Dental Plaque Microbiota in Caries-free and Caries-active Children

NCT03906981 | Completed

• 1 other identifier: DD01/14
• Study Type: observational
• Target: 120
• Locations: 0 countries
• Sites: N/A

Brief Summary

Dental caries is a polymicrobial infection originally thought to be caused by Streptococcus mutans and Lactobacilli. However, unprecedented developments in modern molecular techniques have demonstrated that several microbial species are associated with the disease in addition to streptococci and lactobacilli. Dental caries initiation and progression is a dynamic process in which demineralization of the tooth structure, as a result of acid production from acidogenic bacteria (due to carbohydrate metabolism) is being counteracted by the remineralization of the tooth surface by strong alkali production by certain bacteria in the dental biofilm. The aim of the present study is to identify the different microbiota in the oral biofilm using new laboratory techniques as well as the levels of salivary proteins in caries-free compared with caries active Arab children.

Conditions

Caries; Plaque

Outcome Measures

Primary Outcomes (1)

• Dental plaque microbiota profile

  Identification of dental plaque microbiota profile using HOMINGS

  2 months

Study Arms (2)

Caries free

6-9 year old caries free children

Other: Plaque from each subject will be collected but there will be no intervention

Caries active

6-9 year-old caries active (\>5 dmft/DMFT) children

Other: Plaque from each subject will be collected but there will be no intervention

Interventions

Plaque from each subject will be collected but there will be no intervention OTHER

No intervention

Caries active; Caries free

Eligibility Criteria

• Age: 6 Years - 9 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17)
• Sampling Method: Probability Sample

Study Population

Pupils from randomly selected public schools

You may qualify if:

• Arab 6-9 year old children
• Healthy
• Caries free, dmft/DMFT=0
• Caries Active: dmft/DMFT\>5

You may not qualify if:

• Children with a systemic disease or any drug use that could affect the microflora of the oral cavity or the salivary gland functions
• History of salivary gland diseases
• Those who received antibiotic therapy during the last 3 months
• Children with severe localized or generalized periodontitis.

Sponsors & Collaborators

• Kuwait University: lead

Biospecimen

Retention: SAMPLES WITH DNA

Plaque sample from each subject

MeSH Terms

Conditions

Plaque, Amyloid

Condition Hierarchy (Ancestors)

Pathological Conditions, Anatomical; Pathological Conditions, Signs and Symptoms

Study Officials

• Muawia A Qudeimat, MDentSci

  Kuwait University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: April 5, 2019
• First Posted: April 8, 2019
• Study Start: January 1, 2016
• Primary Completion: March 1, 2019
• Study Completion: April 1, 2019
• Last Updated: September 25, 2019

Record last verified: 2019-09