Impact of Periodontal Treatment on the RANKL/OPG Ratio in Crevicular Fluid

NCT03787875 | Completed

• 1 other identifier: UV2
• Study Type: observational
• Target: 40
• Locations: 0 countries
• Sites: N/A

Brief Summary

Objectives: Alveolar resorption is one of the most important events in periodontitis. Osteoclast activity is regulated by the ratio between receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG). The aim of this study was to evaluate changes in the RANKL/OPG ratio in crevicular fluid after periodontal treatment. Material and methods: A total of 15 patients with periodontitis were included in the study group. Samples were collected from an area with active periodontitis and a healthy area. The RANKL and OPG levels were measured before and after periodontal scaling and root planing (SRP) treatment. The study group was compared to the control group, which included 10 patients without periodontitis.

Conditions

Periodontal Diseases; Biomarkers; Bone Resorption

Keywords

RANKL; OPG; periodontal biomarkers; alveolar bone loss; chronic periodontitis

Outcome Measures

Primary Outcomes (2)

• Levels of RANKL in crevicular fluid

  receptor activator of NF-κB ligand.RANKL is a protein that determines the activation of osteoclasts by binding to the RANK membrane receptor that preosteoclates possess. The ranks wi be measured in μg / μl.

  49 days

• Levels of OPG in crevicular fluid

  osteoprotegerin.Osteoprogesterian is a soluble protein that acts as a decoy blocked RANK receptor and thus initiating osteoclastogenesis. The opg will be measured in μg / μl.

  49 days

Secondary Outcomes (3)

• probing depth

  49 days

• clinical attachment level

  49 days

• bleeding upon probing

  49 days

Study Arms (2)

Control

The control group included 10 periodontally healthy subjects without signs or symptoms of periodontal disease.

Study

The study group included 15 subjects diagnosed with mild or advanced chronic periodontitis. Each periodontal patient had one non-affected single-rooted tooth (healthy site) and another single-rooted tooth with periodontitis (periodontitis site) with the following features: Healthy site: a single-rooted tooth with probing depths below or equal to 3 mm without recession and without bleeding on probing. Periodontitis site: another single-rooted tooth from the same patient with clinical attachment loss equal to or greater than 6 mm and bleeding upon probing.

Procedure: periodontal treatment

Interventions

periodontal treatment PROCEDURE

In the study group, periodontal scaling and root planning treatment of the 4 quadrants was performed for two consecutive weeks without the use of antiseptics or antibiotics, and the patients were instructed in oral hygiene, including both brushing techniques and proximal hygiene.

Study

Eligibility Criteria

• Age: 40 Years - 60 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

The study population is the classic refreshing race of the population of Spain.

You may qualify if:

• The control group included:
• periodontally healthy subjects
• Without signs or symptoms of periodontal disease.
• The study group included:
• subjects diagnosed with mild or advanced chronic periodontitis.
• One non-affected single-rooted tooth (healthy site)
• Single-rooted tooth with periodontitis (periodontitis site) with the following features: with clinical attachment loss equal to or greater than 6 mm and bleeding upon probing.

You may not qualify if:

• Subjects with aggressive periodontitis
• Systemic diseases or consumption of drugs affecting bone metabolism
• Antibiotic, anti-inflammatory or contraceptive treatment for the last three months prior to study initiation
• Antiplatelet therapy for the last 7 days prior to study onset
• Primary or secondary occlusal trauma
• Periapical or periodontal abscess in some of the teeth included in the study
• Periodontal treatment within the last 6 months
• Orthodontic treatment
• Smoking
• Pregnant or breastfeeding.

Sponsors & Collaborators

• University of Valencia: lead

Related Publications (1)

• Lopez Roldan A, Garcia Gimenez JL, Alpiste Illueca F. Impact of periodontal treatment on the RANKL/OPG ratio in crevicular fluid. PLoS One. 2020 Jan 27;15(1):e0227757. doi: 10.1371/journal.pone.0227757. eCollection 2020.

  PMID: 31986169 DERIVED

MeSH Terms

Conditions

Periodontal Diseases; Bone Resorption; Alveolar Bone Loss; Chronic Periodontitis

Interventions

Periodontal Index

Condition Hierarchy (Ancestors)

Mouth Diseases; Stomatognathic Diseases; Bone Diseases; Musculoskeletal Diseases; Periodontal Atrophy; Periodontitis; Chronic Disease; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Dental Health Surveys; Health Surveys; Surveys and Questionnaires; Data Collection; Epidemiologic Methods; Investigative Techniques; Dentistry; Periodontics; Health Care Evaluation Mechanisms; Quality of Health Care; Health Care Quality, Access, and Evaluation; Public Health; Environment and Public Health; Public Health Dentistry

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate professor

Study Record Dates

• First Submitted: December 21, 2018
• First Posted: December 26, 2018
• Study Start: February 2, 2016
• Primary Completion: June 5, 2016
• Study Completion: November 6, 2016
• Last Updated: December 26, 2018

Record last verified: 2018-12

Data Sharing

• IPD Sharing: Will not share