sequencing.

epilepsy with HS; epilepsy without HS

Eligibility Criteria

• Sex: all
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Epilepsy patients with or without hippocampal sclerosis.

You may qualify if:

• Patients confirmed in our hospital as having temporal lobe epilepsy and intending surgery to remove temporal lobe and hippocampal tissue;
• Volunteer to participate in this clinical trial, comply with the requirements of this clinical trial and sign an informed consent form.

You may not qualify if:

• Chronic diseases of the central nervous system other than temporal lobe epilepsy;
• Other patients that are not suitable for this clinical trial determined by the researchers;

Sponsors & Collaborators

• Second Affiliated Hospital, School of Medicine, Zhejiang University: lead

Study Sites (1)

2ndAffiliated Hospital, School of Medicine, Zhejiang University, China

Hangzhou, Zhejiang, China

Location

MeSH Terms

Conditions

Epilepsy; Hippocampal Sclerosis

Interventions

Sequence Analysis, RNA

Condition Hierarchy (Ancestors)

Brain Diseases; Central Nervous System Diseases; Nervous System Diseases; Malformations of Cortical Development, Group I; Malformations of Cortical Development; Nervous System Malformations; Congenital Abnormalities; Congenital, Hereditary, and Neonatal Diseases and Abnormalities

Intervention Hierarchy (Ancestors)

Sequence Analysis; Genetic Techniques; Investigative Techniques

Study Officials

• 2nd Affiliated Hospital

  2nd Affiliated Hospital, School of Medicine, Zhejiang University, China

  STUDY CHAIR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Chief Physician

Study Record Dates

• First Submitted: June 7, 2021
• First Posted: June 8, 2021
• Study Start: April 19, 2021
• Primary Completion (Estimated): December 31, 2026
• Study Completion (Estimated): December 31, 2026
• Last Updated: April 27, 2026

Record last verified: 2026-03

Locations

CN (1)