Gingival Crevicular Fluid Characterization During Orthodontic Treatment

NCT04226469 | Completed Results

• 1 other identifier: 2013-0183
• Study Type: observational
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

During Orthodontic tooth movement, teeth are moved through alveolar bone under applied forces. The applied mechanical loading force must be transferred to the alveolar bone via periodontal ligament (PDL). This process of mechanotransduction stimulates bone remodeling during which osteoblasts produce bone on the tension side and osteoclasts resorb bone on the compression side of the PDL. Complex interactions between osteoblasts and osteoclasts involve numerous biologic molecules including cytokines and growth factors. During the tooth movement, the expression of cytokines such as interleukin (IL)-1β, IL-6, IL-8, prostaglandin E2, RANKL and MMP1 in PDL will be up-regulated. The sequence of events from the mechanotransduction commanding the tightly controlled accomplishment of osteogenesis attention sides and osteoclastogenesis at compressive sides is not completely understood. The gingival crevicular fluid (GCF) is a transudate of interstitial tissues that is produced by an osmotic gradient and it is released into the crevicular crevices at a flow rate of about 3 ul/h. Orthodontic treatment is triggered by an inflammatory process and it has been hypothesized that the quantification of specific biomarkers within the GCF can be determined using Periotron. However contrasting results have been reported in the literature, which studies showing both increased or unchanged GCF volumes incident to orthodontic treatment. Given that the orthodontic treatment is triggered by a set of inflammatory cytokines that are released into the crevicular fluid during the mechanical loading, and its homeostasis is dependent on mechanical stimulation. An understanding of the biological response of crevicular fluid to mechanical loading could further advance the knowledge of orthodontic treatment. In this study, the investigators will investigate the biological response of gingival crevicular fluid before and after the initial wire placement of orthodontic treatment to determine the differentially expressed genes and proteins related to mechanotransduction.

Conditions

Healthy; Maxillary First Premolar Tooth Extraction; Canine Retraction

Outcome Measures

Primary Outcomes (3)

• The Distance of Tooth Movement

  The distance of tooth movement from initial timepoint to the end of study

  7 weeks

• The Fold Change of Secretory microRNA Expression

  The fold change of secretory microRNA expression compared to the initial level

  7 weeks

• The Amount of Crevicular Fluid

  the volume of crevicular fluid during the tooth movement

  7 weeks

Study Arms (1)

Subjects require canine retraction

Subjects require canine retraction during orthodontic tooth movement and their gingival crevicular fluid is collected during the tooth movement. The control is the initial timepoint.

Procedure: Tooth movement

Interventions

Tooth movement PROCEDURE

The subject's canines will be retracted distally and crevicular fluid is collected

Subjects require canine retraction

Eligibility Criteria

• Age: 11 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64)
• Sampling Method: Probability Sample

Study Population

Healthy, non-smoking patients aged 11-35 years old who require orthodontic treatment with first premolar extraction at the orthodontic clinic at College of Dentistry, University of Illinois at Chicago (UIC)

You may qualify if:

• Healthy, non-smoking patients aged 11-35 years old who require orthodontic treatment with the first premolar extraction at the orthodontic clinic at College of Dentistry, the University of Illinois at Chicago (UIC)

You may not qualify if:

• Craniofacial syndromic subjects
• periodontal pathology
• systemic problems and underlying diseases
• smoking

Sponsors & Collaborators

• University of Illinois at Chicago: lead

Study Sites (1)

Department of Orthodontics, Clinic

Chicago, Illinois, 60612, United States

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Gingival crevicular fluid

MeSH Terms

Interventions

Tooth Movement Techniques

Intervention Hierarchy (Ancestors)

Orthodontics, Corrective; Orthodontics; Dentistry

Results Point of Contact

• Title: Phimon Atsawasuwan
• Organization: University of Illinois Chicago

patsawas@uic.edu

3124132651

Study Officials

• Phimon Atsawasuwan, DDS, MS, PhD

  University of Illinois at Chicago

  PRINCIPAL INVESTIGATOR

Publication Agreements

• PI is Sponsor Employee: No
• Restrictive Agreement: No

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: January 6, 2020
• First Posted: January 13, 2020
• Study Start: January 1, 2019
• Primary Completion: May 5, 2021
• Study Completion: May 5, 2021
• Last Updated: May 7, 2025
• Results First Posted: May 7, 2025

Record last verified: 2025-05

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)