Examining the Impact of Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males

NCT03717948 | Completed

• 2 other identifiers: BFRNP402635
• Study Type: interventional
• Target: 13
• Locations: 1 country
• Sites: 1

Brief Summary

This crossover study investigates the effects of restricting arterial blood flow during cycling exercise on PGC-1α and its upstream signalling pathways. In a randomized order, participants will exercise once for 30 minutes with restricted blood flow and then will exercise once for 30 minutes with no blood flow restriction.

Conditions

Mitochondrial Biogenesis

Keywords

Acute Exercise; Mitochondria; Skeletal Muscle; PGC1α; Mitochondrial Biogenesis; Intracellular Signalling

Outcome Measures

Primary Outcomes (1)

• Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA)

  Fold-change in PGC-1α mRNA expression

  15 minutes before exercise and 3 hours post-exercise for both exercise sessions

Secondary Outcomes (10)

• Catecholamine concentrations

  15 minutes before exercise and immediately post-exercise for both exercise sessions

• Total and phosphorylated p38 mitogen-activated protein kinase (MAPK)

  15 minutes before exercise and immediately post-exercise for both exercise sessions

• Total and phosphorylated acetyl coenzyme A carboxylase (ACC)

  15 minutes before exercise and immediately post-exercise for both exercise sessions

• Total and phosphorylated protein kinase A (PKA)

  15 minutes before exercise and immediately post-exercise for both exercise sessions

• Acetylated p53

  15 minutes before exercise and immediately post-exercise for both exercise sessions

Study Arms (2)

CTL - BFR

EXPERIMENTAL

CTL Exercise then BFR Exercise

Other: BFR Exercise; Other: CTL Exercise

BFR - CTL

EXPERIMENTAL

BFR Exercise then CTL Exercise

Other: BFR Exercise; Other: CTL Exercise

Interventions

BFR Exercise OTHER

Participants will exercise on a recumbent bike placed on a 45 degree incline (BFR).

BFR - CTL; CTL - BFR

CTL Exercise OTHER

Participants will exercise on a recumbent bike placed on flat ground (CTL).

BFR - CTL; CTL - BFR

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• hours of physical activity per week
• No previous cycling training
• No concurrent involvement in another training program
• Body mass index \< 30 kg/m2

You may not qualify if:

• Presence of cardiovascular disease
• Presence of metabolic disease
• Taking regular oral medication
• Current smoker

Sponsors & Collaborators

• Queen's University: lead

Study Sites (1)

Queen's University

Kingston, Ontario, K7L3N6, Canada

Location

MeSH Terms

Interventions

Blood Flow Restriction Therapy

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities

Study Officials

• Brendon J Gurd, PhD

  Queen's University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: INVESTIGATOR, OUTCOMES ASSESSOR
• Masking Details: Allocation concealment, assessor blinding
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: October 19, 2018
• First Posted: October 24, 2018
• Study Start: October 1, 2018
• Primary Completion: January 28, 2019
• Study Completion: January 28, 2019
• Last Updated: July 15, 2019

Record last verified: 2019-07

Locations

CA (1)