NCT03432234

Brief Summary

The global objective of this coordinated project is to test the following hypotheses: 1) the lung microbiome in chronic obstructive pulmonary disease (COPD) is different in patients suffering from frequent exacerbations (FE) compared those who do not (NE ); 2) The microbial profile of patients with FE is associated with a different local and systemic inflammatory pattern; and 3) the inflammatory and immune characteristics of COPD are modulated by the intestinal microbiome. The project is based on bronchoalveolar lavage (BAL) for sampling lung microbiome, and includes analysis of regional variability (intrapulmonary) and temporal variability. The project will also assess the correlation between BAL samples obtained from the proximal airway (oral cavity, oropharynx and sputum), and the representation of lung microbiome in them. A cohort of COPD patients (n = 50 FE; n = 50 NE) and healthy subjects (n= 30), matched by age, sex and tobacco consumption. Respiratory secretion samples will be collected in clinical stability samples, with resampling in a quarter of the participants at 6-9 months. Coordinator Project: Bacterial and fungal microbiota will be determined by 16S rRNA and ITS amplification and sequencing. Virome and functional metagenomics will be analyzed in a quarter of the participants. Subprojects: Regional variability in the lung, local and systemic inflammatory response, and the relationship between the intestinal microbiome and inflammatory and clinical characteristics of the disease will be determined. The integration of the results will be performed using network medicine methodologies. The results of this project will help to understand the pathogenesis of COPD and its exacerbations with the final aim to identify new therapeutic targets.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
120

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Mar 2016

Longer than P75 for all trials

Geographic Reach
1 country

6 active sites

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

March 7, 2016

Completed
1.8 years until next milestone

First Submitted

Initial submission to the registry

January 8, 2018

Completed
1 month until next milestone

First Posted

Study publicly available on registry

February 14, 2018

Completed
11 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2018

Completed
1.9 years until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2020

Completed
Last Updated

January 23, 2020

Status Verified

January 1, 2020

Enrollment Period

2.8 years

First QC Date

January 8, 2018

Last Update Submit

January 22, 2020

Conditions

Keywords

COPDMicrobiomeExacerbation

Outcome Measures

Primary Outcomes (1)

  • Respiratory Microbiome in COPD patients with FE and NE.

    Respiratory Microbiome composition will be determined by using metagenomic aproaches.

    7 days

Secondary Outcomes (3)

  • Bacteria related to pulmonary and systemic inflamatory factors (FE vs NE)

    7 days

  • Longitudinal variability of respiratory microbiome in COPD patients (FE vs NE).

    12 months

  • Inmunological response of lung epithelial cells COPD patients after an infectious and inflammatory stimuli

    7 days

Other Outcomes (1)

  • Spatial variability of respiratory microbiome in COPD patients (FE vs NE).

    1 day

Study Arms (3)

COPD and frequent exacerbation

Patient with COPD diagnosis and at least two exacerbations by year (FE)

Other: Biomarkers

COPD no frequent exacerbation

Patient with COPD diagnosis with no frequent exacerbation, less than 2 by year (NE).

Other: Biomarkers

Healthy (control)

Healthy volunteers patients (H)

Other: Biomarkers

Interventions

This is an observational study, there is no intervention

COPD and frequent exacerbationCOPD no frequent exacerbationHealthy (control)

Eligibility Criteria

Age40 Years - 75 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Eligible subjects were COPD patients GOLD II/III in stable situation.

You may qualify if:

  • Spirometry: FEV1/FVC \< 70%; FEV1 between 50 and 80% predicted (GOLD II/III)
  • Clinical Stability (no therapeutic change and/or use of antibiotics) during 8 weeks. - The treatment received will be registered for post-hoc studies stratified by this condition.

You may not qualify if:

  • Drug addiction, alcohol abuse,
  • Use of long-term oral or nebulised antibiotic therapy.
  • History of allergies, asthma or other chronic respiratory disease, sanitary worker, working exposure to dust or fumes.
  • Other diseases: Severe cardiovascular, neurological, psychiatric, renal, hepatic or gastrointestinal , that might interfere with the daily life activities.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (6)

Corporació Sanitaria Parc Tauli

Sabadell, Barcelona, 08208, Spain

Location

Hospital Germans Trias i Pujol

Badalona, Spain

Location

Hospital Clínic

Barcelona, Spain

Location

Hospital de Bellitge

Barcelona, Spain

Location

Hospital del Mar

Barcelona, Spain

Location

Hosptial de Sant Pau

Barcelona, Spain

Location

Biospecimen

Retention: SAMPLES WITH DNA

Oral Wash, oropharingeal swab, sputum, brochoalveolar lavage, feces, plasma, serum, white blood cells.

MeSH Terms

Conditions

Pulmonary Disease, Chronic Obstructive

Interventions

Biomarkers

Condition Hierarchy (Ancestors)

Lung Diseases, ObstructiveLung DiseasesRespiratory Tract DiseasesChronic DiseaseDisease AttributesPathologic ProcessesPathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Biological Factors

Study Officials

  • Oriol Sibila

    Hospital de Sant Pau

    PRINCIPAL INVESTIGATOR
  • Rosa Faner

    Hospital Clínic

    PRINCIPAL INVESTIGATOR
  • Eduard Monso

    Hospital Parc Tauli

    STUDY DIRECTOR

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Target Duration
12 Months
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Head of Respiratory Medicine Department

Study Record Dates

First Submitted

January 8, 2018

First Posted

February 14, 2018

Study Start

March 7, 2016

Primary Completion

December 31, 2018

Study Completion

December 1, 2020

Last Updated

January 23, 2020

Record last verified: 2020-01

Locations