NCT04619524

Brief Summary

Analysis of proteins from cervical mucus will be done in patients undergoing infertility treatment (fresh or frozen embryo transfer). Cervical mucus will be analysed for potential new biomarkers of endometrium receptivity. Comparison of the peptide spectrum will be done for the pregnant and not pregnant patients.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
476

participants targeted

Target at P75+ for not_applicable

Timeline
8mo left

Started May 2018

Longer than P75 for not_applicable

Geographic Reach
1 country

3 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Progress93%
May 2018Dec 2026

Study Start

First participant enrolled

May 1, 2018

Completed
2.5 years until next milestone

First Submitted

Initial submission to the registry

November 2, 2020

Completed
4 days until next milestone

First Posted

Study publicly available on registry

November 6, 2020

Completed
6.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2026

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2026

Last Updated

March 20, 2026

Status Verified

March 1, 2026

Enrollment Period

8.7 years

First QC Date

November 2, 2020

Last Update Submit

March 19, 2026

Conditions

Keywords

cervixmucusendometriumreceptivitybiomarkerproteomicsinfertilityin-vitrofertilisation

Outcome Measures

Primary Outcomes (1)

  • Mass spectrometer analysis of the proteins from cervical mucus

    Highly sensitive mass analysis of the proteins from cervical mucus on Thermo Orbitrap Elite instrument for all collected samples.

    48 months

Secondary Outcomes (2)

  • Comparison of the protein analysis between the pregnant and not pregnant patients

    48 months

  • Detection of the new endometrial receptivity biomarkers.

    48 months

Study Arms (2)

A: Patients undergo cycle with the transfer of fresh embryos

EXPERIMENTAL

In study group A the cervical mucus will be collected from patients undergoing the in vitro fertilisation (IVF)/intracytoplasmic sperm injection (ICSI)/embryo transfer (ET) cycle with the transfer of fresh embryos.

Diagnostic Test: Cervical mucus will be collected from patients undergoing IVF

B: Patients undergo cycle with the transfer of frozen embryos

EXPERIMENTAL

In study group B cervical mucus will be sampled from patients undergoing treatment cycles with the transfer of cryopreserved embryos.

Diagnostic Test: Cervical mucus will be collected from patients undergoing transfer of cryopreserved embryos

Interventions

Patients undergoing hormonal substitution for transfer of cryopreserved embryos will be sampled for cervical mucus.

B: Patients undergo cycle with the transfer of frozen embryos

Patients undergoing hormonal stimulation for IVF will be sampled for cervical mucus.

A: Patients undergo cycle with the transfer of fresh embryos

Eligibility Criteria

AgeUp to 36 Years
Sexfemale(Gender-based eligibility)
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64)

You may qualify if:

  • female aged less than 37 years (maximally 36y + 364d)
  • no smoker
  • normal menstrual cycles lasting between 25 to 35 days
  • had been infertile for less than five years
  • normal responder
  • fewer than three failed cycles of assisted reproduction treatment, including fresh IVF/ intracytoplasmic sperm injection (ICSI) embryo transfer cycles and/or frozen-thawed embryo transfer cycles
  • sperm obtained through ejaculation
  • spermiogram more than 5 million sperm/mL
  • BMI 19-29 kg/m2
  • follicle stimulating hormone (FSH) \< 10 IU/L on the third day
  • basal antral follicle count of 5-15
  • undergoing the same routine gonadotrophin-releasing hormone agonist (GnRHa) long depot or gonadotrophin-releasing hormone antagonist (GnRH-ant.) protocol
  • informed consent

You may not qualify if:

  • genetic disease
  • metabolic and/or endocrine disorders
  • polycystic ovary syndrome (defined by the Rotterdam criteria)
  • women with prior diagnosis of endometriosis or adenomyosis
  • previous gynecological/pelvic surgery except for salpingectomy
  • repeated spontaneous abortions (two or more)
  • previously less than 5 oocytes and/or serum anti-Mullerian hormone value \< 1.0 mIU/ml or more than 20 oocytes, milli-International unit (mIU)
  • previous ovarian hyperstimulation syndrome (OHSS)
  • presence of any structural abnormality of the reproductive system
  • donor oocyte cycles
  • severe male factor infertility \< 5 million sperm/mL
  • low response to stimulation
  • endometrium \< 8 mm at the day of human chorionic gonadotropin (hCG) or ET
  • number of retrieved oocytes 5 - 20
  • low fertilization capacity (rate of fertilization \< 20% and late ICSI following IVF fertilization failure)
  • +30 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (3)

Brno University Hospital

Brno, South Moravian, 62500, Czechia

RECRUITING

University Hospital Olomouc

Olomouc, 77900, Czechia

RECRUITING

University Hospital Olomouc

Olomouc, Czechia

RECRUITING

MeSH Terms

Conditions

Infertility

Condition Hierarchy (Ancestors)

Genital DiseasesUrogenital Diseases

Study Officials

  • Marian Hajduch, MD.PhD.

    Palacky University in Olomouc, Faculty of Medicine and Dentristry

    STUDY DIRECTOR

Central Study Contacts

Radovan Pilka, Prof.MD.PhD.

CONTACT

Petr Dzubak, MD.PhD.

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

November 2, 2020

First Posted

November 6, 2020

Study Start

May 1, 2018

Primary Completion (Estimated)

December 31, 2026

Study Completion (Estimated)

December 31, 2026

Last Updated

March 20, 2026

Record last verified: 2026-03

Locations