Cryogenic Preservation of Spermatozoa

NCT03734120 | Completed

• 1 other identifier: CHU-413
• Study Type: observational
• Target: 52
• Locations: 1 country
• Sites: 1

Brief Summary

The reference technique for the conservation of gametes is storage in liquid nitrogen but new vats of nitrogen vapor (storage over liquid nitrogen) or in dry phase (storage in an insulated compartment of liquid nitrogen in a tank Liquid nitrogen) also allow the storage of flakes. The purpose of this work is to evaluate the dry-phase cryopreservation technique of liquid nitrogen compared with liquid-phase storage, depending on the duration of cryopreservation.

Conditions

Fertility; Spermatic Parameters

Keywords

Cryo preservation; Spermatic nuclear quality

Outcome Measures

Primary Outcomes (2)

• Sperm DNA integrity

  the spermatic DNA integrity can be quantified by TUNEL method. The result will be expressed as a percentage of fragmented DNA

  at 3 months

• Sperm DNA integrity

  the spermatic DNA integrity can be quantified by TUNEL method. The result will be expressed as a percentage of fragmented DNA

  at 6 months

Secondary Outcomes (4)

• Sperm parameter post cryopreservation

  at 3 and 6 months

• Lab parameter post cryopreservation

  at 3 and 6 months

• Spermatic DNA Compaction

  at 3 and 6 months

• Acrosomal integrity

  at 3 and 6 months

Study Arms (1)

men

men undergoing routine semen analysis for infertility

Other: cryopreservation

Interventions

cryopreservation OTHER

Samples were frozen with a programmable freezing unit. Each semen sample was divided into two aliquots. One aliquot was plunged into liquid nitrogen and the other was stored in dry-phase nitrogen for 3 or 6 month. Thawing was performed at room temperature

men

Eligibility Criteria

• Age: 18 Years - 60 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Men undergoing routine semen analysis for infertility for reproductive medicine of university hospital in Clermont-Ferrand

You may qualify if:

• Men undergoing routine semen analysis for infertility for reproductive medicine of university hospital in Clermont-Ferrand

You may not qualify if:

• women

Sponsors & Collaborators

• University Hospital, Clermont-Ferrand: lead

Study Sites (1)

Chu Clermont-Ferrand

Clermont-Ferrand, 63003, France

Location

MeSH Terms

Interventions

Cryopreservation

Intervention Hierarchy (Ancestors)

Tissue Preservation; Histocytological Preparation Techniques; Cytological Techniques; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Histological Techniques; Preservation, Biological; Therapeutics; Investigative Techniques

Study Officials

• Florence BRUGNON

  University Hospital, Clermont-Ferrand

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: October 23, 2018
• First Posted: November 7, 2018
• Study Start: June 1, 2012
• Primary Completion: August 1, 2012
• Study Completion: August 1, 2013
• Last Updated: November 7, 2018

Record last verified: 2018-11

Locations

FR (1)