NCT04785144

Brief Summary

This is a phase 1, open-label, randomized clinical trial in males and non-pregnant females, 18 years of age and older, who are in good health, have no known history of COVID-19 or SARS-CoV-2 infection, and meet all other eligibility criteria. This clinical trial is designed to assess the safety, reactogenicity and immunogenicity of mRNA-1273.351 manufactured by ModernaTX, Inc, given in vaccination schedules alone, sequentially, or coadministered with mRNA-1273. mRNA-1273.351 is a novel lipid nanoparticle (LNP)-encapsulated mRNA-based vaccine that encodes for a full-length, prefusion stabilized S protein of the SARS-CoV-2 B.1.351 variant. Enrollment will occur at approximately five domestic clinical research sites. This study includes two cohorts. Cohort 1 will provide rapid information about the immunogenicity of mRNA-1273.351 in a previously vaccinated group. This cohort can inform near term public health decisions if the variant virus becomes more widespread. Cohort 2 will evaluate different strategies for generation of cross protective immune responses in a naïve population. This cohort will take longer to provide information on the immunogenicity of mRNA-1273.351, but is important to inform future public health strategies. Cohort 1 will include approximately 60 subjects 18 years of age and older who received two vaccinations of mRNA-1273 at dosages of 50 mcg, 100 mcg, or 250 mcg in the Phase 1 clinical trial (DMID 20-0003). Subjects in Cohort 1 will receive a single intramuscular (IM) injection of the designated vaccine and will be followed through 12 months after vaccination. Follow-up visits will occur on Days 8, 15, and 29, as well as 3, 6, and 12 months after the vaccination. Cohort 2 will include approximately 150 participants 18 through 55 years of age who have not received a COVID-19 vaccine, have no known history of COVID-19 or SARS-CoV-2 infection, and do not have underlying conditions that are associated with an increased risk of severe illness from SARS-CoV-2 infection. Enrollment may close before the full 150 participants based on estimates on the timing of immunogenicity results and the need to inform public health decisions. They will be randomly assigned to one of 8 treatment arms and will receive 2 or 3 IM injections of the vaccine and followed through 12 months after the last vaccination. Follow-up visits will occur 7, 14, and 28 days after each vaccination, as well as 3, 6 and 12 months post the last vaccination. The primary objective is to evaluate the safety and reactogenicity of mRNA-1273 and mRNA-1273.351 vaccines, in naïve and previously vaccinated individuals.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
135

participants targeted

Target at P75+ for phase_1 covid19

Timeline
Completed

Started Mar 2021

Typical duration for phase_1 covid19

Geographic Reach
1 country

4 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

March 4, 2021

Completed
1 day until next milestone

First Posted

Study publicly available on registry

March 5, 2021

Completed
26 days until next milestone

Study Start

First participant enrolled

March 31, 2021

Completed
1.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 5, 2022

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

July 5, 2022

Completed
1.2 years until next milestone

Results Posted

Study results publicly available

September 13, 2023

Completed
Last Updated

March 18, 2024

Status Verified

April 6, 2021

Enrollment Period

1.3 years

First QC Date

March 4, 2021

Results QC Date

June 22, 2023

Last Update Submit

March 14, 2024

Conditions

Keywords

CoronavirusCOVID-19ImmunogenicitymRNA-1273mRNA-1273.351SARS-CoV-2SARS-CoV-2 B.1.351vaccine

Outcome Measures

Primary Outcomes (8)

  • Number of Participants With Any Medically-attended Adverse Events (MAAEs)

    Number of participants that experienced MAAEs during the course of the study.

    Day 1 to study completion, up to 1 year post last dose

  • Frequency of Any New-onset Chronic Medical Conditions (NOCMCs)

    Number of participants that experienced any NOCMCs during the course of the study.

    Day 1 to study completion, up to 1 year post last dose

  • Frequency of Any Protocol Specified Adverse Events of Special Interest (AESIs)

    Number of participants that experienced any AESIs during the course of the study. An adverse event of special interest (serious or non-serious) is one of scientific and medical concern specific to the sponsor's product or program, for which ongoing monitoring and rapid communication by the investigator to the sponsor is required. AESIs include anosmia, ageusia, subacute thyroiditis, acute pancreatitis, appendicitis, rhabdomyolysis, acute respiratory distress syndrome, coagulation disorders, acute cardiovascular injury, acute kidney injury, acute liver injury, dermatologic findings, multisystem inflammatory disorders, thrombocytopenia, acute aseptic arthritis, new onset of or worsening of neurologic disease, anaphylaxis, and other syndromes.

    Day 1 to study completion, up to 1 year post last dose

  • Frequency of Any Serious Adverse Events (SAEs)

    The number of participants that experience any SAEs from Day 1 to study completion. An AE or suspected adverse reaction is considered serious if, in the view of either the participating site PI or appropriate sub-investigator or the sponsor, it results in: death, a life-threatening AE, inpatient hospitalization or prolongation of existing hospitalization, a persistent or significant incapacity or substantial disruption of the ability to conduct normal life functions, or a congenital anomaly/birth defect.

    Day 1 to study completion, up to 1 year post last dose

  • Frequency of Solicited Reactogenicity Adverse Events (AEs)

    The number of participants that experienced at least one solicited (local and systemic) AE through 7 days post vaccination. Systemic events include: fatigue, headache, myalgia, arthralgia, nausea, chills and fever. Local events include: pain at injection site, erythema, and induration.

    Day 1 through Day 7 for Arms 1A-2H, Day 29 through Day 36 for Arms 2A-2H, Day 57-64 for Arms 2A-2B

  • Grade of Solicited Reactogenicity Adverse Events (AEs)

    Number of participants who experienced any solicited (local and systemic) AEs through 7 days post vaccination by grade. Systemic events include: fatigue, headache, myalgia, arthralgia, nausea, chills and fever. Local events include: pain at injection site, erythema, and induration.

    Day 1 through Day 7 for Arms 1A-2H, Day 29 through Day 36 for Arms 2A-2H, Day 57-64 for Arms 2A-2B

  • Frequency of Unsolicited Adverse Events (AEs) by Relationship to Study Product

    Number of events of any unsolicited AE through 28 days post vaccination by relationship to study product and severity, experienced by participants in the Safety Analysis population. Unsolicited AEs were events that were spontaneously reported by the subject, or revealed by observation, physical examination or other diagnostic procedures.

    Day 1 through Day 29 for Arms 1A-2H, Day 29 through Day 57 for Arms 2A-2H, Day 57 through Day 85 from Arms 2A-2B

  • Grade of Any Unsolicited Adverse Events (AEs)

    Number of any unsolicited AEs through 28 days post vaccination by severity.

    Day 1 through Day 29 for Arms 1A-2H, Day 29 through Day 57 for Arms 2A-2H, Day 57 through Day 85 from Arms 2A-2B

Secondary Outcomes (24)

  • Geometric Mean Titer (GMT) of Antibody Against WA-1 S2-P for Arms 1A and 1B

    Day 1 Pre-Booster Dose, Day 15, Day 29, Day 91, Day 181, Day 366

  • Geometric Mean Titer (GMT) of Antibody Against WA-1 S2-P for Arms 2A and 2B

    Day 1 Pre-Dose, Day 15, Day 29, Day 43, Day 57, Day 71, Day 85, Day 147, Day 237, Day 422

  • Geometric Mean Titer (GMT) of Antibody Against WA-1 S2-P for Arms 2C-H

    Day 1 Pre-Dose, Day 15, Day 29, Day 43, Day 57, Day 119, Day 209, Day 394

  • Geometric Mean Titer (GMT) of Antibody Against B.1.351 S2-P for Arms 1A and 1B

    Day 1 Pre-Booster Dose, Day 15, Day 29, Day 91, Day 181, Day 366

  • Geometric Mean Titer (GMT) of Antibody Against B.1.351 S2-P for Arms 2A and 2B

    Day 1 Pre-Dose, Day 15, Day 29, Day 43, Day 57, Day 71, Day 85, Day 147, Day 237, Day 422

  • +19 more secondary outcomes

Study Arms (10)

Arm 1A

EXPERIMENTAL

50 mcg of mRNA-1273.351 administered through 0.5 mL intramuscular injection in the deltoid muscle on Day 1 in participants who received two vaccinations of mRNA-1273 in DMID Protocol 20-0003 (NCT04283461). N=30.

Biological: mRNA-1273.351

Arm 1B

EXPERIMENTAL

25 mcg of mRNA-1273 and 25 mcg of mRNA-1273.351 administered through 0.5 mL intramuscular injection in the deltoid muscle on Day 1 in participants who received two vaccinations of mRNA-1273 in DMID Protocol 20-0003 (NCT04283461). N=30.

Biological: mRNA-1273Biological: mRNA-1273.351

Arm 2A

EXPERIMENTAL

100 mcg mRNA-1273 administered through 0.5 mL intramuscular injection in the deltoid muscle on Days 1 and 29, and 50 mcg mRNA-1273.351 administered through 0.5 mL intramuscular injection in the deltoid on Day 57 in COVID-19 naïve participants. N=15

Biological: mRNA-1273Biological: mRNA-1273.351

Arm 2B

EXPERIMENTAL

50 mcg mRNA-1273 administered through 0.5 mL intramuscular injection in the deltoid muscle on Days 1 and 29, and 50 mcg mRNA-1273.351 administered through 0.5 mL intramuscular injection in the deltoid on Day 57 in COVID-19 naïve participants. N=15

Biological: mRNA-1273Biological: mRNA-1273.351

Arm 2C

EXPERIMENTAL

100 mcg mRNA-1273.351 administered through 0.5 mL intramuscular injection in the deltoid muscle on Days 1 and 29 in COVID-19 naïve participants. N=20

Biological: mRNA-1273.351

Arm 2D

EXPERIMENTAL

50 mcg mRNA-1273.351 administered through 0.5 mL intramuscular injection in the deltoid muscle on Days 1 and 29 in COVID-19 naïve participants. N=20

Biological: mRNA-1273.351

Arm 2E

EXPERIMENTAL

100 mcg mRNA-1273 administered through 0.5 mL intramuscular injection in the deltoid muscle on Day 1, and 100 mcg mRNA-1273.351 administered through 0.5 mL intramuscular injection in the deltoid on Day 29 in COVID-19 naïve participants. N=20

Biological: mRNA-1273Biological: mRNA-1273.351

Arm 2F

EXPERIMENTAL

50 mcg mRNA-1273 administered through 0.5 mL intramuscular injection in the deltoid muscle on Day 1, and 50 mcg mRNA-1273.351 administered through 0.5 mL intramuscular injection in the deltoid on Day 29 in COVID-19 naïve participants. N=20

Biological: mRNA-1273Biological: mRNA-1273.351

Arm 2G

EXPERIMENTAL

50 mcg of mRNA-1273 and 50 mcg of mRNA-1273.351 administered through 0.5 mL intramuscular injection in the deltoid muscle on Days 1 and 29 in COVID-19 naïve participants. N=20

Biological: mRNA-1273Biological: mRNA-1273.351

Arm 2H

EXPERIMENTAL

25 mcg of mRNA-1273 and 25 mcg of mRNA-1273.351 administered through 0.5 mL intramuscular injection in the deltoid muscle on Days 1 and 29 in COVID-19 naïve participants. N=20

Biological: mRNA-1273Biological: mRNA-1273.351

Interventions

mRNA-1273BIOLOGICAL

Lipid nanoparticle (LNP) dispersion containing an mRNA that encodes for the pre fusion stabilized spike protein of the Wuhan-Hu-1 strain of SARS-CoV-2. mRNA-1273 consists of an mRNA Drug Substance that is manufactured into LNPs composed of the proprietary ionizable lipid, SM-102, and 3 commercially available lipids, cholesterol, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), and PEG2000 DMG.

Arm 1BArm 2AArm 2BArm 2EArm 2FArm 2GArm 2H
mRNA-1273.351BIOLOGICAL

Lipid nanoparticle (LNP) dispersion containing an mRNA that encodes for the pre fusion stabilized spike protein of the B.1.351 variant SARS-CoV-2 strain. mRNA-1273.351 consists of an mRNA Drug Substance that is manufactured into LNPs composed of the proprietary ionizable lipid, SM-102, and 3 commercially available lipids, cholesterol, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), and PEG2000 DMG.

Arm 1AArm 1BArm 2AArm 2BArm 2CArm 2DArm 2EArm 2FArm 2GArm 2H

Eligibility Criteria

Age18 Years - 99 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Provides written informed consent prior to initiation of any study procedures.
  • Be able to understand and agrees to comply with planned study procedures and be available for all study visits.
  • Agrees to the collection of venous blood per protocol.
  • Cohort 1: previously received 2 doses of mRNA-1273 intramuscular (IM) as part of DMID 20-0003.
  • Cohort 1: Male or non-pregnant female, \>/= 18 years of age at time of enrollment. Cohort 2: Male or non-pregnant female, 18 through 55 years of age at time of enrollment.
  • Women of childbearing potential\* must agree to practice abstinence or use at least one acceptable primary form of contraception.\*\*, \*\*\* Note: These criteria are applicable to females in a heterosexual relationship and child-bearing potential (i.e., the criteria do not apply to subjects in a same sex relationship).
  • \* Not of childbearing potential - post-menopausal females (defined as having a history of amenorrhea for at least one year) or a documented status as being surgically sterile (hysterectomy, bilateral oophorectomy, tubal ligation/salpingectomy, or Essure(R) placement).
  • \*\* Acceptable forms of primary contraception include monogamous relationship with a vasectomized partner who has been vasectomized for 180 days or more prior to the subject's first vaccination, intrauterine devices, birth control pills, and injectable/implantable/insertable hormonal birth control products.
  • \*\*\* Must use at least one acceptable primary form of contraception for at least 30 days prior to the first vaccination and at least one acceptable primary form of contraception for 60 days after the last vaccination.
  • In good health.\*
  • Oral temperature is less than 100.0 degrees Fahrenheit (37.8 degrees Celsius).
  • Must agree to have samples stored for secondary research.
  • Agrees to adhere to Lifestyle Considerations throughout study duration.
  • Must agree to refrain from donating blood or plasma during the study (outside of this study).

You may not qualify if:

  • Positive pregnancy test prior to each vaccine administration.
  • BMI \> 40.0 kg / m\^2.
  • Female subject who is breastfeeding.
  • Has any medical disease or condition that, in the opinion of the participating site PI or appropriate sub-investigator, precludes study participation.\*
  • \* Including acute, subacute, intermittent or chronic medical disease or condition that would place the subject at an unacceptable risk of injury, render the subject unable to meet the requirements of the protocol, or may interfere with the evaluation of responses or the subject's successful completion of this trial.
  • Presence of self-reported or medically documented significant medical or psychiatric condition(s).\*
  • \* Significant medical or psychiatric conditions include but are not limited to: Respiratory disease (e.g., chronic obstructive pulmonary disease \[COPD\], asthma) requiring daily medications currently or any treatment of respiratory disease exacerbations (e.g., asthma exacerbation) in the last 5 years. Asthma medications: inhaled, oral, or intravenous (IV) corticosteroids, leukotriene modifiers, long and short acting beta agonists, theophylline, ipratropium, biologics.
  • Significant cardiovascular disease (e.g., congestive heart failure, cardiomyopathy, ischemic heart disease), history of myocarditis or pericarditis as an adult, myocardial infarction (MI) within past 6 months, coronary artery bypass surgery or stent placement, or uncontrolled cardiac arrhythmia.
  • Neurological or neurodevelopmental conditions (e.g., history of migraines in the past 5 years, epilepsy, stroke, seizures in the last 3 years, encephalopathy, focal neurologic deficits, Guillain-Barré syndrome, encephalomyelitis, transverse myelitis, stroke or transient ischemic attack, multiple sclerosis, Parkinson's disease, amyotrophic lateral sclerosis, Creutzfeldt-Jakob disease, or Alzheimer's disease).
  • Ongoing malignancy or recent diagnosis of malignancy in the last five years excluding basal cell and squamous cell carcinoma of the skin, which are allowed.
  • An autoimmune disease, including hypothyroidism without a defined non-autoimmune cause, localized or history of psoriasis.
  • An immunodeficiency of any cause. Chronic kidney disease, estimated glomerular filtration rate (eGFR) \< 60 mL / min / 1.73m\^2.
  • Type 2 diabetes mellitus, not including prediabetes.
  • Has an acute illness\*, as determined by the participating site PI or appropriate sub-investigator, with or without fever \[oral temperature \>/= 38.0 degrees Celsius (100.4 degrees Fahrenheit)\] within 72 hours prior to each vaccination.
  • \* An acute illness which is nearly resolved with only minor residual symptoms remaining is allowable if, in the opinion of the participating site PI or appropriate sub-investigator, the residual symptoms will not interfere with the ability to assess safety parameters as required by the protocol.
  • +17 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (4)

The Hope Clinic of Emory University

Decatur, Georgia, 30030-1705, United States

Location

Cincinnati Children's Hospital Medical Center Vaccine Research Center

Cincinnati, Ohio, 45229-3039, United States

Location

Vanderbilt University - Pediatric - Vanderbilt Vaccine Research Center

Nashville, Tennessee, 37232-0004, United States

Location

Kaiser Permanente Washington Health Research Institute

Seattle, Washington, 98101-1466, United States

Location

Related Publications (1)

  • Anderson E, Jackson L, Rouphael N, Widge A, Montefiori D, Doria-Rose N, Suthar M, Cohen K, O'Connell S, Makowski M, Makhene M, Buchanan W, Spearman P, Creech CB, O'Dell S, Schmidt S, Leav B, Bennett H, Pajon R, Posavad C, Hural J, Beigel J, Albert J, Abebe K, Eaton A, Rostad C, Rebolledo P, Kamidani S, Graciaa D, Coler R, McDermott A, Ledgerwood J, Mascola J, DeRosa S, Neuzil K, McElrath MJ, Roberts P. Safety and Immunogenicity of a Third Dose of SARS-CoV-2 mRNA Vaccine - An Interim Analysis. Res Sq [Preprint]. 2022 May 3:rs.3.rs-1222037. doi: 10.21203/rs.3.rs-1222037/v1.

MeSH Terms

Conditions

COVID-19Coronavirus Infections

Interventions

2019-nCoV Vaccine mRNA-1273

Condition Hierarchy (Ancestors)

Pneumonia, ViralPneumoniaRespiratory Tract InfectionsInfectionsVirus DiseasesCoronaviridae InfectionsNidovirales InfectionsRNA Virus InfectionsLung DiseasesRespiratory Tract Diseases

Intervention Hierarchy (Ancestors)

mRNA VaccinesNucleic Acid-Based VaccinesVaccines, SyntheticRecombinant ProteinsProteinsAmino Acids, Peptides, and ProteinsVaccinesBiological ProductsComplex MixturesCOVID-19 VaccinesViral VaccinesAntigensBiological Factors

Results Point of Contact

Title
Lisa Jackson
Organization
Kaiser Permanente Washington

Publication Agreements

PI is Sponsor Employee
No
Restriction Type
LTE60
Restrictive Agreement
Yes

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
NONE
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
NIH
Responsible Party
SPONSOR

Study Record Dates

First Submitted

March 4, 2021

First Posted

March 5, 2021

Study Start

March 31, 2021

Primary Completion

July 5, 2022

Study Completion

July 5, 2022

Last Updated

March 18, 2024

Results First Posted

September 13, 2023

Record last verified: 2021-04-06

Locations