A Study to Evaluate the Safety and Anti-tumor Activity of SNK01 (NK Cells) Administered in Combination With Chemotherapy or Chemotherapy / Cetuximab in Local Advanced or Metastatic Non-small Cell Lung Cancer Patients Who Failed Tyrosine Kinase Inhibitor Treatment

NCT04872634 | Unknown Phase 1

• 1 other identifier: SNK01_TRN12a
• Study Type: interventional
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

This clinical trial is a Single-Center, Open, Phase I/IIa Clinical Trial conducted to evaluate the safety and anti-tumor activity of SNK01 and GC +/- Cetuximab administered in combination to Locally advanced or Metastatic Non-small Cell Lung Cancer Patients who have failed prior Tyrosine Kinase Inhibitor (TKI) therapy at least once. After the start of the clinical trial, the first 3 subjects complete the enrollment in Cohort 1 in serial order and then 3 subjects are enrolled in Cohort 2 in serial order. After this, Cohorts 1, 3 and Cohorts 2, 4 are independently processed and subjects are enrolled in serial order when new cohorts start and/or replacement subjects are required. For the subjects who are additionally enrolled after the DLT evaluation and the MTD is determined in each dose cohort, no DLT evaluation is conducted. The subjects allotted to each cohort are administered with the SNK01 manufactured from peripheral blood mononuclear cells total 8 times over a period of about 10 weeks. Combined administration of SNK01 starts from the Cycle 2 (Week 4) of Cytotoxic Chemotherapy and SNK01 is administered at an interval of 1 week starting from the day after the administration of Cytotoxic Chemotherapy and/or Cetuximab (Visit 5-1, D23). When no disease progression is confirmed at EOT (End of Treatment), disease progression is checked until the clinical trial is over. The adverse events which have occurred during the study period are monitored until the date when the investigator judges that no monitoring is required as the symptom has disappeared or there is no further change in the symptom or the 30th day (±3 d) from the latest date of the administration among Gemcitabine, Carboplatin and Cetuximab after the EOT, whichever comes first. For all the subjects enrolled in the present clinical trial, safety is checked in accordance with CTCAE V5.0 and effectiveness is checked in accordance with RECIST V1.1 through the vital signs, laboratory test, adverse events, etc. during the study period.

Conditions

Non-small Cell Lung Cancer

Outcome Measures

Primary Outcomes (2)

• Maximum Tolerated Dose of the dose of SNK01 + GC + (or Cetuximab)

  Dose-Limiting Toxicity assessment

  28 days

• Adverse event

  Adverse event (AE)

  Up to 12 months

Secondary Outcomes (7)

• Progression-free survival

  every 12 weeks, up to the time of death or tumor progression, maximum 24 months

• Disease Control Rate

  every 12 weeks, up to the time of death or tumor progression, maximum 24 months

• objective response rate

  every 12 weeks, up to the time of death or tumor progression, maximum 24 months

• Time to progression

  every 12 weeks, up to the time of death or tumor progression, maximum 24 months

• Duration of response

  every 12 weeks, up to the time of death or tumor progression, maximum 24 months

Study Arms (4)

SNK01 (4ⅹ10^9 cells) + GC

EXPERIMENTAL

Administration of SNK01 4ⅹ10\^9 cells/dose 8 times at an interval of 1 week + Administration of Cytotoxic Chemotherapy (GC) up to 4 cycles at an interval of 3 weeks

Biological: SNK01 (Super Natural Killer Cells 01); Drug: GC

SNK01 (4ⅹ10^9 cells) + GC + Cetuximab

EXPERIMENTAL

Administration of SNK01 4ⅹ10\^9 cells/dose 8 times at an interval of 1 week + Administration of Cytotoxic Chemotherapy (GC) up to 4 cycles at an interval of 3 weeks + Weekly administration of Cetuximab until the disease progresses or unacceptable toxicity develops

Biological: SNK01 (Super Natural Killer Cells 01); Drug: GC; Biological: Cetuximab

SNK01 (6ⅹ10^9 cells) + GC

EXPERIMENTAL

Administration of SNK01 6ⅹ10\^9 cells/dose 8 times at an interval of 1 week + Administration of Cytotoxic Chemotherapy (GC) up to 4 cycles at an interval of 3 weeks

Biological: SNK01 (Super Natural Killer Cells 01); Drug: GC

SNK01 (6ⅹ10^9 cells) + GC + Cetuximab

EXPERIMENTAL

Administration of SNK01 6ⅹ10\^9 cells/dose 8 times at an interval of 1 week + Administration of Cytotoxic Chemotherapy (GC) up to 4 cycles at an interval of 3 weeks + Weekly administration of Cetuximab until the disease progresses or unacceptable toxicity develops

Biological: SNK01 (Super Natural Killer Cells 01); Drug: GC; Biological: Cetuximab

Interventions

SNK01 (Super Natural Killer Cells 01) BIOLOGICAL

Investigational Product : SNK01 (Super Natural Killer Cells 01) Principal Component : Autologous Natural Killer Cell To induce anti-tumor immunity in Locally advanced or Metastatic Non-small Cell Lung Cancer Patients who have failed prior TKI therapy for EGFR, ALK, or ROS1 Gene Mutation and to strengthen the Antibody-Dependent Cellular Cytotoxicity (ADCC) of Cetuximab, 4x10\^9 or 6x10\^9 cells are infused through the veins of the subjects within 45 minutes (±15 minutes) per 100 mL total 8 times at intervals of one week.

SNK01 (4ⅹ10^9 cells) + GC; SNK01 (4ⅹ10^9 cells) + GC + Cetuximab; SNK01 (6ⅹ10^9 cells) + GC; SNK01 (6ⅹ10^9 cells) + GC + Cetuximab

GC DRUG

Gemcitabine; 1000mg/m2, Day 1, Day 8, 4-cycle administration, Mixed with normal saline 50ml and administered into a vein for 30 min. Carboplatin; AUC 5, Day 1, 4-cycle administration, Mixed with 5% Dextrose 200ml and administered into a vein for 1 hour.

Also known as: Cytotoxic Chemotherapy

SNK01 (4ⅹ10^9 cells) + GC; SNK01 (4ⅹ10^9 cells) + GC + Cetuximab; SNK01 (6ⅹ10^9 cells) + GC; SNK01 (6ⅹ10^9 cells) + GC + Cetuximab

Cetuximab BIOLOGICAL

First administration: 400mg/m2 Subsequent administration: 250mg/m2 (Premedication before administration\*) Weekly Administration continues until the disease progresses or unacceptable toxicity develops. 400mg/m2: Administered into a vein for 120 minutes 250mg/m2: Administered into a vein for 60 minutes

SNK01 (4ⅹ10^9 cells) + GC + Cetuximab; SNK01 (6ⅹ10^9 cells) + GC + Cetuximab

Eligibility Criteria

• Age: 20 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• histologically or cytologically diagnosed with Locally advanced or Metastatic Non-small Cell Lung Cancer (TNM 8th edition). However, recurrent Non-small Cell Lung Cancer is also allowed.
• one or more gene mutations among EGFR, ALK, and ROS1
• TKI treated for EGFR, ALK, or ROS1 gene mutation at least once or whose progressive disease is confirmed during or after completion of the treatment
• platinum based chemotherapy (excluding Gemcitabine), other chemotherapy or immunotherapy besides TKI treatment before. (Those who have undergone neoadjuvant chemotherapy or adjuvant chemotherapy are also allowed.)
• remaining toxicity resulting from previous anti-cancer therapy is grade 1 or lower in accordance with CTCAE V5.0. However, those who suffer from alopecia not lower than grade 2 or neuropathy of grade 2 related to previous platinum based chemotherapy are allowed to be enrolled.
• voluntarily decided to participate in the present clinical trial and put their signature on a subject's consent form before start of the trial
• years of age or older
• at least one lesion measurable by RECIST v1.1
• life expectancy is 3 months or longer
• Eastern Collaborative Oncology Group (ECOG) performance status is 0 or 1 when screened
• adequate blood and terminal organ functions
• For fertile women: Those who have agreed to maintain abstinence (refraining from sextual intercourses with men) or use a contraceptive measure of which the annual failure rate is lower than 1 % during the treatment period and for minimum 6 months (180 days) after the last administration of SNK01, GC, or Cetuximab. Women who have had their first menstrual period, not yet reached a post-menopausal state (amenorrhea for longer than 12 months in succession without cause other than menopause) and have not undergone surgical sterilization (ovariectomy or hysterectomy) and under the age of 55 with follicle stimulating hormone (FSH) levels of less than 40 mIU/mL are considered as fertile women. However, women who have already confirmed menopause are excluded. The examples of the contraceptive measures of which the failure rate is lower than 1 % include the following: Bilateral Tubal Ligatio, male sterilization, established and appropriate use of hormonal contraception that inhibits ovulation, hormone releasing IUD (IntraUterine Device) and copper IUD. The reliability of abstinence should be evaluated based on the clinical trial period and the daily life-style preferred by the patient. Periodic abstinence (example: calendar method, ovulation, and post-ovulation body temperature measurement method) and coitus interrupts are not acceptable contraceptive measures.
• For men: Those who have agreed to maintain abstinence (refraining from sextual intercourses with women) as defined below, use contractiptive measures, and abstain from sperm donation. Men with a fertile spouse should maintain abstinence or use of condoms and additional contraceptive measures during the treatment period and for minimum 4 months (120 days) after the last administration of SNK01, GC or Cetuximab so that the annual failure rate is lower than 1 %. Men should abstain from sperm donation during this period. The reliability of sexual abstinence should be evaluated based on the study period and the normal life-style preferred by the patient. Periodic abstinence (example: calendar method, ovulation, and post-ovulation body temperature measurement method) and coitus interrupts are not acceptable contraceptive measures.
• adapt themselves to the Protocol according to the judgment of the investigator

You may not qualify if:

• have taken a drug for another clinical trial or participated in another clinical trial within 30 days prior to the enrollment
• have taken Cetuximab to cure Non-Small Cell Lung Cancer
• a systemic or partial antineoplastic therapy is scheduled during the period of the present study
• previous treatments meet the following criteria:
• Those who have undergone systemic Cytotoxic Chemotherapy, administration of a biologic agent, or a surgery within 3 weeks prior to the enrollment date
• Those who have undergone Breast Radiotherapy of which the radiation exceeds 30 gray (Gy) for treatment of Non-small Cell Lung Cancer within 6 months prior to the enrollment date
• Those who have undergone Palliative Radiotherapy on the regions to which a lung cancer is metastasized (bone, brain, etc.) within 1 week before the enrollment date
• Those who have undergone therapy of EGFR TKIs (Gefitinib, Erlotinib, Afatinib, Osimertinib, etc.), ALK TKIs (Crizotinib, Ceretinib, Alectinib, Brigatinib, etc.) and/or ROS1 TKIs (Crizotinib, etc.) within 2 weeks of the enrollment date
• an anamnesis of a known allergic reaction or a serious allergic reaction to Gemcitabine, Carboplatin, or another platinum containing compound, Cetuximab or a monoclonal antibody
• uncured or active Central Nervous System (CNS) metastasis confirmed by screening and previous CT or MRI evaluation. However, the patients who have cured or symptomless CNS metastasis are allowed to be enrolled if they meet all the following criteria:
• if a lesion exists on the region other than the area of CNS metastasis that can be evaluated or measured by RECIST V1.1;
• if it is radiologically confirmed before the enrollment that the lesion is stabilized after the area of CNS metastasis has been treated;
• if the patients have no anamnesis of intracranial hemorrhage or myelorrhagia; and
• if the patients have clinically stable symptomless lesion for minimum 2 weeks after treatment of the CNS metastasis and stopping use of anticonvulsant or steroid.
• an active autoimmune disorder within 2 years prior to the enrollment date which requires systemic treatment

Sponsors & Collaborators

• NKMAX Co., Ltd.: lead

Study Sites (1)

Asan Medical Center

Seoul, Songpa-gu, 05505, South Korea

RECRUITING

Related Publications (1)

• Choi MG, Son GW, Choi MY, Jung JS, Rho JK, Ji W, Yoon BG, Jo JM, Kim YM, Ko DH, Lee JC, Choi CM. Safety and efficacy of SNK01 (autologous natural killer cells) in combination with cytotoxic chemotherapy and/or cetuximab after failure of prior tyrosine kinase inhibitor in non-small cell lung cancer: non-clinical mouse model and phase I/IIa clinical study. J Immunother Cancer. 2024 Mar 27;12(3):e008585. doi: 10.1136/jitc-2023-008585.

  PMID: 38538093 DERIVED

MeSH Terms

Conditions

Carcinoma, Non-Small-Cell Lung

Interventions

Cytotoxins; Cetuximab

Condition Hierarchy (Ancestors)

Carcinoma, Bronchogenic; Bronchial Neoplasms; Lung Neoplasms; Respiratory Tract Neoplasms; Thoracic Neoplasms; Neoplasms by Site; Neoplasms; Lung Diseases; Respiratory Tract Diseases

Intervention Hierarchy (Ancestors)

Noxae; Toxic Actions; Chemical Actions and Uses; Antibodies, Monoclonal, Humanized; Antibodies, Monoclonal; Antibodies; Immunoglobulins; Immunoproteins; Blood Proteins; Proteins; Amino Acids, Peptides, and Proteins; Serum Globulins; Globulins

Study Design

• Study Type: interventional
• Phase: phase 1
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: INDUSTRY
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: April 21, 2021
• First Posted: May 4, 2021
• Study Start: July 8, 2021
• Primary Completion: May 26, 2023
• Study Completion: May 26, 2023
• Last Updated: July 21, 2021

Record last verified: 2021-07

Data Sharing

• IPD Sharing: Will not share

Locations

KR (1)