NCT03349450

Brief Summary

This is a Phase 2 non-randomized, open label, uncontrolled, efficacy and safety study. Study participants will receive two priming doses of 0.5mL of DPX-Survivac 21 days apart and up to six 0.1ml maintenance injections every two months with low dose metronomic oral cyclophosphamide (50 mg BID) for one year or until disease progression, whichever occurs first. Pembrolizumab 200 mg will be administered every 3 weeks for up to one year or until disease progression, whichever occurs first.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
25

participants targeted

Target at below P25 for phase_2

Timeline
Completed

Started Mar 2018

Longer than P75 for phase_2

Geographic Reach
1 country

6 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

November 14, 2017

Completed
7 days until next milestone

First Posted

Study publicly available on registry

November 21, 2017

Completed
4 months until next milestone

Study Start

First participant enrolled

March 13, 2018

Completed
3.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

October 31, 2021

Completed
1.7 years until next milestone

Study Completion

Last participant's last visit for all outcomes

July 18, 2023

Completed
2.8 years until next milestone

Results Posted

Study results publicly available

May 6, 2026

Completed
Last Updated

May 6, 2026

Status Verified

January 1, 2026

Enrollment Period

3.6 years

First QC Date

November 14, 2017

Results QC Date

February 9, 2026

Last Update Submit

April 15, 2026

Conditions

Keywords

LymphomaDLBCLLymphoma, B-CellLymphoma, Large B-Cell, DiffuseNeoplasms by Histologic TypeNeoplasmsLymphoproliferative DisordersLymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesLymphoma, Non-HodgkinCyclophosphamidePembrolizumabImmunosuppressive AgentsImmunologic FactorsPhysiological Effects of DrugsAntirheumatic AgentsAntineoplastic Agents, AlkylatingAlkylating AgentsMolecular Mechanisms of Pharmacological ActionAntineoplastic AgentsMyeloablative AgonistsCanadaOntarioDiffuse Large B Cell Lymphomarefractoryrelapsedcheckpoint inhibitorvaccineDPX-SurvivacSurvivinImmunotherapyTransformed LymphomaDouble HitLarge Cell LymphomaKeytruda

Outcome Measures

Primary Outcomes (1)

  • Objective Response Rate Using Modified Cheson Criteria to Treatment With DPX-Survivac and Low Dose Cyclophosphamide Administered Together With Pembrolizumab in Participants With Recurrent, Survivin-expressing B Cell Lymphomas

    Objective Response Rate is the combined Complete Response (CR) and Partial Response (PR) rates using Cheson Criteria (2007) for evaluation. Site Qualified Investigators use radiological reports to calculate the decrease in tumour size from baseline at protocol specified time points.

    1 Year

Secondary Outcomes (4)

  • Duration of Response Using Modified Cheson Criteria.

    2 Years

  • Time to Next Treatment

    42 months

  • Evidence of Regression Using Waterfall Analyses

    1 year

  • Toxicity Profile

    1 year

Other Outcomes (4)

  • Changes in Circulating T Cell Immune Responses to Survivin and Relationship to Peripheral B Cell Numbers

    1 year

  • Changes in T Cell and T Cell Subset Infiltration and Gene Expression Pathways in Treatment Compared to Pre-treatment Tumour Biopsies

    1 year

  • Assess Potential Biomarkers of Immune and Clinical Response From Subject Clinical, Biological and Immunologic Data From Pre-treatment and On-treatment Tumour Biopsies

    1 year

  • +1 more other outcomes

Study Arms (1)

Single Arm-Investigational

EXPERIMENTAL

DPX-Survivac Priming dose of 0.5ml. DPX-Survivac Booster dose of 0.1ml. Pembrolizumab 200mg Intravenously. Cyclophosphamide 50mg Twice daily orally.

Biological: DPX-SurvivacBiological: PembrolizumabDrug: Cyclophosphamide 50mg

Interventions

DPX-SurvivacBIOLOGICAL

DPX-Survivac Priming dose of 0.5ml on Study days 7 and 28. DPX-Survivac Booster dose of 0.1ml on Study days 84, 140, 196, 252, 308, and 364.

Single Arm-Investigational
PembrolizumabBIOLOGICAL

Pembrolizumab 200mg administered intravenously every 3 weeks, commencing on study day 7 to a total of 18 infusions

Single Arm-Investigational

Cyclophosphamide 50mg twice daily by mouth, administered 7 days on / 7 days off, stating at study day 0, until study day 384

Single Arm-Investigational

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • In order to be eligible for participation in this trial, the subject must:
  • Be willing and able to provide written informed consent/assent for the trial.
  • Male or female 18+ years of age on day of signing informed consent and of any racial or ethnic group
  • Has:
  • A. histologically proven DLBCL with recurrence after first, second or tertiary treatment regimens for DLBCL or,
  • B. evidence of transformed lymphoma with past history of indolent lymphoma with current biopsy showing DLBCL) or,
  • C. double hit or high grade lymphomas, including Burkitts lymphoma and High Grade B-Cell lymphoma unclassifiable (with features intermediate between Burkitts and diffuse large B cell lymphoma)
  • Has had:
  • A. recurrence requiring therapy at least 90 days post aggressive first line combination chemotherapy (e.g. RCHOP, Hyper-CVAD or other aggressive "curative" combination), autologous stem cell transplantation (ASCT), CART therapy, or aggressive second line combination therapy or,
  • B. partial response or measureable disease after first line therapy (who are not candidates for ASCT) or after second or third line therapy without disease progression or,
  • C. recurrence any time after non-aggressive combination or single agent therapy with or without Rituximab (i.e. CVP, CHL or, VP16) for first, second or third line disease or,
  • D. for subjects with transformed lymphoma, a treatment for indolent lymphoma within the last 2 years
  • Have at least one measurable site of disease based on Cheson Criteria using standard CT imaging.
  • Be willing to provide tissue from a newly obtained (up to 3 months + 7 days prior to Study Day 0) biopsy of a tumour lesion. If this is not available, the patient must be willing to undergo a core biopsy prior to starting treatment. They must also be willing to provide an on-treatment biopsy.
  • Have a performance status of 0-1 on the ECOG Performance Scale.
  • +7 more criteria

You may not qualify if:

  • The subject must be excluded from participating in the trial if the subject:
  • Is currently participating and receiving study therapy or has participated in a study of an investigational agent and received study therapy or used an investigational device within 21 days of the first dose of treatment (SD0).
  • Patients eligible for possible curative therapies such as ASCT.
  • LDH greater than 5 times the upper limit of normal.
  • Has a diagnosis of immunodeficiency or is receiving systemic steroid therapy or any other form of immunosuppressive therapy within 35 days prior to the first dose of trial treatment (SD0), except that used as pre-medication for chemotherapy or contrast-enhanced studies are eligible. Subjects may be on physiologic doses of replacement prednisone or equivalent doses of corticosteroid (\<10 mg daily).
  • Has had previous allogeneic stem cell transplant
  • Has known active TB (Bacillus Tuberculosis)
  • Hypersensitivity to pembrolizumab or any of its excipients.
  • Has had a prior anti-cancer monoclonal antibody (mAb) within 21 days prior to SD0 or who has not recovered (i.e., ≤ Grade 1) from adverse events due to agents administered more than 21 days earlier.
  • Has had prior chemotherapy, targeted small molecule therapy, or radiation therapy within 21 days prior to SD0. Subjects must have recovered from all acute toxicities from prior treatments; peripheral neuropathy must be ≤ grade 2.
  • Has a known additional malignancy that is progressing or requires active treatment. Exceptions include in situ cervical cancer, basal cell carcinoma of the skin or squamous cell carcinoma of the skin that has undergone potentially curative therapy.
  • Has known active central nervous system (CNS) metastases and/or carcinomatous meningitis. Subjects with previously treated brain metastases may participate provided; they are stable (without evidence of progression by imaging) for at least four weeks prior to the first dose of trial treatment; and any neurologic symptoms have returned to baseline; have no evidence of new or enlarging brain metastases; and are not using steroids for at least 35 days prior to trial treatment.
  • Progressive CNS lymphoma requiring treatment within 35 days prior to SD0.
  • Has history of active autoimmune disease that has required systemic treatment in the past 2 years (i.e. with use of disease modifying agents, corticosteroids or immunosuppressive drugs). Replacement therapy (e.g., thyroxine, insulin, or physiologic corticosteroid replacement therapy for adrenal or pituitary insufficiency, etc.) is not considered a form of systemic treatment.
  • Has known history of, or any evidence of active, non-infectious pneumonitis.
  • +12 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (6)

Tom Baker Cancer Centre - Alberta Health Services

Calgary, Alberta, Canada

Location

Nova Scotia Health Authority: Queen Elizabeth II Health Sciences Centre

Halifax, Nova Scotia, B3H 2Y9, Canada

Location

London Health Sciences Centre

London, Ontario, N6A 5W9, Canada

Location

Ottawa Hospital Research Institute

Ottawa, Ontario, Canada

Location

Sunnybrook Health Sciences Centre, Odette Cancer Centre

Toronto, Ontario, M4N 3M5, Canada

Location

McGill University Health Centre

Montreal, Quebec, Canada

Location

Related Publications (2)

  • Amitai I, Roos K, Rashedi I, Jiang Y, Mangoff K, Klein G, Forward N, Stewart D, Laneuville P, Bence-Bruckler I, Mangel J, Tomlinson G, Berinstein NL. PD-L1 expression predicts efficacy in the phase II SPiReL trial with MVP-S, pembrolizumab, and low-dose CPA in R/R DLBCL. Eur J Haematol. 2023 Aug;111(2):191-200. doi: 10.1111/ejh.13982. Epub 2023 May 8.

  • Pandey A, Roos K, Jiang Y, Mangoff K, Klein G, Forward N, Stewart D, Laneuville P, Bence-Bruckler I, Mangel J, Tomlinson G, Berinstein NL. Characteristics of relapsed/refractory diffuse large B-cell lymphoma patients with durable responses to maveropepimut-S, pembrolizumab, and cyclophosphamide: Long-term follow-up from the SPiReL trial. EJHaem. 2024 Dec 12;6(1):e1062. doi: 10.1002/jha2.1062. eCollection 2025 Feb.

MeSH Terms

Conditions

Lymphoma, Large B-Cell, DiffuseRecurrenceLymphomaLymphoma, B-CellNeoplasms by Histologic TypeNeoplasmsLymphoproliferative DisordersLymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesLymphoma, Non-HodgkinDendritic Cell Sarcoma, Interdigitating

Interventions

pembrolizumabCyclophosphamide

Condition Hierarchy (Ancestors)

Hemic and Lymphatic DiseasesDisease AttributesPathologic ProcessesPathological Conditions, Signs and SymptomsHistiocytic Disorders, MalignantHistiocytosis

Intervention Hierarchy (Ancestors)

Phosphoramide MustardsNitrogen Mustard CompoundsMustard CompoundsHydrocarbons, HalogenatedHydrocarbonsOrganic ChemicalsPhosphoramidesOrganophosphorus Compounds

Results Point of Contact

Title
Dr. Neil Berinstein
Organization
Sunnybrook Health Sciences Centre

Study Officials

  • Neil L Berinstein, MD

    Sunnybrook Research Institute

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
phase 2
Allocation
NA
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

November 14, 2017

First Posted

November 21, 2017

Study Start

March 13, 2018

Primary Completion

October 31, 2021

Study Completion

July 18, 2023

Last Updated

May 6, 2026

Results First Posted

May 6, 2026

Record last verified: 2026-01

Data Sharing

IPD Sharing
Will share

For original data, contact SPiReL@sunnybrook.ca. Individual patient data will not be de-identified, however, data can be shared for a period of up to 5 years after trial closure with formal Confidentiality and Data Transfer agreements in place as appropriate. A data dictionary will not be shared, but the study protocol can be found on ClinicalTrials.gov with trial #NCT03349450 and the Statistical Analysis Plan can be made available with publication.

Shared Documents
STUDY PROTOCOL
Time Frame
Up to 5 years after the closure of the trial

Locations