Lipids and Other Bioactive Compounds of Human Breast Milk

NCT03746886 | Completed

• 1 other identifier: Korvike
• Study Type: observational
• Target: 7
• Locations: 1 country
• Sites: 1

Brief Summary

The biochemical differences in the composition of breast milk and formula have not been profoundly established in all compound groups. Increased understanding of breast milk composition, the normal variation of various bioactive compounds and their levels is required to be able to develop formulas better corresponding the breast milk, and ensure the optimal development of also the formula-fed babies.

Conditions

Breast Milk Collection

Keywords

breast milk; lipids; bioactive compounds; infant formula

Outcome Measures

Primary Outcomes (3)

• Relative proportions (%) of regioisomers within selected molecular species (acyl carbon number:number of double bonds 50:1 and 52:2) in triacylglycerols.

  Isolation of lipids from the milk with solvent extraction. Isolation of triacylglycerols with solid phase extraction. Negative ionization of the triacylglycerols with ammonia. Selection of the molecular weight species in the first quadruple of the MS/MS. Fragmentation of the selected ions with argon. Separation of the fragment ions in the second quadrupole of the MS/MS. Calculation of the relative proportions of regioisomers with a computer software.

  11/2018-12/2019

• Relative proportions (%) of fatty acids in triacylglycerols of human milk.

  Isolation of lipids from the milk with solvent extraction. Isolation of triacylglycerols with solid phase extraction. Quantitation with gas chromatograph coupled with flame ionization detector in relation to external standards.

  11/2018-12/2019

• Quantity (mg/ml) of lipid soluble compounds in triacylglycerols of human milk.

  Isolation of lipids from the milk with solvent extraction. Gravimetric analysis of the lipids.

  11/2018-12/2019

Study Arms (1)

Breast-feeding women

A single breast-milk sample will be collected from 20 women who breast-feed their child (0-6 months old).

Other: Breast-milk

Interventions

Breast-milk OTHER

A single breast-milk sample will be collected from 20 women. Milk samples will be pooled for analysis.

Breast-feeding women

Eligibility Criteria

• Age: 18 Years - 45 Years
• Gender Eligibility Details: Self-representation
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

18-45 years old women who breast-feed their 0-6 months old child

You may qualify if:

• breast-fed child was born full-time and is healthy.
• age of the breast-fed child is 0-6 months

You may not qualify if:

• breast-fed child was born prematurely (gestation week \<37)

Sponsors & Collaborators

• University of Turku: lead

Study Sites (1)

Department of Biochemistry, University of Turku

Turku, 20014, Finland

Location

Biospecimen

Retention: SAMPLES WITH DNA

Human breast-milk samples

MeSH Terms

Conditions

Breast Milk Expression

Interventions

Milk

Condition Hierarchy (Ancestors)

Breast Feeding; Feeding Behavior; Behavior

Intervention Hierarchy (Ancestors)

Beverages; Diet, Food, and Nutrition; Physiological Phenomena; Dairy Products; Food; Food and Beverages

Study Officials

• Kaisa M Linderborg, PhD

  University of Turku

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: OTHER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: November 7, 2018
• First Posted: November 20, 2018
• Study Start: November 1, 2018
• Primary Completion: May 24, 2022
• Study Completion: May 24, 2022
• Last Updated: May 7, 2026

Record last verified: 2021-05

Locations

FI (1)