Search for Bovine miRNA Transference to Humans

NCT03740087 | Completed

• 1 other identifier: Bovine miRNA assay FCB
• Study Type: interventional
• Target: 29
• Locations: 1 country
• Sites: 1

Brief Summary

Background: Foods derived from plants and animals contain miRNAs, and, some reports have detected diet-derived miRNAs circulating in mammalian serum. It is still unclear if the miRNAs present in food can be absorbed by the gastrointestinal tract and brought to the tissues to perform regulatory functions. The transfer of functional exogenous miRNA has been demonstrated in bacterial and viral infections but it is less well characterized in mammals. Edible bovine tissues contain unique profiles of human-homologous miRNAs that withstand cooking. If miRNAs from other species can cross the gastrointestinal barrier, it could have implications in gene regulation and health. Objective: Determine whether miRNAs from beef cross the gastrointestinal barrier and are transferred to human plasma. Methods: The investigators obtained fasting plasma from 29 healthy subjects divided in two groups: the omnivore group (6 men, 8 women) and the vegan group (8 men, 7 women; control group). Each participant was given a standard meal with or without beef depending on their group, then the plasma was collected at 2, 4 and 6 hours after the meal. The changes in the levels of of miR-1, miR-10b, miR-22, miR-92 and miR-192 were analysed by quantitative Polymerase Chain Reaction (qPCR).

Conditions

Vegan Diet

Keywords

miRNA; Xenomirs; Diet; Vegan

Outcome Measures

Primary Outcomes (1)

• Relative amount of miR-1, miR-10b, miR-22, miR-92 and miR-192 in plasma

  Quantification of miR-1, miR-10b, miR-22, miR-92 and miR-192 in plasma after meal time

  Change from Baseline microRNA levels at 2, 4 and 6 hours after intervention

Study Arms (2)

Omnivorous diet

EXPERIMENTAL

This group was assigned a meal containing beef (test meal) that consisted of 200 g roast beef with salad (lettuce, tomato, lentils) and a cup of rice.

Other: Omnivorous diet

Vegan diet

ACTIVE COMPARATOR

This group was assigned a control meal consisted of salad (lettuce, tomato, lentils) and a cup of rice.

Other: Vegan diet

Interventions

Omnivorous diet OTHER

Determine if the transfer of bovine miRNAs is carried out after a meal containing beef.

Omnivorous diet

Vegan diet OTHER

Used as a baseline for miRNA levels in blood

Vegan diet

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• For omnivorous subjects was that they should consume beef at least three times per week.
• For vegan subjects was that they should have followed a strict vegan diet for at least one year

You may not qualify if:

• For all subjects included pregnancy, menstruation during study sample collection, use of medicine or food supplements, intestinal malabsorption and intolerance to ingredients included in the diet intervention.

Sponsors & Collaborators

• Universidad Autonoma de Nuevo Leon: lead

Study Sites (1)

Cristina Rodriguez-Padilla

Monterrey, Nuevo León, 66455, Mexico

Location

Related Publications (18)

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• Zhang L, Hou D, Chen X, Li D, Zhu L, Zhang Y, Li J, Bian Z, Liang X, Cai X, Yin Y, Wang C, Zhang T, Zhu D, Zhang D, Xu J, Chen Q, Ba Y, Liu J, Wang Q, Chen J, Wang J, Wang M, Zhang Q, Zhang J, Zen K, Zhang CY. Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA. Cell Res. 2012 Jan;22(1):107-26. doi: 10.1038/cr.2011.158. Epub 2011 Sep 20.

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• Dickinson B, Zhang Y, Petrick JS, Heck G, Ivashuta S, Marshall WS. Lack of detectable oral bioavailability of plant microRNAs after feeding in mice. Nat Biotechnol. 2013 Nov;31(11):965-7. doi: 10.1038/nbt.2737. No abstract available.

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• Witwer KW, McAlexander MA, Queen SE, Adams RJ. Real-time quantitative PCR and droplet digital PCR for plant miRNAs in mammalian blood provide little evidence for general uptake of dietary miRNAs: limited evidence for general uptake of dietary plant xenomiRs. RNA Biol. 2013 Jul;10(7):1080-6. doi: 10.4161/rna.25246. Epub 2013 Jun 3.

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Related Links

• Cottrill, KA, Stephen C, Y. Diet-Derived MicroRNAs: Separating the Dream from Reality. microRNA Diagnostics and Therapeutics; 2014. p. 46-57.

MeSH Terms

Interventions

Diet, Vegan

Intervention Hierarchy (Ancestors)

Diet, Vegetarian; Diet, Plant-Based; Diet Therapy; Nutrition Therapy; Therapeutics; Diet; Nutritional Physiological Phenomena; Diet, Food, and Nutrition; Physiological Phenomena

Study Officials

• Fermín Mar-Aguilar, Dr.

  Universidad Autonoma de Nuevo Leon

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Model Details: Baseline blood samples were obtained after 12 hours overnight fast; the postprandial miRNA state was measured after intake of a meal with beef (test meal) that consisted of 200 g roast beef with salad (lettuce, tomato, lentils) and a cup of rice. The control diet consisted of the same intervention, without the roast beef. Each intervention day the meals were prepared with fresh food. Participants ate meals under control of the study staff. Postprandial samples were collected at 2, 4 and 6 hours after the diet intervention. The subjects did not eat or drink again until the end of sample collection.

Study Record Dates

• First Submitted: November 6, 2018
• First Posted: November 14, 2018
• Study Start: April 13, 2015
• Primary Completion: November 16, 2015
• Study Completion: May 22, 2016
• Last Updated: November 15, 2018

Record last verified: 2018-11

Data Sharing

• IPD Sharing: Will not share

Locations

ME (1)