SIT LESS 4: How Does Reducing Sitting Time Improve Glucose and Lipid Metabolism?

NCT03912922 | Completed

• 1 other identifier: NL62413.068.17
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

Research has shown that replacing sitting time with low intensity physical activity (such as slowly walking and standing) has beneficial effects on metabolic health, like insulin sensitivity, comparable to improvements after sitting all day in combination with 1h streneous exercise. The main objective of this study is to investigate the underlying mechanisms responsible for improved insulin sensitivity after 4 days of sitting less compared to sitting and exercise in healthy obese women. Our secondary objective is to investigate the effects of sitting less on cardio metabolic parameters.

Conditions

Overweight and Obesity

Keywords

insulin sensitivity; sitting time

Outcome Measures

Primary Outcomes (1)

• Insulin sensitivity

  2-step hyperinsulinemic euglycemic clamp

  Day 5 of intervention period

Secondary Outcomes (5)

• ex vivo skeletal muscle mitochondrial respiration

  Day 5 of intervention period

• substrate oxidation

  Day 5 of intervention period

• Intrahepatic lipid content

  Day 5 of intervention period

• Blood pressure

  Day 5 of intervention period

• Plasma cardio metabolic markers

  Day 5 of intervention period

Study Arms (3)

Sit regime

EXPERIMENTAL

Subjects will follow the sit regime during four days. Each day consists of 14 hours sitting, 1 hour walking and 1 hour standing and 8 hours sleeping.

Other: Activity regime

Sit less regime

EXPERIMENTAL

Subjects will follow the sit less regime during four days. Each day will consist of 9 hours sitting, 3 hours walking, 4 hours standing and 8 hours sleeping.

Other: Activity regime

Exercise regime

EXPERIMENTAL

Subjects will follow the exercise regime during four days. Each day will consist of 13 hours sitting, 1 hour walking, 1 hour standing, 1 hour exercise and 8 hours sleeping. The cycle session will be at approximately 60% maximal power. Exact cycling intensity and duration will be calculated to ensure equal total energy expenditure between the sitting less and the exercise regime.

Other: Activity regime

Interventions

Activity regime OTHER

Activity regime of 4 days followed by a test day.

Exercise regime; Sit less regime; Sit regime

Eligibility Criteria

• Age: 45 Years - 70 Years
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Signed informed consent
• Caucasian
• Postmenopausal women
• Aged 45-70 years at start of the study
• Body mass index (BMI) 25 - 35 kg/m2
• Stable dietary habits (no weight loss or gain \>5kg in the past 3 months)
• Sedentary lifestyle (not more than 2,5 hours of exercise per week in the past 3 months)

You may not qualify if:

• Not able to complete the sitless try-out day according to the protocol
• Active diseases (cardiovascular, diabetes, liver, kidney, cancer or other)
• No use of medication interfering with investigated study parameters (as determined by responsible physician)
• Alcohol consumption of \>2 servings per day
• Smoking in the past 6 months
• Participation in another biomedical trial which may have an effect on insulin sensitivity less than one month before the start of the study (screening visit)
• Mental or physical disability which interferes with physical activity
• Subjects with contra-indications for magnetic resonance imaging (MRI)
• Reported participation in night shift work 2 weeks prior to the start of the study (screening visit) and during study participation. Night work is defined as working between midnight and 6.00 AM.

Sponsors & Collaborators

• Maastricht University Medical Center: lead
• Dutch Heart Foundation: collaborator

Study Sites (1)

Maastricht University Medical Centre

Maastricht, Netherlands

Location

Related Publications (1)

• Remie CME, Janssens GE, Bilet L, van Weeghel M, Duvivier BMFM, de Wit VHW, Connell NJ, Jorgensen JA, Schomakers BV, Schrauwen-Hinderling VB, Hoeks J, Hesselink MKC, Phielix E, Houtkooper RH, Schrauwen P. Sitting less elicits metabolic responses similar to exercise and enhances insulin sensitivity in postmenopausal women. Diabetologia. 2021 Dec;64(12):2817-2828. doi: 10.1007/s00125-021-05558-5. Epub 2021 Sep 12.

  PMID: 34510226 DERIVED

MeSH Terms

Conditions

Overweight; Obesity; Insulin Resistance

Condition Hierarchy (Ancestors)

Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Hyperinsulinism; Glucose Metabolism Disorders; Metabolic Diseases

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: PREVENTION
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: April 3, 2019
• First Posted: April 11, 2019
• Study Start: December 15, 2017
• Primary Completion: March 13, 2020
• Study Completion: March 13, 2020
• Last Updated: January 20, 2021

Record last verified: 2021-01

Locations

NL (1)