NCT02826330

Brief Summary

Transversal multicentric French study on the microbiota in patients with Crohn's disease and their first degree healthy relatives The primary objective is the comparison of microbiota between patients with CD, healthy controls non genetically linked and first degree healthy relatives of patients with CD.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
240

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Oct 2013

Longer than P75 for all trials

Geographic Reach
1 country

10 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

October 3, 2013

Completed
2.7 years until next milestone

First Submitted

Initial submission to the registry

June 29, 2016

Completed
12 days until next milestone

First Posted

Study publicly available on registry

July 11, 2016

Completed
2.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 3, 2019

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

April 3, 2019

Completed
Last Updated

October 11, 2019

Status Verified

October 1, 2019

Enrollment Period

5.5 years

First QC Date

June 29, 2016

Last Update Submit

October 10, 2019

Conditions

Keywords

Crohn's DiseaseHealthy relativesDysbiosisBiomarkers

Outcome Measures

Primary Outcomes (1)

  • Percentage of Lachnospiraceae bacteria in stools within 3 groups

    After extraction DNA, microbiota will be studied via study of ribosomal DNA 16S using quantitative PCR and pyroséquençage

    1 YEAR

Secondary Outcomes (4)

  • Number of bacteria Firmicutes phylum (including Faecalibacterium prausnitzii and Clostridium Leptum) in stools within 3 groups

    1 year

  • Define different genetic and serologic backgrounds within 3 groups

    1 year

  • Quantify of bacteria with invasive properties (including AIEC) within 3 groups

    1 year

  • Study of environmental risk factors within 3 groups

    1 year

Study Arms (3)

case Crohn disease

60 cases with Crohn's Disease

Other: biomarkers

first relative healthy

2 healthy relatives per CD case (total 120)

Other: biomarkers

controls

60 controls matched on gender and age with CD cases

Other: biomarkers

Interventions

fecal microbiota analysis antibodies in serum and saliva DNA polymorphisms

Also known as: Dysbiosis
case Crohn diseasecontrolsfirst relative healthy

Eligibility Criteria

Age8 Years - 50 Years
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17), Adult (18-64)
Sampling MethodNon-Probability Sample
Study Population

Transversal multicentric French study on the microbiota in patients with Crohn's disease (n=60) and their first degree healthy relatives (n=120) and controls (n=60).

You may qualify if:

  • Patient with Crohn's disease
  • Patient \> 18 years old
  • Having at least one first degree health relative
  • OK to participate to the project
  • First degree healthy relatives
  • specific clinical questionnaire and dosing fecal calprotectin to ensure the absence of inflammatory pathology.
  • OK to participate to the project

You may not qualify if:

  • Intestinal resection.
  • Pregnant or breastfeeding woman.
  • subject under guardianship
  • subject does not speak French
  • person unable to speak
  • taking antibiotics or bowel preparation will push 6 weeks stool specimens, after cessation treatments.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (10)

Amiens University & Hospital

Amiens, 80000, France

Location

cLERMONT fERRAND University Hospital

Clermont-Ferrand, 63000, France

Location

APHP Kremlin Bicêtre

Le Kremlin-Bicêtre, France

Location

CHRU,Hôpital Jeanne de Flandres

Lille, France

Location

Hôpital Claude Huriez, CHRU

Lille, France

Location

Nancy University Hospital

Nancy, 54000, France

Location

Aphp Necker

Paris, 75000, France

Location

APHP Robert Debré

Paris, 75000, France

Location

Aphp St Antoine

Paris, 75000, France

Location

Rouen University Hospital

Rouen, 76000, France

Location

Biospecimen

Retention: SAMPLES WITH DNA

Blood, saliva, stools

MeSH Terms

Conditions

Crohn DiseaseGenetic Predisposition to DiseaseDysbiosis

Interventions

Biomarkers

Condition Hierarchy (Ancestors)

Inflammatory Bowel DiseasesGastroenteritisGastrointestinal DiseasesDigestive System DiseasesIntestinal DiseasesDisease SusceptibilityDisease AttributesPathologic ProcessesPathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Biological Factors

Study Officials

  • Corinne Gower-Rousseau, MD, PhD

    University Hospital, Lille

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
FAMILY BASED
Time Perspective
CROSS SECTIONAL
Target Duration
1 Year
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 29, 2016

First Posted

July 11, 2016

Study Start

October 3, 2013

Primary Completion

April 3, 2019

Study Completion

April 3, 2019

Last Updated

October 11, 2019

Record last verified: 2019-10

Locations