Culture System for Isolating Circulating Tumor Cells

NCT03843450 | Unknown

• 1 other identifier: 201811053RINA
• Study Type: observational
• Target: 90
• Locations: 1 country
• Sites: 1

Brief Summary

This culture system utilizes the special affinity difference of biomedical material coating for different cells to achieve the effect of isolating cancer cells from the blood sample. The coating of the system has the characteristic that to make the WBCs adhesion, but the cancer cells in the blood sample suspend in the culture medium, which achieves the effect of separating cancer cells from the blood. The supernatant with the cancer cells can further be isolated from the cultural system for related analysis and detection to achieve early diagnosis and screening.

Conditions

Early Detection of Cancer

Keywords

circulating tumor cells,WBCs adhesion, biomedical materials

Outcome Measures

Primary Outcomes (2)

• Cancer Cell Isolation Efficiency of The Isolation System

  The percentage isolation of fluorescently-labeled A549 cell lines will be spiked into the whole blood, and the put into the system. The supernatant of the system will be collected to re-culture on the culture dishes and analyzed by fluorescence microscope.

  1 day

• WBCs adhesion ratio

  The whole blood will be diluted and put into the system. And the supernatant will be collected to count the number of blood cell number by the automatic blood cell count.

  1 hour

Eligibility Criteria

• Age: 20 Years - 70 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

The study population will include polycythemia patients between the ages of 20 and 70. We just use the waste blood from patients' routine phlebotomy therapy. Also, to protect the vulnerable subjects, we don't recruit minors, prisoners, aborigines, pregnant women, people with mental disorders, students, and subordinates, etc.

You may qualify if:

• Polycythemia patients are between the ages of 20 and 70.
• Willing to sign the consent form under the informed consent.

You may not qualify if:

• \. Vulnerable Subjects included minors, prisoners, aborigines, pregnant women, people with mental disorders, students, and subordinates, etc.

Sponsors & Collaborators

• National Taiwan University Hospital: lead

Study Sites (1)

National Taiwan University Hospital

Taipei, Taiwan

RECRUITING

Study Officials

• Tai-Horng Young

  Institute of Biomedical Engineering, National Taiwan University

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Tai-Horng Young

CONTACT

23123456; thyoung@ntu.edu.tw

Yin-Tzu Chen

CONTACT

886-2-23123456; R07528032@ntu.edu.tw

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 14, 2019
• First Posted: February 18, 2019
• Study Start: January 29, 2019
• Primary Completion: July 1, 2022
• Study Completion: September 26, 2022
• Last Updated: November 5, 2020

Record last verified: 2020-09

Locations

TW (1)