NCT04675281

Brief Summary

The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a new coronavirus discovered in December 2019 in Wuhan, China and currently responsible of a worldwide outbreak and the death of more than 55,000 patients in France. The more severe form of COVID-19 disease induces a pneumonia with profound hypoxemia which may require invasive mechanical ventilation. It is estimated that 5% of COVID-19 patients are admitted to the Intensive Care Unit (ICU) for management. Hospital mortality in patients who develop severe acute respiratory distress syndrome (ARDS) ranges between 40% and 60%. The investigators purpose to investigate the pathological findings of COVID-19 patients who died from ARDS in the ICU by doing post-mortem lung biopsies

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
170

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Apr 2020

Shorter than P25 for all trials

Geographic Reach
1 country

44 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

April 22, 2020

Completed
8 months until next milestone

First Submitted

Initial submission to the registry

December 15, 2020

Completed
4 days until next milestone

First Posted

Study publicly available on registry

December 19, 2020

Completed
2 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 3, 2021

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

March 3, 2021

Completed
Last Updated

November 30, 2023

Status Verified

November 1, 2023

Enrollment Period

11 months

First QC Date

December 15, 2020

Last Update Submit

November 29, 2023

Conditions

Keywords

SARS-CoV-2PneumoniaIntensive care unitsAcute Respiratory Distress SyndromeHistopathology

Outcome Measures

Primary Outcomes (1)

  • Analysis of the pathological findings of a large cohort of patients who died from COVID-19 in the ICU

    Detailed description and analysis of the primary lesions of the lungs in patients who died in the ICU from Covid-19.

    Two post-mortem lung biopsies performed immediately after death.

Secondary Outcomes (8)

  • Comparison between early (<1 week after ICU admission) and late (≥1 week) deaths

    Two post-mortem lung biopsies performed immediately after death.

  • Analysis of the influence of ARDS severity and length on pathological findings

    Two post-mortem lung biopsies performed immediately after death.

  • Analysis of the influence of ARDS severity and length on pathological findings

    Two post-mortem lung biopsies performed immediately after death.

  • Analysis of the influence of pharmacological treatments (steroids) on pathological findings

    Two post-mortem lung biopsies performed immediately after death.

  • Analysis of the correlation between radiological findings and pathological findings

    Two post-mortem lung biopsies performed immediately after death.

  • +3 more secondary outcomes

Study Arms (1)

Critically-ill adult patients who died in the Intensive Care Unit from a documented COVID-19

Other: 2 post-mortem transcutaneous lung biopsies (1 anterior ; 1 posterior) using anatomical landmarks

Interventions

All specimens are fixed with 4% neutral formaldehyde and embedded in paraffin wax in the department of pathology of each participating centre. Afterwards, all samples are sent for analysis to the department of pathology of Nantes university hospital. All biopsies are independently analysed by a group of pathologists who are experts in lung tissue and blinded to clinical information. All biopsies will be analysed using a predetermined semi-quantitative histological scoring grid. The pathologists are asked to assess the degree of: edema, cell necrosis, hyaline membrane formation, proliferation of alveolar type 2 cells, fibrosis, capillary congestion, alveolar edema, neutrophilic infiltration, and microscopic thrombosis. Finally, the following patterns are recorded: exudative diffuse alveolar damage (DAD), proliferative DAD, fibrosis, intra-alveolar haemorrhage, lymphocytic pneumonia, organizing pneumonia, acute fibrinous organizing pneumonia (AFOP), thrombotic microangiopathy.

Critically-ill adult patients who died in the Intensive Care Unit from a documented COVID-19

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Patients deceased in the ICU from a Covid-19 during the study period

You may qualify if:

  • Adult patients (Age≥18 years-old)
  • Hospitalized in Intensive Care Unit (ICU)
  • Dead in the ICU from documented Covid-19 (clinical and radiological signs of pneumonia with a positive SARS-Cov-2 PCR from the upper or lower respiratory tract)
  • Not registered in the national register of refusal of the French Biomedicine Agency
  • According to French law, there was no requirement for signed consent, but the patient's next of kin were informed about the study before enrolment and were given a letter of information about the study.

You may not qualify if:

  • Covid-19 not documented by a positive SARS-Cov-2 PCR
  • Patient with a positive SARS-Cov-2 PCR but without any signs of pneumonia during the ICU stay (no clinical and no radiological signs of pneumonia)
  • Patient registered in the "national register of refusal" of the French Biomedicine Agency
  • Refusal expressed by the patient's next of kin to participate to the study

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (44)

CH Amiens

Amiens, France

Location

Angers University Hospital

Angers, France

Location

CH Angoulême

Angoulême, France

Location

CH Annecy

Annecy, France

Location

Hopital Privé d'Antony

Antony, France

Location

CH Argenteuil

Argenteuil, France

Location

CH Belfort

Belfort, France

Location

CHU Bordeaux

Bordeaux, France

Location

Hopital Sainte Camille

Bry-sur-Marne, France

Location

CH Cahors

Cahors, France

Location

CH Cergy Pontoise

Cergy-Pontoise, France

Location

CH Cholet

Cholet, France

Location

CHU Clermont-Ferrand

Clermont-Ferrand, France

Location

CH Compiègne-Noyon

Compiègne, France

Location

CHD Vendée

La Roche-sur-Yon, France

Location

Hopital Lyon Sud

Lyon, France

Location

Hôpital Lyon Sud

Lyon, France

Location

Marseille Hopital Nord APHM

Marseillette, France

Location

CH Melun

Melun, France

Location

CH Montélimar

Montélimar, France

Location

Nantes University Hospital

Nantes, 44000, France

Location

CHU Nice

Nice, France

Location

CHR Orléans

Orléans, France

Location

CH Ambroise Paré APHP

Paris, France

Location

GHEF Marne La Vallée

Paris, France

Location

Hopital Antoine Béclère APHP

Paris, France

Location

Hopital Cochin APHP

Paris, France

Location

Hopital Georges Pompidou APHP

Paris, France

Location

Hopital La Pitié Salpetrière APHP

Paris, France

Location

Hopital Louis Mourier APHP

Paris, France

Location

Hopital Necker APHP

Paris, France

Location

Hopital Saint-Antoine APHP

Paris, France

Location

Hopital Saint-Louis APHP

Paris, France

Location

CH Poissy

Poissy, France

Location

Hopital Privé Claude Galien

Quincy-sous-Sénart, France

Location

CH Reims

Reims, France

Location

CHU Rennes

Rennes, France

Location

CH Saint-Brieuc

Saint-Brieuc, France

Location

CHU Saint-Etienne

Saint-Etienne, France

Location

Hopital Foch

Suresnes, France

Location

CHRU Tours

Tours, France

Location

CH Troyes

Troyes, France

Location

CH Vannes

Vannes, France

Location

CH Versailles

Versailles, France

Location

Related Publications (1)

  • Morin J, Sagan C, Pere M, Chelha R, Olivier PY, Simon G, Rougon M, Pene F, Ferre A, Kamel T, Boyer A, Badie J, Hayon J, Decavele M, Garcon P, Richard JC, Argaud L, Hraiech S, Auchabie J, Foucault C, Legay F, Chanareille PM, Souweine B, Geri G, Delbove A, Bonny V, Beuret P, Vimpere D, Plantefeve G, Canet E. Post-mortem lung biopsies in fatal Covid-19 acute respiratory distress syndrome: a prospective cohort study of 169 patients (HISTOCOVID). Ann Intensive Care. 2025 Jun 11;15(1):80. doi: 10.1186/s13613-025-01493-5.

Biospecimen

Retention: SAMPLES WITH DNA

Lung biopsies

MeSH Terms

Conditions

COVID-19Respiratory Distress SyndromePneumonia

Condition Hierarchy (Ancestors)

Pneumonia, ViralRespiratory Tract InfectionsInfectionsVirus DiseasesCoronavirus InfectionsCoronaviridae InfectionsNidovirales InfectionsRNA Virus InfectionsLung DiseasesRespiratory Tract DiseasesRespiration Disorders

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

December 15, 2020

First Posted

December 19, 2020

Study Start

April 22, 2020

Primary Completion

March 3, 2021

Study Completion

March 3, 2021

Last Updated

November 30, 2023

Record last verified: 2023-11

Data Sharing

IPD Sharing
Will share

Locations