NCT03918200

Brief Summary

High follicular fluid amyloid A level is associated with reduced pregnancy rate. The aim of this study was to find an association between serum level of amyloid A and unexplained infertility.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
90

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Oct 2018

Shorter than P25 for all trials

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

October 1, 2018

Completed
5 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 10, 2019

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

March 10, 2019

Completed
1 month until next milestone

First Submitted

Initial submission to the registry

April 15, 2019

Completed
2 days until next milestone

First Posted

Study publicly available on registry

April 17, 2019

Completed
Last Updated

April 17, 2019

Status Verified

April 1, 2019

Enrollment Period

5 months

First QC Date

April 15, 2019

Last Update Submit

April 16, 2019

Conditions

Outcome Measures

Primary Outcomes (1)

  • Difference between SAA level between cases and control.

    1 day (once)

Secondary Outcomes (1)

  • Correlation between SAA level with other parameters in the hormonal profile (e.g., FSH, LH, E2).

    1 day (once)

Study Arms (2)

Study

Women with unexplained infertility

Other: Serum amyloid A level

Control

Fertile women who had normal physical and pelvic examination, regular menstrual cycles, don't use hormonal contraceptive, had one child at least.

Other: Serum amyloid A level

Interventions

For determination of SAA level, a 5-ml sample of venous blood will be taken from each participant under aseptic conditions, and centrifuged at 2500 g for 15 minutes at 4°C c. separated into serum aliquots,and stored frozen at -80°C until analysis of SAA ,which will be done by a double-antibody sandwich Enzyme Linked Immunosorbent Assay (ELISA) kits supplied by cusabio,USA according to manufacturers instruction .Each sample was tested in duplicate and the average will be taken. A cut off level of 30 mg/L SAA level will be chosen to reflect a real increase in its level

ControlStudy

Eligibility Criteria

AgeUp to 40 Years
Sexfemale
Healthy VolunteersYes
Age GroupsChild (0-17), Adult (18-64)
Sampling MethodProbability Sample
Study Population

Infertile women attending Ain Shams University infertility outpatient clinic seeking fertility and fulfilling the criteria.

You may qualify if:

  • women with unexplained infertility (unprotected, regular intercourse for at least 2 years).
  • Nulligravida ,Regular mensturation.
  • Age less than 40 years .
  • Normal parameters (in the study group):
  • hormonal profile (day 2 FSH,LH), midluteal phase progesterone, Prolactin, TSH
  • transvaginal ultrasound
  • hysterosalpingography
  • detailed semen analysis

You may not qualify if:

  • Age above 40
  • Smoking females
  • Obesity (BMI\>30)
  • Women with any systemic or medical diseases leads to elevation of SAA as:
  • Systemic lupus erythematosis
  • Rheumatoid arthritis
  • Sarcoidosis
  • Diabetes Mellitus
  • Using hormonal contraception (for control group)

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Ain Shams University Hospital

Cairo, Egypt

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

serum sample

MeSH Terms

Conditions

Infertility

Condition Hierarchy (Ancestors)

Genital DiseasesUrogenital Diseases

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Doctor

Study Record Dates

First Submitted

April 15, 2019

First Posted

April 17, 2019

Study Start

October 1, 2018

Primary Completion

March 10, 2019

Study Completion

March 10, 2019

Last Updated

April 17, 2019

Record last verified: 2019-04

Locations