Time-course of Mitochondrial Biogenic Gene and Protein Expression in Exercised Human Skeletal Muscle

NCT03975777 | Unknown

• 1 other identifier: QMPL-TC-19
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

This study examines the impact of exercise intensity on the 12-hour time-course of mitochondrial biogenic gene and protein expression in human skeletal muscle. Briefly, participants will perform two experimental sessions involving an acute bout of work-matched low- or high-intensity interval cycling exercise in a randomized crossover fashion, two weeks apart. Skeletal muscle biopsies will be obtained from the vastus lateralis (3 from each leg) before and 1, 3, 6, 9, and 12 hours post-exercise. Changes in the expression of various transcription factors and mitochondrial proteins will be examined at the mRNA and protein level to determine the time-course of changes in these factors differs between exercise protocols of different intensities.

Conditions

Mitochondrial Biogenesis

Outcome Measures

Primary Outcomes (1)

• Change in peroxisome proliferator activated receptor (PPAR) gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression

  PGC-1α gene expression (indirect indicator of PGC-1α activity). Transcriptional co-activator involved in the control of mitochondrial biogenesis.

  before exercise to 1, 3, 6, 9, and 12 hours post-exercise

Secondary Outcomes (21)

• Change in PGC-1α protein content

  before exercise to 1, 3, 6, 9, and 12 hours post-exercise

• Change in nuclear factor-erythroid 2 like 2 (Nrf2) mRNA expression

  before exercise to 1, 3, 6, 9, and 12 hours post-exercise

• Change in Nrf2 protein content

  before exercise to 1, 3, 6, 9, and 12 hours post-exercise

• Change in PPAR-beta mRNA expression

  before exercise to 1, 3, 6, 9, and 12 hours post-exercise

• Change in estrogen related receptor (ERR)-gamma mRNA expression

  before exercise and 1, 3, 6, 9, and 12 hours post-exercise

Study Arms (2)

low-intensity exercise - high-intensity exercise

EXPERIMENTAL

low-intensity exercise session followed by a high-intensity exercise session separated by a minimum of 14 days

Other: LO; Other: HI

high-intensity exercise - low-intensity exercise

EXPERIMENTAL

high-intensity exercise session followed by a low-intensity exercise session separated by a minimum of 14 days

Other: LO; Other: HI

Interventions

LO OTHER

Participants will perform an acute bout of low-intensity cycling (11 x 1 min intervals at \~73% of peak aerobic work rate interspersed with 1 min recovery periods)

high-intensity exercise - low-intensity exercise; low-intensity exercise - high-intensity exercise

HI OTHER

Participants will perform an acute bout of high-intensity cycling (8 x 1 min intervals at \~100% of peak aerobic work rate interspersed with 1 min recovery periods)

high-intensity exercise - low-intensity exercise; low-intensity exercise - high-intensity exercise

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• recreationally active (no more than three hours of structured weekly physical activity)
• not currently involved in a systematic training program for improving cardiorespiratory fitness, muscular strength, and/or sport-specific skills
• body mass index \< 30 kg/m2

You may not qualify if:

• presence of cardiometabolic disease
• taking regular prescription medication for the treatment of cardiometabolic disease
• current cigarette smoker

Sponsors & Collaborators

• Brendon Gurd, PhD: lead

Study Sites (1)

School of Kinesiology and Health Studies

Kingston, Ontario, K7L3N6, Canada

RECRUITING

Study Officials

• Brendon J. Gurd, PhD

  School of Kinesiology and Health Studies

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Hashim Islam, MSc

CONTACT

4037954125; 16mhi@queensu.ca

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: May 29, 2019
• First Posted: June 5, 2019
• Study Start: June 1, 2019
• Primary Completion: April 30, 2020
• Study Completion: April 30, 2020
• Last Updated: March 4, 2020

Record last verified: 2020-03

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)