Impact of Exercise Intensity on the Time-course of Mitochondrial Biogenic Gene and Protein Expression in Human Skeletal Muscle.
Sponsor study identifier
interventional
10
1 country
1
Study Start
June 1, 2019
Actual (reported)Primary Completion
April 30, 2020
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
Study Completion
April 30, 2020
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
This study examines the impact of exercise intensity on the 12-hour time-course of mitochondrial biogenic gene and protein expression in human skeletal muscle. Briefly, participants will perform two experimental sessions involving an acute bout of work-matched low- or high-intensity interval cycling exercise in a randomized crossover fashion, two weeks apart. Skeletal muscle biopsies will be obtained from the vastus lateralis (3 from each leg) before and 1, 3, 6, 9, and 12 hours post-exercise. Changes in the expression of various transcription factors and mitochondrial proteins will be examined at the mRNA and protein level to determine the time-course of changes in these factors differs between exercise protocols of different intensities.
Registry-reported information. Elapsed time and enrollment totals alone do not measure study progress or recruitment pace.
April 30, 2020
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Dates and qualifiers reported by the registry. Estimated dates do not confirm that a milestone has occurred.
May 29, 2019
Actual (reported)June 1, 2019
Actual (reported)June 5, 2019
Actual (reported)April 30, 2020
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
April 30, 2020
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
Change in peroxisome proliferator activated receptor (PPAR) gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression
PGC-1α gene expression (indirect indicator of PGC-1α activity). Transcriptional co-activator involved in the control of mitochondrial biogenesis.
before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Change in PGC-1α protein content
before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Change in nuclear factor-erythroid 2 like 2 (Nrf2) mRNA expression
before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Change in Nrf2 protein content
before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Change in PPAR-beta mRNA expression
before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Change in estrogen related receptor (ERR)-gamma mRNA expression
before exercise and 1, 3, 6, 9, and 12 hours post-exercise
low-intensity exercise session followed by a high-intensity exercise session separated by a minimum of 14 days
high-intensity exercise session followed by a low-intensity exercise session separated by a minimum of 14 days
Participants will perform an acute bout of low-intensity cycling (11 x 1 min intervals at \~73% of peak aerobic work rate interspersed with 1 min recovery periods)
Participants will perform an acute bout of high-intensity cycling (8 x 1 min intervals at \~100% of peak aerobic work rate interspersed with 1 min recovery periods)
School of Kinesiology and Health Studies
Kingston, Ontario, K7L3N6, Canada
Brendon J. Gurd, PhD
School of Kinesiology and Health Studies
First Submitted
May 29, 2019
First Posted
June 5, 2019
Study Start
June 1, 2019
Actual (reported)Primary Completion
April 30, 2020
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This estimate has passed. The registry has not confirmed an actual date for this milestone.
Study Completion
April 30, 2020
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
Last Updated
March 4, 2020
Record last verified: 2020-03
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Source: ClinicalTrials.gov.
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