Characteristics of Lower Respiratory Tract Escherichia Coli Isolates in Mechanically Ventilated Intensive Care Patients

NCT03303937 | Completed

• 1 other identifier: HLM_JDR8
• Study Type: observational
• Target: 289
• Locations: 0 countries
• Sites: N/A

Brief Summary

Prospective, multicenter observational study to collect Escherichia coli (E. coli) isolates originating from mechanically ventilated intensive care unit (ICU) patients; in order to characterize phenotype and genotype of E. coli strains retrieved from the lower respiratory tract of ventilated patients.

Conditions

Nosocomial Pneumonia; Ventilator Associated Pneumonia; Escherichia Coli Infections; Escherichia Coli Pneumonia; Escherichia Coli; Diarrhea, Enteroaggregative; Mechanical Ventilation Complication

Keywords

virulence factor genes;; phenotyping; genotyping; antimicrobial susceptibility

Outcome Measures

Primary Outcomes (1)

• phylogenetic group determination

  Quadruplex polymerase chain reaction (PCR) method was used to determine the E. coli phylogenetic group (A, B1, B2, C, D, E, F), or Escherichia clade I belonging

  50 minutes

Secondary Outcomes (4)

• O-type determination

  50 minutes

• virulence factor (VF) gene content determination

  90 minutes

• antimicrobial susceptibility determination

  24 hours

• presence of betalactamase

  90 minutes

Other Outcomes (6)

• phylogenetic group belonging in other existing Escherichia coli collections

  24 hours

• O-type distribution in other existing Escherichia coli collections

  24 hours

• virulence factor (VF) gene content in other existing Escherichia coli collections

  24 hours

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

any adult critically ill patient admitted to the ICU under invasive mechanical ventilation with a positive lower respiratory tract sample for Escherichia coli

You may qualify if:

• adult, admitted to the intensive care unit
• under invasive mechanical ventilation
• presence of Escherichia coli in lower respiratory tract specimen

Sponsors & Collaborators

• Hôpital Louis Mourier: lead

Related Publications (5)

• Messika J, Magdoud F, Clermont O, Margetis D, Gaudry S, Roux D, Branger C, Dreyfuss D, Denamur E, Ricard JD. Pathophysiology of Escherichia coli ventilator-associated pneumonia: implication of highly virulent extraintestinal pathogenic strains. Intensive Care Med. 2012 Dec;38(12):2007-16. doi: 10.1007/s00134-012-2699-5. Epub 2012 Sep 28.

  PMID: 23052953 BACKGROUND

• Dufour N, Clermont O, La Combe B, Messika J, Dion S, Khanna V, Denamur E, Ricard JD, Debarbieux L; ColoColi group. Bacteriophage LM33_P1, a fast-acting weapon against the pandemic ST131-O25b:H4 Escherichia coli clonal complex. J Antimicrob Chemother. 2016 Nov;71(11):3072-3080. doi: 10.1093/jac/dkw253. Epub 2016 Jul 7.

  PMID: 27387322 BACKGROUND

• Massot M, Daubie AS, Clermont O, Jaureguy F, Couffignal C, Dahbi G, Mora A, Blanco J, Branger C, Mentre F, Eddi A, Picard B, Denamur E, The Coliville Group. Phylogenetic, virulence and antibiotic resistance characteristics of commensal strain populations of Escherichia coli from community subjects in the Paris area in 2010 and evolution over 30 years. Microbiology (Reading). 2016 Apr;162(4):642-650. doi: 10.1099/mic.0.000242. Epub 2016 Jan 28.

  PMID: 26822436 BACKGROUND

• Lefort A, Panhard X, Clermont O, Woerther PL, Branger C, Mentre F, Fantin B, Wolff M, Denamur E; COLIBAFI Group. Host factors and portal of entry outweigh bacterial determinants to predict the severity of Escherichia coli bacteremia. J Clin Microbiol. 2011 Mar;49(3):777-83. doi: 10.1128/JCM.01902-10. Epub 2010 Dec 22.

  PMID: 21177892 BACKGROUND

• Royer G, Poirel L, La Combe B, Clermont O, Chau F, Mercier-Darty M, Denamur E, Nordmann P, Ricard JD, Decousser JW. Lack of association between colistin resistance and chlorhexidine reduced susceptibility in clinical isolates of Escherichia coli. J Antimicrob Chemother. 2021 Sep 15;76(10):2736-2737. doi: 10.1093/jac/dkab235. No abstract available.

  PMID: 34245272 DERIVED

MeSH Terms

Conditions

Healthcare-Associated Pneumonia; Pneumonia, Ventilator-Associated; Escherichia coli Infections; Diarrhea

Condition Hierarchy (Ancestors)

Cross Infection; Infections; Pneumonia; Respiratory Tract Infections; Lung Diseases; Respiratory Tract Diseases; Iatrogenic Disease; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Enterobacteriaceae Infections; Gram-Negative Bacterial Infections; Bacterial Infections; Bacterial Infections and Mycoses; Signs and Symptoms, Digestive; Signs and Symptoms

Study Officials

• Jean-Damien Ricard, MD, PhD

  Assistance Publique - Hôpitaux de Paris

  STUDY DIRECTOR

• Béatrice La Combe, MD

  Assistance Publique - Hôpitaux de Paris

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor of Intensive Care

Study Record Dates

• First Submitted: September 25, 2017
• First Posted: October 6, 2017
• Study Start: March 27, 2012
• Primary Completion: June 10, 2015
• Study Completion: June 6, 2016
• Last Updated: October 6, 2017

Record last verified: 2017-10