Autonomic Adaptations to Hydration and Brain Functioning

NCT03525470 | Completed

• 1 other identifier: DB-0123-Hydration
• Study Type: interventional
• Target: 12
• Locations: 0 countries
• Sites: N/A

Brief Summary

The influence of relatively small decreases in hydration status have been little studied. On two occasions subjects will be allowed to lose about 0,5% of their body weight and one occasion 300ml of water will be consumed. Functional MRI will be used to monitor brain functioning when performing a mental arithmetic task. Heart rate variability will be monitored and related to differences in brain functioning when water has and has not been drunk

Conditions

Hydration

Keywords

Hydration; Brain functioning; Heart-rate variability

Outcome Measures

Primary Outcomes (2)

• BOLD signal

  Participants will be presented with an arithmetic task, modified for use in the scanner, similar to the Paced Auditory Serial Addition Task: a 'stressful' task that measures calculation ability and is known to elicit an autonomic response 35. Pairs of two digit numbers appeared on a screen in red and participants will be required to mentally add or subtract the numbers. After 2 seconds the screens will be removed and a second screen appeared containing a correct and an incorrect answer. Participants will be required to press either a left or right button to indicate which answer was correct ). The speed of presentation is designed to be just at the level that it is possible to perform the task although it required mental effort and was 'stressful'.This allows investigation of areas of the brain associated with working memory but also those associated with emotional arousal.

  One hour in scanner (1300-1400 hours)

• Heart-rate variability

  Differences in inter-beat intervals derived from RS800 Polar heart rate monitor with a sampling rate of 1000Hz. Profile to be analysed using the Kubios software

  Differences on two testing occasions (0900 and 1300 hours) will be related to BOLD

Secondary Outcomes (3)

• Ratings of ,mood and perceived effort

  Rated baseline (0830 hours), 30 mins later, 280 minutes later

• Change in body weight

  Measured at baseline (0830 hours), after 290 minutes (before urinating) and 300 mintues (after urinating)

• Osmolality

  Baseline (0830 hours) and after 300 minutes

Study Arms (2)

Water

EXPERIMENTAL

Subjects consumed water

Biological: Water

No water

EXPERIMENTAL

Subjects did not consume water

Other: No drink

Interventions

Water BIOLOGICAL

150 mil of water was drunk on two occasions

Water

No drink OTHER

Although body weight lost no drink consumed

No water

Eligibility Criteria

• Age: 20 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Self declared as healthy and not taking any medication

Sponsors & Collaborators

• Swansea University: lead

MeSH Terms

Interventions

Water

Intervention Hierarchy (Ancestors)

Hydroxides; Alkalies; Inorganic Chemicals; Anions; Ions; Electrolytes; Oxides; Oxygen Compounds

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Model Details: Cross-over design where on one occasion subjects lost 0.5% body weight,and drank 300ml water and on another occasion nothing was consumed

Study Record Dates

• First Submitted: April 13, 2018
• First Posted: May 15, 2018
• Study Start: January 1, 2016
• Primary Completion: April 1, 2018
• Study Completion: April 1, 2018
• Last Updated: May 15, 2018

Record last verified: 2018-05